Effect of vitamin C in egg yolk extender on sperm motility of chilled stored bull semen

Abstract

An experiment was conducted to evaluate the effect of vitamin C in chicken and quail egg yolk extender on sperm motility in chilled stored bull semen. Semen was collected from the bull with the aid of artificial vagina and six types of extenders were prepared using chicken and quail egg yolk with each extender divided into three aliquots having 0, 3 and 6 mg/mL Vitamin C levels. Bottles containing the diluted semen using the chicken and quail egg yolk °extenders were stored in a refrigerator at 5C over a period of three days and monitored or evaluated at 0, 24, 48 and 72 hours. The result showed that there was a general decline in motility of semen as storage time increases, although samples with 6mg/mL Vitamin C levels had better results. It can be concluded that adding Vitamin C in chicken and quail egg yolk 0extender maintained motility of chilled semen even at 72 hours of preservation at 5C.

Effect of Guava Filtrate Supplementation in Tris and Citrate-Based Extenders on Spermatozoa Quality of Brangus Bull after Sex-rest

“…The vitamin C is transported into cells in an oxidized form, namely DHA through facilitative glucose transporters (Gluts), then reduced rapidly in cells and accumulated as ascorbic acid (AA) (Carcamo et al, 2004). Adding vitamin C in extender maintained motility of chilled semen at 5 o C (Achi et al, 2018), and benefic effects on motility of cryopreserved semen (Pinto et al, 2020). Therefore, it is necessary to try supplementation GF in Tris and Citrate-based extender on spermatozoa quality of Brangus bull after sex-break for more than a year.…”