Effective technological pectinases by Aspergillus carneus NRC1 utilizing the Egyptian orange juice industry scraps

El‐Sheekh, Mostafa M.; Ismail, Abdel‐Mohsen S.; El-Abd, Mostafa A; Hegazy, El‐Sayed A.; El‐Diwany, Ahmed I.

doi:10.1016/j.ibiod.2008.06.002

Cited by 31 publications

(21 citation statements)

References 24 publications

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“…Apart from that, the complex nature of the OP eliminated the addition of mineral salts or high amount of nitrogen sources to the culture medium [2]. El-Sheekh et al [5] also used OP powder efficiently as the sole carbon source for pectinase production by A. carneus, while no activity was detected when the OP was replaced by mono-and disaccharides. Same authors also demonstrated that pectinase activity was stimulated, whereas cellulase and hemicellulase activities were inhibited by AS [5].…”

Section: Resultsmentioning

confidence: 99%

“…El-Sheekh et al [5] also used OP powder efficiently as the sole carbon source for pectinase production by A. carneus, while no activity was detected when the OP was replaced by mono-and disaccharides. Same authors also demonstrated that pectinase activity was stimulated, whereas cellulase and hemicellulase activities were inhibited by AS [5]. In another study, the same amount of (15 g/L) OP and pectin yielded similar PG activity values; however, activity was less when they were supplemented by glucose, peptone and yeast extract [33].…”

Section: Resultsmentioning

confidence: 99%

“…The presence of high amount of soluble and insoluble carbohydrates in the citrus peel makes it an attractive substrate for bioprocesses. It can be utilized directly for the production of hydrolytic enzymes [3][4][5][6][7][8]. The insoluble polysaccharides of OP such as cellulose, hemicelluloses and pectin can be degraded to sugars by pretreatments and enzymatic hydrolysis, and used for the production of value-added products such as ethanol and various enzymes [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

Büyükkileci

Lahore

Tarı

2014

Bioprocess Biosyst Eng

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The production of exo-polygalacturonase (exo-PG) from orange peel (OP), a food industrial waste, using Aspergillus sojae was studied in submerged culture. A simple, low-cost, industrially significant medium formulation, composed of only OP and (NH 4 ) 2 SO 4 (AS) was developed. At an inoculum size of 2.8 9 10 3 spores/mL, growth was in the form of pellets, which provided better mixing of the culture broth and higher exo-PG activity. These pellets were successfully used as an inoculum for bioreactors and 173.0 U/mL exo-PG was produced. Fed-batch cultivation further enhanced the exo-PG activity to 244.0 U/mL in 127.5 h. The final morphology in the form of pellets is significant to industrial fermentation easing the subsequent downstream processing. Furthermore, the low pH trend obtained during this fermentation serves an advantage to fungal fermentations prone to contamination problems. As a result, an economical exo-PG production process was defined utilizing a food industrial byproduct and producing high amount of enzyme.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

Büyükkileci

Lahore

Tarı

2014

Bioprocess Biosyst Eng

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“…This technique offers the possibility of processing agro-industrial residues that can be used, for instance, as substrates for enzymes production, helping in this way to minimize the pollution by removing this waste and converting it to useful products. In Egypt more than a hundred orange juice factories are widespread and produce at least 40,000 tons of orange pulps and peels annually, as highly wet wastes (El-Sheekh et al, 2009). Utilization of orange-canning wastes was reported by some authors (Pagán et al 2006;El-Sheekh et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Utilization of orange bagasse and molokhia stalk for production of pectinase enzyme

Ahmed

Mostafa

2013

Braz. J. Chem. Eng.

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-Studies were conducted on the production and extraction of exo-polygalacturonase (PG) in solidstate fermentation (SSF) using orange bagasse (Ob) and molokhia stalks (Ms) as a new solid support by Penicillium pinophlilum Hedg 3503 NRRL. The parameters affecting PG production under SSF were optimized. The maximum PG activity of ~ 3270 U/ g dry solid substrate was obtained from P. pinophlilum Hedg 3503 NRRL grown for 7 days on Ob and Ms in the ratio 1:3 (w/w), moistened with distilled water at 68.2% initial moisture content. Highest enzyme titers occurred in SSF without added nutrients, indicating nutrient sufficiency of the Ob and MS mixture to sustain growth and a high level of pectic substances which induced PG production. The extraction of PG from the fermented biomass was optimized. Among the various solvents tested, the maximum level of enzyme activity was achieved when acetate buffer (0.05 M; pH 5.0) was used. The optimum volume of buffer was 50 mL, an extraction time of 60 min was sufficient to extract most enzyme, which yielded 3269.6 U/ g dry solid substrate of enzyme activity. Repeated washes under the optimum conditions showed that most of the enzyme (about 98%) was recovered in three repeated extractions. Moreover, 68.9% of total activity was achieved in the first wash.

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“…The production of PG have been studied before from various agricultural residues such as wheat and soy bran [5,6], lemon peel [7], sugar beet [8], corn [9], orange peel and pulps [6,10]. The two common fermentation techniques to produce commercial pectinase preparations are solid-state fermentation (SSF) and submerged fermentation (SmF) [11].…”

Section: Introductionmentioning

confidence: 99%

Aspergillus sojae Tarafından Üretilen Poligalakturonazın Kısmi Saflaştırılması için Kromatografik Bir Yaklaşım

Şen¹,

Mata-Gómez²,

Rito‐Palomares³

et al. 2017

Akademik Gıda

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A Chromatographic Approach for Partial Purification of Polygalacturonase Produced byAspergillus sojae ABSTRACTThe aim of this study was to produce polygalacturonase from A. sojae mutant and partially purify the crude extract by chromatographic methods. As a preliminary step for the confirmation of its peptides, matrix-assisted laser-desorptionionization-time-of-flight mass spectrometry (Maldi-TOF MS) analysis was performed on in-gel digested sodiumdodecyl-sulphate-polyacrylamide-gel-electrophoresis (SDS-PAGE) gels. Three different carbon sources were employed in submerged and solid-state fermentations for the production of polygalacturonase. Crude extract was first purified by ion-exchange chromatography (IEXC) and followed further by size exclusion chromatography. Crude extracts obtained from sub-merged [of bitter orange peel, sugar beet molasses and (NH4)2SO4] and solid-state [of wheat bran, sugar beet and HCl] fermentation exhibited high levels of polygalacturonase enzyme activity (95.22 and 50.27 U/mL, respectively). Size exclusion of IEXC pooled fraction (180, 200 and 220 mM salt fractions) revealed the highest yield (36%) and purification fold (2.00). The likely polygalacturonase bands from SDS-PAGE were in-gel digested and analyzed by Maldi-TOF MS in route for peptides confirmation.

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Effective technological pectinases by Aspergillus carneus NRC1 utilizing the Egyptian orange juice industry scraps

Cited by 31 publications

References 24 publications

Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

Utilization of orange bagasse and molokhia stalk for production of pectinase enzyme

Aspergillus sojae Tarafından Üretilen Poligalakturonazın Kısmi Saflaştırılması için Kromatografik Bir Yaklaşım

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