Effectiveness of Human Mesenchymal Stem Cell Therapy for Left Ventricular Dysfunction in Ischemic Cardiomyopathy is Influenced by Cell Injection Site

Castellanos, Angela; Balkan, Wayne; DiFede, DiFede L.; Hare, Joshua M.

doi:10.1016/j.cardfail.2016.06.202

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of mesenchymal stem cells (MSCs) has shown promise in the field of regenerative medicine. Studies have investigated the use of MSCs in cardiovascular events ( Castellanos et al, 2016 ), immunological dysfunctions ( Kaplan, Youd & Lodie, 2011 ; Zhao, Ren & Han, 2016 ), bone repair ( Emmet et al, 2016 ), cartilaginous and intervertebral discs ( Blanquer, Grijpma & Poot, 2015 ), tendinosis ( Peach et al, 2017 ), and hematological malignancies ( Wang, Qu & Zhao, 2012 ), among others ( Schnitzler et al, 2016 ; Squillaro, Peluso & Galderisi, 2016 ). Tissue engineering is a promising multidisciplinary field that involves the development of materials or devices capable of specific interactions within biological tissues ( Langer & Vacanti, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Silva

Leite

Carvalho

et al. 2018

PeerJ

View full text Add to dashboard Cite

BackgroundTissue engineering has been shown to exhibit great potential for the creation of biomaterials capable of developing into functional tissues. Cellular expansion and integration depends on the quality and surface-determinant factors of the scaffold, which are required for successful biological implants. The objective of this research was to characterize and evaluate the in vitro characteristics of rabbit bone marrow mesenchymal stem cells (BM-MSCs) associated with a bacterial cellulose membrane (BCM). We assessed the adhesion, expansion, and integration of the biomaterial as well as its ability to induce macrophage activation. Finally, we evaluated the cytotoxicity and toxicity of the BCM.MethodsSamples of rabbit bone marrow were collected. Mesenchymal stem cells were isolated from medullary aspirates to establish fibroblast colony-forming unit assay. Osteogenic, chondrogenic, and adipogenic differentiation was performed. Integration with the BCM was assessed by scanning electron microscopy at 1, 7, and 14 days. Cytotoxicity was assessed via the production of nitric oxide, and BCM toxicity was assessed with the MTT assay; phagocytic activity was also determined.ResultsThe fibroblastoid colony-forming unit (CFU-F) assay showed cells with a fibroblastoid morphology organized into colonies, and distributed across the culture area surface. In the growth curve, two distinct phases, lag and log phase, were observed at 15 days. Multipotentiality of the cells was evident after induction of osteogenic, chondrogenic, and adipogenic lineages. Regarding the BM-MSCs’ bioelectrical integration with the BCM, BM-MSCs were anchored in the BCM in the first 24 h. On day 7 of culture, the cytoplasm was scattered, and on day 14, the cells were fully integrated with the biomaterial. We also observed significant macrophage activation; analysis of the MTT assay and the concentration of nitric oxide revealed no cytotoxicity of the biomaterial.ConclusionThe BCM allowed the expansion and biointegration of bone marrow progenitor cells with a stable cytotoxic profile, thus presenting itself as a biomaterial with potential for tissue engineering.

show abstract

Section: Introductionmentioning

confidence: 99%

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Silva

Leite

Carvalho

et al. 2018

PeerJ

View full text Add to dashboard Cite

show abstract

“…The use of mesenchymal stem cells (MSCs) has shown promise in the field of regenerative medicine. Studies have investigated the use of MSCs in cardiovascular events (Castellanos et al, 2016), immunological dysfunctions (Kaplan, Youd & Lodie, 2011;Zao, Ren & Han, 2016), bone Manuscript to be reviewed repair (Emmet et al, 2016), cartilaginous and intervertebral discs (Blanquer, Grijpma & Poot, 2015), tendinosis (Peach et al, 2017), and hematological malignancies (Wang, Qu & Zhao, 2012), among others (Schnitzler et al, 2016;Squillaro, Peluso & Galderisi, 2016). Tissue engineering is a promising multidisciplinary field that involves the development of materials or devices capable of specific interactions within biological tissues (Langer & Vacanti, 2016).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane (v0.1)"

2018

View full text Add to dashboard Cite

Background. Tissue engineering has been shown to exhibit great potential for the creation of biomaterials capable of developing into functional tissues. Cellular expansion and integration depends on the quality and surface-determinant factors of the scaffold, which are required for successful biological implants. The objective of this research was to characterize and evaluate the in vitro characteristics of rabbit bone marrow mesenchymal stem cells (BM-MSCs) associated with a bacterial cellulose membrane (BCM). We assessed the adhesion, expansion, and integration of the biomaterial as well as its ability to induce macrophage activation. Finally, we evaluated the cytotoxicity and toxicity of the BCM. Methods. Samples of rabbit bone marrow were collected. Mesenchymal stem cells were isolated from medullary aspirates to establish fibroblast colony-forming unit assay. Osteogenic, chondrogenic, and adipogenic differentiation was performed. Integration with the BCM was assessed by scanning electron microscopy at 1, 7, and 14 days. Cytotoxicity was assessed via the production of nitric oxide, and BCM toxicity was assessed with the MTT assay; phagocytic activity was also determined. Results. The fibroblastoid colonyforming unit (CFU-F) assay showed cells with a fibroblastoid morphology organized into colonies, and distributed across the culture area surface. In the growth curve, two distinct phases, lag and log phase, were observed at 15 days. Multipotentiality of the cells was evident after induction of osteogenic, chondrogenic, and adipogenic lineages. Regarding the BM-MSCs' bioelectrical integration with the BCM, BM-MSCs were anchored in the BCM in the first 24 h. On day 7 of culture, the cytoplasm was scattered, and on day 14, the cells were fully integrated with the biomaterial. We also observed significant macrophage activation; analysis of the MTT assay and the concentration of nitric oxide revealed no cytotoxicity of the biomaterial. Conclusion. The BCM allowed the expansion and

show abstract

“…The use of mesenchymal stem cells (MSCs) has shown promise in the field of regenerative medicine. Studies have investigated the use of MSCs in cardiovascular events (Castellanos et al, 2016), immunological dysfunctions (Kaplan, Youd & Lodie, 2011;Zao, Ren & Han, 2016), bone repair (Emmet et al, 2016), cartilaginous and intervertebral discs (Blanquer, Grijpma & Poot, 2015), tendinosis (Peach et al, 2017), and hematological malignancies (Wang, Qu & Zhao, 2012), among others (Schnitzler et al, 2016;Squillaro, Peluso & Galderisi, 2016). Tissue engineering is a promising multidisciplinary field that involves the development of materials or devices capable of specific interactions within biological tissues (Langer & Vacanti, 2016).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Supplemental Information 12: Colorimetric Nitrite Dosage Produced by Macrophages Treated With Lipopolysaccharide (LPS) in the P

View full text Add to dashboard Cite

show abstract

Effectiveness of Human Mesenchymal Stem Cell Therapy for Left Ventricular Dysfunction in Ischemic Cardiomyopathy is Influenced by Cell Injection Site

Cited by 3 publications

References 0 publications

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Peer Review #1 of "Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane (v0.1)"

Untitled

Contact Info

Product

Resources

About