Effect of Vitamin C Administration 24 Hours After 5-Fluorouracil Versus its Concomitant Administration on Cellular Proliferation, Cell Death and Lipid Peroxidation in WI-38 Human Fibroblast Cell Line

Obeid, Raneem F.; Elsharkawy, Radwa T.; El-kammar, Hala A.

doi:10.21608/ejh.2020.40744.1345

Cited by 1 publication

(2 citation statements)

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“…The cells of choice for this study were fibroblasts as they represent the most abundant cells of connective tissue in not only in the oral cavity but all over the body. In times of insult fibroblasts initially act in repairing the problem however in cases of long term irritation or pathological incidences fibroblasts the long-term dysregulated production of extracellular matrix eventually causes disfigurement or dysfunction (7) . They are as well proliferating cells and hence provide a reasonable model to study the effect of the evaluated denture base materials on cell cycle and cell death.…”

Section: Discussionmentioning

confidence: 99%

“…It has been revealed that on MMA exposure the cellular levels of glutathione (GSH), the natural free radical scavenger, is reduced while concomitantly ROS levels are elevated (5) . Consequently, liberated ROS react with cellular components, such as proteins, lipids, and even DNA, resulting in cell cycle arrest and eventually by apoptosis (6,7) .…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Denture Base Materials on Normal Human Fibroblast Cell Line: An In Vitro Comparative Study

Abdelhameed

kammar

2022

Egyptian Dental Journal

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PMMA is the most commonly used denture base material, however some concerns regarding its biocompatibility have been heavily studied over the years. ACETAL and PEEK are more recently introduced denture base material alternatives which are believed to be more biocompatible than PMMA. Aim of the study:To evaluate and compare the short-term effects of PMMA, ACETAL and PEEK on human fibroblast cell line regarding cytotoxicity via direct contact. The assessment of cell cycle progression, cell death and ROS release were used as indicators.Materials and methods: WI-38 cell line was divided equally into four groups. Group I was the control group, for groups II, III and IV the WI-38 cells were cultured with PMMA, ACETAL and PEEK, respectively. The groups were all assessed by flowcytometry for cell cycle and cell death evaluation and ELISA for determining ROS after 4 days of culturing.Results: Pairwise analysis Bonferroni Method showed that highest mean values of total cell death, ROS levels and cell cycle arrest in the G2/M phase of the cell cycle was noted in group II (PMMA/WI38). Pearson's correlation test (r test) showed that ROS levels correlated positively with total cell death and G2/M arrest. On the other hand, it correlated negatively with all the other parameters (G0/G1 and S). Conclusion:From the results of this study, we conclude that PEEK showed significantly superior biocompatibility compared to ACETAL and PMMA, enough to justify its use as a denture base material in spite of its high price.

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Section: Discussionmentioning

confidence: 99%