Effective genetic markers for identifying the <i>Escherichia coli</i> F4ac receptor status of pigs

Hu, Dian-Ming; Rampoldi, Antonio; Bratus-Neuenschwander, Anna; Höfer, Andreas; Bertschinger, H. U.; Vögeli, P.; Neuenschwander, Stefan

doi:10.1111/age.12770

Cited by 6 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, in order to characterize the diarrhea susceptibility in the cohort based on known genetic risk loci, the FUT1 , rs335979375, polymorphism associated with resistances to ETEC F18 (Meijerink et al., 1997), and the CHCF1, rs340488770, marker associated with resistance to ETEC F4ab/ac (Hu et al., 2019) were genotyped using TaqMan‐based assays. Allele calling was performed on an Mx3000P qPCR System (Agilent, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The identification of genetic factors that influence the development of PWD can provide information that could be useful for risk assessment, treatment strategy selection, breeding, and/or disease prevention. In previous studies, resistance to E. coli F4ab/F4ac and E. coli F18 adhesion has been shown to be inherited as autosomal recessive traits (Bertschinger et al, 1993;Gibbons et al, 1977) and polymorphisms on SSC13 and SSC6 have been shown to co-segregate with E. coli F4 and F18 diarrhea, respectively (Hu et al, 2019;Meijerink et al, 1997). Despite the evidence provided with regard to the genetic etiology of ETEC, it is important to study this aspect further because of the multifactorial nature of PWD.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Towards identification of new genetic determinants for post‐weaning diarrhea in piglets

Ibragimov,

Eriksen,

Nielsen

et al. 2024

Animal Genetics

View full text Add to dashboard Cite

Post‐weaning diarrhea in pigs is a considerable challenge in the pig farming industry due to its effect on animal welfare and production costs, as well as the large volume of antibiotics, which are used to treat diarrhea in pigs after weaning. Previous studies have revealed loci on SSC6 and SSC13 associated with susceptibility to specific diarrhea causing pathogens. This study aimed to identify new genetic loci for resistance to diarrhea based on phenotypic data. In depth clinical characterization of diarrhea was performed in 257 pigs belonging to two herds during the first 14 days post weaning. The daily diarrhea assessments were used for the classification of pigs into case and control groups. Pigs were assigned to case and control groups based only on the incidence of diarrhea in the second week of the study in order to differentiate between differences in etiology. Genome‐wide association studies and metabolomics association analysis were performed in order to identify new biological determinants for diarrhea susceptibility. With the present work, we revealed a new locus for diarrhea resistance on SSC16. Furthermore, studies of metabolomics in the same pigs revealed one metabolite associated with diarrhea.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Towards identification of new genetic determinants for post‐weaning diarrhea in piglets

Ibragimov,

Eriksen,

Nielsen

et al. 2024

Animal Genetics

View full text Add to dashboard Cite

show abstract

“…The experiment was conducted in accordance with Swiss guidelines for animal welfare and was approved by the Swiss cantonal veterinary office (approval number: 2016_14_FR). To determine their susceptibility or resistance to ETEC F4ac, genotyping was performed on ear biopsies from week-old piglets [16]. Then, 72 Large White male and female piglets susceptible to ETEC F4ac were selected for the experiment.…”

Section: Methodsmentioning

confidence: 99%

Chestnut extract but not sodium salicylate decrease the severity of diarrhea and enterotoxigenic Escherichia coli F4 shedding in artificially infected piglets

Girard

Pradervand

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

19The development of alternatives to antibiotics is crucial to limiting the incidence of antimicrobial 20 resistance, especially in prophylactic and metaphylactic use to control post-weaning diarrhea 21 (PWD). Feed additives, including bioactive compounds, could be a promising alternative. This 22 study aimed to test two bioactive compounds, sodium salicylate (SA) and a chestnut extract 23 (CE) containing hydrolysable tannins, on the occurrence of PWD. At weaning, 72 piglets were 24 assigned to four treatments that combined two factors: CE supplementation (with 2% of CE 25 (CE+) or without (CE-)) and SA supplementation (with 35 mg/kg BW of SA (SA+) or without 26 (SA-)). Then, 4 days after weaning, all piglets were infected with a suspension at 10 8 CFU/ml 27 of enterotoxigenic Escherichia coli (ETEC F4ac). Each piglet had free access to an electrolyte 28 solution containing, or not, SA. This SA supplementation was administered for 5 days (i.e., 29 from the day of infection (day 0) to 4 days post-infection (day 4). During the 2 weeks post-30 infection, supplementation with SA had no effect (P > 0.05) on growth performances nor on 31 fecal scores. A significant SA × time interaction (P < 0.01) for fecal scores and the percentage 32 of diarrhea indicated that piglets with SA did not recover faster and did have a second episode 33 of diarrhea. In contrast to SA treatment, inclusion of CE increased (P < 0.05) growth 34 performances and feed intake. In the first week post-infection, CE decreased (P < 0.001) the 35 overall fecal scores, the percentage of piglets with diarrhea, the days in diarrhea, and ETEC 36 shedding in the feces. There was a SA×CE interaction (P < 0.05) for ETEC shedding, 37 suggesting a negative effect of combining SA with CE. This study highlighted that, in contrast 38 to SA, CE could represent a promising alternative to antibiotics immediately after weaning for 39 improving growth performance and reducing PWD. 41 Post-weaning diarrhea (PWD) is a major enteric disease in pig production that occurs mainly 42 during the first two weeks after weaning. This problem causes substantial economic losses 43 due to high rates of mortality and morbidity, depression of feed intake and growth, and 44 increased costs of medication [1]. 45 Apart from the multifactorial etiology owing to several stressors, including nutritional, social, 46 and environmental changes, PWD is often related to infection with specific pathogens. 47 Enterotoxigenic Escherichia coli (ETEC) is a prevalent pathogen involved in PWD [2]. For 48 instance, in Switzerland, 42.5% of weaned pigs with diarrhea were identified as having ETEC 49 [3]. Those bacteria possess fimbriae that adhere to receptors located on the apical side of the 50 enterocytes and secrete enterotoxins, including heat labile toxin (LT) and heat stable toxin 51 (STb), which results in fluid secretion into the intestinal lumen, leading to dehydration and 52 acidosis, which ultimately results in diarrhea [4, 5]. 53 Antibiotics are usually used to treat this type of infection....

show abstract

“…vet., MSD Animal Health GmbH, Lucerne, Switzerland), a suspension for injection that contains deactivated fimbria adhesins of E. coli F4ab, F4ac, F5, F6, and the LT-toxoid. To determine their susceptibility or resistance to ETEC F4ac, genotyping was performed on ear biopsies (TypiFix TM , Agrobiogen GmbH, Hilgertshausen, Germany) from week-old piglets originating from the Agroscope sow herd [16]. Briefly, piglets DNA was extracted using QIAamp DNA Mini Kit (Qiagen GmbH, Hilden, Germany).…”

Section: Animals and Housingmentioning

confidence: 99%

Chestnut extract but not sodium salicylate decreases the severity of diarrhea and enterotoxigenic Escherichia coli F4 shedding in artificially infected piglets

Girard

Pradervand

et al. 2020

PLoS ONE

Self Cite

View full text Add to dashboard Cite

The development of alternatives to antibiotics is crucial to limiting the incidence of antimicrobial resistance, especially in prophylactic and metaphylactic use to control post-weaning diarrhea (PWD). Feed additives, including bioactive compounds, could be a promising alternative. This study aimed to test two bioactive compounds, sodium salicylate (SA) and a chestnut extract (CE) containing hydrolysable tannins, on the occurrence of PWD. At weaning, 72 piglets were assigned to four treatments that combined two factors: CE supplementation (with 2% of CE (CE+) or without (CE-)) and SA supplementation (with 35 mg/kg BW of SA (SA+) or without (SA-)). Then, 4 days after weaning, all piglets were infected with a suspension at 10 8 CFU/ml of enterotoxigenic Escherichia coli (ETEC F4ac). Each piglet had free access to an electrolyte solution containing, or not, SA. This SA supplementation was administered for 5 days (i.e., from the day of infection (day 0) to 4 days post-infection (day 4). During the 2 weeks post-infection, supplementation with SA had no effect (P > 0.05) on growth performances nor on fecal scores. A significant SA × time interaction (P < 0.01) for fecal scores and the percentage of diarrhea indicated that piglets with SA did not recover faster and did have a second episode of diarrhea. In contrast to SA treatment, inclusion of CE increased (P < 0.05) growth performances and feed intake. In the first week postinfection, CE decreased (P < 0.001) the overall fecal scores, the percentage of piglets with diarrhea, the days in diarrhea, and ETEC shedding in the feces. There was a SA×CE interaction (P < 0.05) for ETEC shedding, suggesting a negative effect of combining SA with CE. This study highlighted that, in contrast to SA, CE could represent a promising alternative to antibiotics immediately after weaning for improving growth performance and reducing PWD.

show abstract

Effective genetic markers for identifying the Escherichia coli F4ac receptor status of pigs

Cited by 6 publications

References 27 publications

Towards identification of new genetic determinants for post‐weaning diarrhea in piglets

Towards identification of new genetic determinants for post‐weaning diarrhea in piglets

Chestnut extract but not sodium salicylate decrease the severity of diarrhea and enterotoxigenic Escherichia coli F4 shedding in artificially infected piglets

Chestnut extract but not sodium salicylate decreases the severity of diarrhea and enterotoxigenic Escherichia coli F4 shedding in artificially infected piglets

Contact Info

Product

Resources

About