Effect of zinc pretreatment on pulmonary endothelial cells in vitro and pulmonary function in a porcine model of endotoxemia

Krones, Carsten J.; Klosterhalfen, B.; Butz, Nick; Hoelzl, Friedrich; Junge, K.; Stumpf, M.; Peiper, Christian; Klinge, U.; Schumpelick, Volker

doi:10.1016/j.jss.2004.08.024

Cited by 31 publications

(35 citation statements)

References 25 publications

(26 reference statements)

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“…The amounts, expressed as milligrams per day of the respective mineral, were added to the record of the supplements received daily as part of the study protocol in order to quantify the total mineral intake through supplements. Thus, on average, the subjects from groups A, B, and C received a total of 3.7±11.5 mg/d, 16 A two-factor repeated-measures ANOVA was conducted to test the effects of time (month 0 vs month 6) and type of supplementation (standard vs improved vs extra Fe/Zn) on a series of variables, such as: Hb, MCV, ZPP, TS, PlZn, HZn, PlCu, hs-PCR, and leukocytes and PMN counts. The type of supplementation had no effect on any of these parameters (p>0.05; data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Recently, our group has documented a dramatic reduction of both iron [14] and zinc [15] absorption after gastric bypass. On the other hand, obesity is associated to a pro-inflammatory condition [6], but the participation of iron, zinc, and copper in inflammation is only partially known [16][17][18]. The objective of this study was to evaluate changes on trace element status and their potential association with selected inflammatory parameters in seriously obese patients after RYGBP.…”

Section: Introductionmentioning

confidence: 99%

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Trace Element Status and Inflammation Parameters after 6 Months of Roux-en-Y Gastric Bypass

et al. 2011

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trace Element Status and Inflammation Parameters after 6 Months of Roux-en-Y Gastric Bypass

et al. 2011

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“…Nonetheless, several clinical and animal studies addressed the issue of zinc supplementation after induction of infection, but without a coherent result. The observations ranged from beneficial effects (Krones et al 2005), to no impact or even deleterious outcomes (Krones et al 2004). This divergence might be due to the usage of different salt forms, partly inappropriate high doses of zinc (Krones et al 2004), and differences in zinc binding capacity.…”

Section: Discussionmentioning

confidence: 99%

Alterations in zinc binding capacity, free zinc levels and total serum zinc in a porcine model of sepsis

et al. 2015

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Zinc is crucial for immune function. In addition, the redistribution of zinc and other nutrients due to infection is an integral part of the host immune response to limit availability to pathogens. However, the major zinc binding protein albumin is down regulated during the acute phase response, implicating a decrease in zinc binding capacity. A prospective animal study with eight female German landrace pigs was conducted to investigate alterations in zinc binding capacity, total serum zinc and free zinc levels in the initial phase of sepsis. Sepsis was induced by instillation of autologous feces via midline laparotomy. Total serum zinc declined significantly after 1 h (10.89 ± 0.42 µM vs. 7.67 ± 0.41 µM, p < 0.001), total serum copper and iron reached a significant reduction at 4 h. Urinary excretion of zinc declined in line with total serum zinc. In comparison to total serum zinc, free zinc levels declined to a lesser, though significant, extent. Zinc binding capacity of serum decreased over time, whereby free zinc levels after addition of zinc correlated negatively with total serum protein and albumin levels. In addition IL-6 and TNF-α concentrations were measured and increased significantly 2 h after induction of sepsis. Hence, total serum zinc was the first marker of inflammation in our experiment, and might therefore be a promising biomarker for the early diagnosis of sepsis. Furthermore the observation of a substantially different serum free zinc homeostasis during sepsis provides valuable information for a potential therapeutic zinc supplementation, which has to take buffering capacity by serum proteins into account.

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“…Zinc supplementation did show potential to protect from LPS-induced endotoxemia. However, it seems to be of utmost importance to stay within a narrow concentration range and find an optimal time for zinc administration, otherwise zinc-treatment will worsen the inflammation it had been administered to counteract (Snyder and Walker 1976;Krones et al 2004Krones et al , 2005. Further investigation of zinc homeostasis on the level of cells as well as entire organisms will help optimizing the application of zinc.…”

Section: Discussionmentioning

confidence: 99%

Signal transduction in monocytes: the role of zinc ions

2007

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The availability of zinc has a regulatory role in the immune system. It can have either pro- or anti-inflammatory effects, which both seem to be a consequence of a direct interaction of zinc with the cytokine secretion by monocytes. In this review, the molecular basis for this effect, the interaction of zinc with the signal transduction of monocytes, is discussed. In particular, zinc seems to activate or inhibit several signaling pathways that interact with the signal transduction of pathogen sensing receptors, the so-called Toll-like receptors (TLR), which sense pathogen-derived molecular structures and, upon activation, lead to secretion of pro-inflammatory cytokines. The interaction of zinc with protein tyrosine phosphatases and protein kinase C, and a direct modulation of lipopolysaccharide binding to its receptor (TLR-4) all result in enhanced cytokine production. On the other hand, a complex interaction between zinc, NO and cyclic nucleotide signaling, and inhibition of interleukin-1 receptor associated kinase-1, and inhibitor of kappa B kinase all counteract the production of pro-inflammatory cytokines. A role for the zinc binding protein metallothionein as a regulator for intracellular zinc signaling is discussed. By acting on all these signaling molecules, the zinc status of monocytes can have a direct effect on inflammation.

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Effect of zinc pretreatment on pulmonary endothelial cells in vitro and pulmonary function in a porcine model of endotoxemia

Cited by 31 publications

References 25 publications

Trace Element Status and Inflammation Parameters after 6 Months of Roux-en-Y Gastric Bypass

Trace Element Status and Inflammation Parameters after 6 Months of Roux-en-Y Gastric Bypass

Alterations in zinc binding capacity, free zinc levels and total serum zinc in a porcine model of sepsis

Signal transduction in monocytes: the role of zinc ions

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