Effect of vitrification on meiotic maturation and expression of genes in immature goat cumulus oocyte complexes

Rao, Brahmasani Sambasiva; Mahesh, Yelisetti Uma; Charan, Katari Venu; Suman, Komjeti; Sekhar, Nithya; Shivaji, Sisinthy

doi:10.1016/j.cryobiol.2012.01.005

Cited by 52 publications

(26 citation statements)

References 35 publications

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“…Goat COC vitrified by either OPS or conventional straw showed an increase in expression of BAX, promoting degeneration and, consequently, low oocyte IVM rates. In contrast, the expression of BCL2 in oocytes vitrified with cryotop and hemistraw was superior, with a low rate of degeneration and higher IVM rate (Rao et al, 2012).…”

Section: Cryoprotectantsmentioning

confidence: 69%

“…COC vitrified using cryotop in the same cryoprotectant solution used for other techniques showed an increase in the potential to complete meiotic maturation postwarming, inferred by higher proportion of polar body extrusion and visualization of the metaphase II plate. Oocytes vitrified with OPS and conventional straw showed lower survival, maybe due to larger volume of vitrification solution (Rao et al, 2012), as reported in sheep (Ebrahimi et al, 2010a).…”

Section: Cryoprotectantsmentioning

confidence: 86%

“…The objective of these techniques is to reduce the volume of solution and improve the contact with nitrogen to increase the cooling speed and allows the occurrence of vitrification of solution even with lower concentrations of CP (less toxic). However, some aspects still need to be clarified, as demonstrated by the few studies comparing different techniques in small ruminant oocytes (Ebrahimi et al, 2012;Rao et al, 2012).…”

Section: Cryoprotectantsmentioning

confidence: 99%

“…The Table 1 compiles some studies on oocyte vitrification in goats and sheep using different CP and later in vitro analysis. (Ebrahimi et al, 2010a;Fernández-Reyez et al, 2012;Rao et al, 2012;Bhat et al, 2013). The objective of these techniques is to reduce the volume of solution and improve the contact with nitrogen to increase the cooling speed and allows the occurrence of vitrification of solution even with lower concentrations of CP (less toxic).…”

Section: Cryoprotectantsmentioning

confidence: 99%

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Factors that affect oocyte vitrification in small ruminants

Chaves

Souza-Fabjan

Mermillod

et al. 2014

RBCV

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At the end of its growth, the mammalian oocyte, include in preovulatory follicle, is the largest cell of the organism, with about 120 µm in diameter. The size of oocyte, together with the specific arrangement of its organels and cytoskeleton, makes a real challenge for cryopreservation techniques. For such large cell, methods that do not require equilibrium to cryopreservation, such as vitrification, are more promising. It is important to highlight that the cryopreservation of oocytes is more difficult than zygotes or later stage embryos and this technique is a challenging task because of oocyte sensitive nature to chilling and toxic effects of cryoprotectants. However, the development of a reliable method for oocyte cryopreservation would be an important advance in the field of reproductive biology for the preservation of genetic resources. The vitrification of mammalian oocytes is influenced by many variables, such as different cryoprotectants, vitrification techniques, presence or absence of cumulus cells, the oocyte structure, metabolism (such as level of lipid storage) and meiotic stage. Thus, all these factors should be considered to optimize the techniques and adapt them to oocytes from each species. Therefore, the present review aims to describe the main factors that affect oocyte vitrification in sheep and goats, reporting the main findings in both species, as well as perspectives of future improvements.Keywords: COC, cryopreservation, gamete, goat, sheep. ResumoNo fim do seu crescimento, o oócito mamífero é a maior célula do organismo, com cerca de 120 µm de diâmetro. O tamanho do oócito em conjunto com a disposição específica das organelas e do citoesqueleto faz com que ele represente um verdadeiro desafio para as técnicas de criopreservação. Para grandes células, métodos que não exigem equilíbrio para criopreservação, como a vitrificação, são mais promissores. É importante ressaltar que a criopreservação de oócitos é mais difícil que de zigotos ou embriões em estádios mais tardios e esta técnica representa um desafio devido à natureza sensível do oócito ao resfriamento e aos efeitos tóxicos de crioprotetores. Entretanto, o desenvolvimento de uma técnica confiável para a criopreservação oocitária representaria um avanço importante para a preservação de recursos genéticos. A vitrificação de oócitos em mamíferos é influenciada por muitas variáveis , tais como diferentes crioprotetores, técnicas de vitrificação, presença ou ausência de células do cumulus, estrutura do oócito, metabolismo (como o nível de armazenamento de lipídios) e estágio meiótico. Assim, todos esses fatores devem ser considerados quando o objetivo é otimizar as técnicas e adaptá-las para oócitos de cada espécie. Assim, a presente revisão tem como objetivo descrever os principais fatores que afetam a vitrificação de oócitos em ovinos e caprinos, relatando os principais resultados em ambas as espécies, bem como as perspectivas de melhorias futuras.

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Section: Cryoprotectantsmentioning

confidence: 69%

Section: Cryoprotectantsmentioning

confidence: 86%

Section: Cryoprotectantsmentioning

confidence: 99%

Section: Cryoprotectantsmentioning

confidence: 99%

See 2 more Smart Citations

Factors that affect oocyte vitrification in small ruminants

Chaves

Souza-Fabjan

Mermillod

et al. 2014

RBCV

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“…Goat oocytes have been vitrified using different techniques such as conventional straw vitrification (19), solid surface vitrification (20), and other methods like open pulled straw, hemistraw, cryoloop, and cryotop methods (21). Despite all these efforts, the results of vitrification in caprine species are far from being comparable to those for other domestic species.…”

Section: Introductionmentioning

confidence: 99%

Survivability of caprine oocytes vitrified in conventional and open pulled straws

Ali¹,

Razzaque²,

Sharma³

et al. 2014

Turk J Vet Anim Sci

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The aim of this study was to compare the efficacy of conventional straw vitrification with open pulled straw vitrification in terms of cryosurvivability and damages caused to the oocytes. Two hundred immature goat cumulus oocyte complexes (COCs) were vitrified in a solution of ethylene glycol, dimethyl sulfoxide, and sucrose using either conventional straws (100 COCs) or open pulled straws (100 COCs). The COCs were warmed and observed for morphological damages and viability after 7 days of preservation in liquid nitrogen. Among the 100 COCs cryopreserved in each case, only 83 COCs were recovered after warming in conventional straw vitrification as compared to 94 COCs in open pulled straw vitrification. In terms of morphological survivability, the percentage of morphologically normal oocytes was greater (P < 0.01) in the case of open pulled straw vitrification (86.2%) as compared to conventional straw vitrification (59.0%). Viability percentage of live oocytes was greater (P < 0.01) in the case of open pulled straw vitrification (90.4%) as compared to conventional straw vitrification (66.3%). The results indicate that open pulled straw vitrification is better than conventional straw vitrification for rapid freezing of immature goat COCs in terms of both morphological survival and viability.

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Vitrification of Day 2–3 Human Embryos Using Various Methods

Dalvit

2014

Vitrification in Assisted Reproduction

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Effect of vitrification on meiotic maturation and expression of genes in immature goat cumulus oocyte complexes

Cited by 52 publications

References 35 publications

Factors that affect oocyte vitrification in small ruminants

Factors that affect oocyte vitrification in small ruminants

Survivability of caprine oocytes vitrified in conventional and open pulled straws

Vitrification of Day 2–3 Human Embryos Using Various Methods

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