Effect of vegetable oils applied over acquired enamel pellicle on initial erosion

Ionta, Franciny Querobim; Alencar, Catarina Ribeiro Barros de; Val, Poliana Pacifico; Boteon, Ana Paula; Jordão, Maísa Camillo; Honório, Heitor Marques; Buzalaf, Marília Afonso Rabelo; Ríos, Daniela

doi:10.1590/1678-7757-2016-0436

Cited by 20 publications

(29 citation statements)

References 25 publications

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“…Reinforcement of the pellicle's protective properties by modifying its composition has been supposed in recent years as a promising novel approach in preventative dentistry (Siqueira et al, 2012;Vukosavljevic et al, 2014;Weber et al, 2015). Beside the application of polyphenolic agents for improving the pellicle's physiological function (Weber et al, 2015), it has been demonstrated that lipophilic components reveal the potential to modulate the process of bioadhesion on the tooth surface, and to modify the interfacial interactions between the pellicle-covered tooth and the surrounding oral environment (Wiegand et al, 2007;Hannig et al, 2012Hannig et al, , 2013Reich et al, 2013;Cukkemane et al, 2015;Valentijn-Benz et al, 2015;Ionta et al, 2017). Enrichment of lipids in the pellicle could be effective in protecting the tooth surface against demineralisation and bacterial biofilm formation .…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it has been demonstrated that sphingosine, phytosphingosine or sphinganine protect hydroxyapatite discs against acid-induced demineralisation under in vitro conditions by forming a diffusion barrier on the hydroxyapatite surface (Valentijn-Benz et al, 2015). Using an in situ/ex vivo approach it could be shown recently that also palm oil has the potential to prevent enamel erosion (Ionta et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Oil droplet formation on pellicle covered tooth surfaces studied with environmental scanning electron microscopy

Peckys

Jonge

Hannig

2019

Journal of Microscopy

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Summary Lipophilic components are known to modulate the process of bioadhesion on the tooth surface. However, the presence of lipid droplets at the acquired pellicle under oral conditions has not been demonstrated, yet. The purpose of the present study was to establish a method for direct visualisation of lipids on the surface of hydrated, pellicle covered tooth samples by environmental scanning electron microscopy (ESEM), and to use this technique for studying the effects of rinsing with edible oils on the acquired pellicle under in vivo conditions. In situ pellicle formation was performed by 3 min exposure of enamel and dentin specimens in the oral cavity of volunteers. Subsequently, the volunteers rinsed in vivo with safflower oil or linseed oil for 30 s, and the specimens were further carried intraorally for periods from 0 min up to several hours. After intraoral exposure the specimens were treated by osmium tetroxide vapour, and were subsequently analysed by ESEM. This technique was capable to directly visualise the presence of lipid droplets at the pellicle's surface under hydrated conditions. ESEM analyses revealed that surface bound nano‐ and micro‐sized lipid droplets were present at the acquired pellicle's surface even several hours after rinsing with edible oils indicating that these droplets had tightly adhered to the pellicle surface. Pellicle modification by edible oil rinsing as demonstrated in the present study might have the potential to be beneficial as an adjunct in dental prophylaxis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oil droplet formation on pellicle covered tooth surfaces studied with environmental scanning electron microscopy

Peckys

Jonge

Hannig

2019

Journal of Microscopy

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“… 16 ( Table 1 ) The lipids collaborate with other components to contribute to the permeability of the acquired salivary pellicle. 17 , 18 Permeability is the basis of the pellicle's resistance to the acid in the oral cavity. 17 , 18 Thus, acquired salivary pellicle retards the diffusion of lactic acid to the enamel pellicle.…”

Section: Composition and Structure Of Mature Salivary Pelliclementioning

confidence: 99%

“… 17 , 18 Permeability is the basis of the pellicle's resistance to the acid in the oral cavity. 17 , 18 Thus, acquired salivary pellicle retards the diffusion of lactic acid to the enamel pellicle. 17 , 18 Furthermore, lipids influence the ultrastructure of a pellicle and modify the outer layer of the pellicle via lipid micelles.…”

Section: Composition and Structure Of Mature Salivary Pelliclementioning

confidence: 99%

Acquired salivary pellicle and oral diseases: A literature review

Chawhuaveang

Yin

et al. 2021

Journal of Dental Sciences

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As soon as saliva contacts the teeth surface, salivary proteins adhere to the tooth surface to form acquired salivary pellicle. The formation of this acquired salivary pellicle is a dynamic and selective process of macromolecular adsorption and desorption. Although acquired salivary pellicle contains proteins and peptides, it also contains lipids, and other macro-molecules, all of which contribute to its protective properties. Acquired salivary pellicle is related to the development of common oral diseases, such as erosion, dental caries, and periodontal disease. Acquired salivary pellicle acts as a natural barrier to prevent a tooth's surface from making direct contact with acids and to protect it from erosive demineralization. It contributes to the control of dental erosion by modulating calcium and phosphate concentrations on the tooth surface. It also influences the initial colonizer of oral biofilm and affects the transportation pathway of the acidic products of cariogenic bacteria, which affects the development of dental caries. In addition, it influences periodontal disease by acting on the colonization of periodontal pathogens. This paper's aim is to provide an overview of the acquired salivary pellicle, highlighting its composition, structure, function, role in common oral diseases, and modification for the prevention of oral diseases.

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“…To improve this protection, different approaches have been proposed to modify the AEP. Natural oils can increase the fatty acid content of the AEP, improving its protective properties [Reich et al, 2013;Ionta et al, 2017]. Polyphenols can bind to AEP proteins, attract more proteins and thereby increase the thickness and reduce the permeability of the AEP [Joiner et al, 2003[Joiner et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

Pellicle Modification with Casein and Mucin Does Not Affect Surface Loss from Erosion and Abrasion

Baumann¹,

Schmidt²,

Carvalho³

2020

Caries Res

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Aim: A combination of the proteins casein and mucin is known to modify the salivary pellicle and improve its protection of the underlying enamel from erosion. It is so far not known if this protection is confined solely to erosion, or if it also extends to abrasion, and this in vitro study aimed at investigating this question. Methods: A total of 72 human enamel specimens were prepared and randomly assigned to four groups: pellicle (P), casein/mucin (CM), pellicle + casein/mucin (PCM), and control (Ctrl). Each specimen underwent five cycles, each cycle consisting of a pellicle/treatment part, an erosion part (3 min in 1% citric acid, pH 3.6, 25°C, 70 rpm), and an abrasion part (50 toothbrush strokes within 25 s in toothpaste slurry with a 200-g load). The pellicle/treatment part consisted of 2 h of incubation in whole human saliva for group P, 2 h of incubation (25°C, 70 rpm) in a protein mixture of 1% casein and 0.27% mucin for group CM, and 2 h of incubation in saliva followed by 2 h of incubation in the protein mixture for group PCM. The fourth group (Ctrl) served as the control and was kept in a humid chamber without saliva or protein treatment. The enamel surfaces were scanned with an optical profilometer initially and after the final cycle, and surface loss was analyzed. Furthermore, the surface microhardness (SMH) was measured initially, after each pellicle/treatment part and each erosion cycle, and after the final abrasion cycle. The results were analyzed with Kruskal-Wallis and Wilcoxon tests with Bonferroni corrections. Results: The different treatments did not show differences in surface loss and therefore did not protect enamel from surface loss by abrasion. Nonetheless, we observed differences in the SMH values, namely the Ctrl group being significantly softer than the experimental groups. Conclusion: The observed differences in SMH suggest that a different abrasion protocol could lead to differences in surface loss, and further investigation of whether and under which conditions pellicle modification leads to increased resistance to abrasion remains worthwhile.

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Effect of vegetable oils applied over acquired enamel pellicle on initial erosion

Cited by 20 publications

References 25 publications

Oil droplet formation on pellicle covered tooth surfaces studied with environmental scanning electron microscopy

Oil droplet formation on pellicle covered tooth surfaces studied with environmental scanning electron microscopy

Acquired salivary pellicle and oral diseases: A literature review

Pellicle Modification with Casein and Mucin Does Not Affect Surface Loss from Erosion and Abrasion

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