Effective targeting of breast cancer cells (MCF7) via novel biogenic synthesis of gold nanoparticles using cancer-derived metabolites

Abstract

Biogenic synthesis of nanoparticles provides many advantages over synthetic nanoparticles including clean and non-toxic approaches. Nanoparticle-based application for the development of diagnostics and therapeutics is a promising field that requires further enrichment and investigation. The use of biological systems for the generation of gold nanoparticles (AuNPs) has been extensively studied. The search for a biocompatibility approach for the development of nanoparticles is of great interest since it can provide more targeting and less toxicity. Here, we reported a bio-reductive approach of gold to AuNPs using metabolites extracted from mammalian cells, which provided a simple and efficient way for the synthesis of nanomaterials. AuNPs were more efficiently synthesized by the metabolites extracted from breast cancer (MCF7) and normal fibroblasts (F180) cells when compared to metabolites extracted from cell-free supernatants. The metabolites involved in biogenic synthesis are mainly alcohols and acids. Spectroscopic characterization using UV-visible spectra, morphological characterization using electron microscopy and structural characterization using X-ray diffraction (XRD) confirmed the AuNPs synthesis from mammalian cells metabolites. AuNPs generated from MCF7 cells metabolites showed significant anticancer activities against MCF7 and low toxicity when compared to those generated from F180 cells metabolites. The results reflected the cytotoxic activities of the parent metabolites extracted from MCF7 versus those extracted from F180. Comparative metabolomics analysis indicated that MCF7-generated AuNPs harbored tetratetracontane, octacosane, and cyclotetradecane while those generated from F180 harbored a high percentage of stearic, palmitic, heptadecanoic acid. We related the variation in cytotoxic activities between cell types to the

Biosynthesis of Gold Nanoparticles by Vascular Cells in vitro

“…The mean diameters of the biosynthesized AuNPs populations are similar to AuNPs synthesized by other mammalian cell lines reported in the literature. For example, the secretome of an MCF7 cell line produced AuNPs with a mean diameter of 30.4 ± 0.6 nm ( Soliman et al, 2020 ). The size distribution of the biogenic AuNPs is broad for both BAEC and BASMC, with a range between 5 and 60 nm.…”

Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions

“…Compared to healthy control, FA metabolites profiling in oral lesions were identified following a previously described method [ 27 , 28 ]. Briefly, frozen tissues were suspended in methanol: chloroform (1:1) followed by sonication in a sonicator water bath for 1 h at room temperature.…”

“…Briefly, frozen tissues were suspended in methanol: chloroform (1:1) followed by sonication in a sonicator water bath for 1 h at room temperature. The filtered extracts were derivatized and analyzed by GC–MS following protocol established by Soliman et al, 2020 [ 27 ]. The metabolite extracts were derivatized by mixture of N -Trimethylsilyl- N -methyl trifluoroacetamide and Trimethylchlorosilane (MSTFA + 1% TMS) and analyzed by GC–MS using QP2010 gas chromatography–mass spectrometer (GC-2010 coupled with a GC–MS QP-2010 Ultra) equipped with an auto-sampler (AOC-20i + s) from Shimadzu (Tokyo, Japan), using Rtx-5 ms column (30 m length × 0.25 mm inner diameter × 0.25 µm film thickness: Restek, Bellefonte, PA).…”

Comparative Metabolomics Reveals the Microenvironment of Common T-Helper Cells and Differential Immune Cells Linked to Unique Periapical Lesions

“…GC-MS analysis was performed as previously described (34,35) using a QP2010 gas chromatography-mass spectrometer (GC-2010 coupled with a GC-MS QP-2010 Ultra) equipped with an autosampler (AOC-20i+s) from Shimadzu (Tokyo, Japan), using Rtx-5 ms column (30 m length × 0.25 mm inner diameter × 0.25 µm film thickness: Restek, Bellefonte, PA, USA). Helium (99.9% purity) was used as the carrier gas with a column flow rate of 1 ml/min.…”