Effectiveness of a polyhexanide irrigation solution on methicillin‐resistant Staphylococcus aureus biofilms in a porcine wound model

Abstract

Irrigation and removal of necrotic debris can be beneficial for proper healing. It is becoming increasingly evident that wounds colonized with biofilm forming bacteria, such as Staphylococcus aureus (SA), can be more difficult to eradicate. Here we report our findings of the effects of an irrigation solution containing propyl-betaine and polyhexanide (PHMB) on methicillin-resistant Staphylococcus aureus (MRSA) biofilms in a porcine wound model. Thirty-nine deep partial thickness wounds were created with six wounds assigned to one of six treatment groups: (i) PHMB, (ii) Ringer's solution, (iii) hypochlorous acid/sodium hypochlorite, (iv) sterile water, (v) octenidine dihydrochloride, and (vi) octenilin. Wounds were inoculated with MRSA and covered with a polyurethane dressing for 24 hours to allow biofilm formation. The dressings were then removed and the wounds were irrigated twice daily for 3 days with the appropriate solution. MRSA from four wounds were recovered from each treatment group at 3 days and 6 days hours after initial treatment. Irrigation of wounds with the PHMB solution resulted in 97·85% and 99·64% reductions of MRSA at the respective 3 days and 6 days assessment times when compared to the untreated group. Both of these reductions were statistically significant compared to all other treatment groups (P values <0·05).

Preclinical evaluation of a novel silver gelling fiber dressing on Pseudomonas aeruginosa in a porcine wound infection model

“…P. aeruginosa is a gram negative biofilm‐forming bacteria commonly found in acute and chronic wounds . In this study, we evaluated the ability of the GFD Ag + to reduce P. aeruginosa using a porcine wound biofilm model . Porcine models are more relevant as pig skin is similar to human skin compared to rodent models, particularly in relation to epidermal‐dermal thickness ratios, dermal collagen, and elastic contents, similar patterns of hair follicles, blood vessels, and physical and molecular responses to various growth factors .…”

“…4,[18][19][20][21][22] In this study, we evaluated the ability of the GFD Ag + to reduce P. aeruginosa using a porcine wound biofilm model. 16,23,24 Porcine models are more relevant as pig skin is similar to human skin compared to rodent models, particularly in relation to epidermal-dermal thickness ratios, dermal collagen, and elastic contents, similar patterns of hair follicles, blood vessels, and physical and molecular responses to various growth factors. 4,15,[25][26][27][28] Importantly porcine skin heals primarily by epithelialization similarly to human skin.…”

Improved treatment strategies can result in better outcomes following one-stage exchange surgery for MRSA periprosthetic joint infection

“…We used systemic rifampicin in the majority (93.1 %) of cases for an average of 23.7 days [28]. Prior to the reimplantation of new components, the surgical field was also irrigated with a computed formulation of polyhexanide [29, 30]. The type of local antibiotic used was mostly similar for the patients ().…”

Evaluación in vitro de la actividad antibacteriana de diversas suturas quirúrgicas provistas de clorhexidina frente a triclosán

“…demostraron que la solución Prontosan tiene baja toxicidad celular y actúa eficazmente frente a diversos microorganismos grampositivos y gramnegativos [27] . La efectividad de este antiséptico también se ha demostrado bajo condiciones in vivo, tras observarse que la irrigación con Prontosan reducía significativamente la carga bacteriana, en un modelo porcino de infección cutánea causada por S. aureus resistente a meticilina [28] .…”