Effect of Ultrasound on the Production of Il-8, Basic FGF and Vegf

Reher, Peter; Doan, Ninh; Bradnock, B.; Meghji, S; Harris, Malcolm

doi:10.1006/cyto.1998.0444

Cited by 219 publications

(151 citation statements)

References 0 publications

Supporting

Mentioning

129

Contrasting

Unclassified

Order By: Relevance

“…VEGF also plays an important role in normal and abnormal neovascularization [11,12]. Prior studies have shown that LIPUS stimulates the production of VEGF in vitro and in vivo [21,27]. However, in our study there was no major difference in VEGF levels between the two groups.…”

Section: Discussioncontrasting

confidence: 80%

“…VEGF protein expression also was not different (p = 0.0124, Fig. 6) between the LIPUS group (23 ± 6; 95% CI, [19][20][21][22][23][24][25][26][27] and the control group (22 ± 6; 95% CI, 18-26).…”

Section: Bmp-2 and Vegf Expressionmentioning

confidence: 90%

“…The protein expression of BMP-2 in the control group (15 ± 2; 95% CI, 14-16) was lower (p \ 0.001) than in the LIPUS group (30 ± 3; 95% CI, 28-31). However, VEGF mRNA expression was not different (p = 0.114; Table 2) between the LIPUS group (28 ± 2; 95% CI, [26][27][28][29] and the control group (26 ± 3; 95% CI, [24][25][26][27][28]. VEGF protein expression also was not different (p = 0.0124, Fig.…”

Section: Bmp-2 and Vegf Expressionmentioning

confidence: 90%

“…The benefits of LIPUS have been documented during all stages of bone repair, including osteogenesis, angiogenesis, and chondrogenesis [28,29]. In vitro studies have shown that LIPUS increases bone formation, osteoblast activity, and osteogenic differentiation of mesenchymal stem cells (MSCs) [27,31]. In vivo studies have found that LIPUS treatment promotes new bone formation and improves tissue blood flow [3,16,24,34].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Low-intensity Pulsed Ultrasound Enhances Bone Repair in a Rabbit Model of Steroid-associated Osteonecrosis

Zhu

Cai

Lin

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

Background Steroids are a leading cause of femoral head osteonecrosis. Currently there are no medications available to prevent and/or treat steroid-associated osteonecrosis. Low-intensity pulsed ultrasound (LIPUS) was approved by the FDA for treating delayed union of bone fractures. Some studies have reported that LIPUS can enhance bone formation and local blood flow in an animal model of fracture healing. However, whether the effect of osteogenesis and neovascularization by LIPUS can enhance the repair progress in steroid-associated osteonecrosis is unknown.Questions/purposes We hypothesized that LIPUS may facilitate osteogenesis and neovascularization in the reparative processes of steroid-associated osteonecrosis. Using a rabbit animal model, we asked whether LIPUS affects (1) bone strength and trabecular architecture; (2) blood vessel number and diameter; and (3) BMP-2 and VEGF expression. Methods Bilateral femoral head necrosis was induced by lipopolysaccharide and methylprednisolone in 24 rabbits. The left femoral heads of rabbits received LIPUS therapy (200 mW/cm 2 ) for 20 minutes daily and were classified as the LIPUS group. The right femoral heads of the same rabbits did not receive therapy and were classified as the control group. All rabbits were euthanized 12 weeks after LIPUS therapy. Micro-CT, biomechanical testing, histologic evaluation, immunohistochemistry, quantitative realtime PCR, and Western blot were used for examination of the effects of LIPUS. Results Twelve weeks after LIPUS treatment, the loading strength in the control group was 355 ± 38 N (95% CI, 315-394 N), which was lower (p = 0.028) than that in the LIPUS group (441 ± 78 N; 95% CI, 359-524 N). The bone tissue volume density (bone volume/total volume) in the LIPUS group (49.29% ± 12.37%; 95 % CI, 36.31%-62.27%) was higher (p = 0.022) than that in the control group (37.93% ± 8.37%; 95 % CI, 29.15%-46.72%). The percentage of empty osteocyte lacunae in the LIPUS group (17% ± 4%; 95% CI, 15%-20%) was lower (p = 0.002) than that in the control group (26% ± 9%; 95% CI, 21%-32%). The mineral apposition rate (lm/day) in the LIPUS group (2.3 ± 0.8 lm/day; 95% CI, 1.8 2.8 lm/day) was higher (p = 0.001) than that in the control group (1.6 ± 0.3 lm/day; 95% CL, 1.4-1.8 lm/day). The number of blood vessels in the LIPUS group (7.8 ± 3.6/mm 2 ; 95% CI, 5.5-10.1 mm 2 ) was greater (p = 0.025) than the number in Hanxiao Zhu and Xunzi Cai contributed equally to this work and should be considered as co-first authors.The institution of the authors has received funding from the National Natural Science Foundation of China (Hangzhou, Zhejiang, China) (81101377, 81101345, 81171687, 81371954, 81201414, 81201416, 81401785, 81472113) 123Clin Orthop Relat Res (2015) 473:1830-1839 DOI 10.1007 Clinical Orthopaedics and Related Research ® A Publication of The Association of Bone and Joint Surgeons® the control group (5.7 ± 2.6/mm 2 ; 95% CI, 4.0-7.3 mm 2 ). Messenger RNA (mRNA) and protein expression of BMP-2 in the LIPUS group (75 ± 7, 95% CI, 70-7...

show abstract

Section: Discussioncontrasting

confidence: 80%

Section: Bmp-2 and Vegf Expressionmentioning

confidence: 90%

Section: Bmp-2 and Vegf Expressionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Low-intensity Pulsed Ultrasound Enhances Bone Repair in a Rabbit Model of Steroid-associated Osteonecrosis

Zhu

Cai

Lin

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

“…In the present state, it cannot be totally excluded that Bartonella infections of host cells induce a mitogenic activation or lead to a stress response of epithelial cells, giving rise to a secondary production of vasculoproliferative cytokines. Unspecific stimuli such as ultrasound have been described as potent inductors of IL-8 and VEGF production in various cell types (Reher et al, 1999). It has been discussed that ultrasound-triggered VEGF production might be an unspecific process linked to mechanical alterations in the cytoskeleton.…”

Section: Discussionmentioning

confidence: 99%

Evidence of a leading role for VEGF inBartonella henselae-induced endothelial cell proliferations

Kempf

Volkmann

Schaller

et al. 2001

Cellular Microbiology

150

184

View full text Add to dashboard Cite

Neuropilin-1 expression in osteogenic cells: Down-regulation during differentiation of osteoblasts into osteocytes

2001

View full text Add to dashboard Cite

The expression of neuropilin-1 (NRP1), a recently described VEGF and semaphorin receptor expressed by endothelial cells (EC) but some non-EC types as well, was analyzed in osteoblasts in vitro and in vivo. Cultured MC3T3-E1 osteoblasts expressed NRP1 mRNA and bound VEGF(165) but not VEGF(121), characteristic of the VEGF isoform-specific binding of NRP1. These cells did not express VEGFR-1 or VEGFR-2 so that VEGF binding to osteoblasts was strictly NRP1-dependent. In a chick osteocyte differentiation system, NRP1 was expressed by osteoblasts but its expression was absent as the cells matured into osteocytes. Immunohistochemical localization of NRP1 within the developing bones of 36-day-old mice and embryonic Day 17 chicks demonstrated that NRP1 was expressed by osteoblasts migrating alongside invading blood vessels within the metaphysis of the growth plate, as well as by osteoblasts at the developing edge of trabeculae within the marrow cavity. On the other hand, NRP1 was not expressed by osteocytes in either species, consistent with the in vitro results. In addition to osteogenic cells, NRP1 expression by EC was observed throughout the bone. Together these results suggest that NRP1 might have a dual function in bone by mediating osteoblast function directly as well as angiogenesis.

show abstract

Effect of Ultrasound on the Production of Il-8, Basic FGF and Vegf

Cited by 219 publications

References 0 publications

Low-intensity Pulsed Ultrasound Enhances Bone Repair in a Rabbit Model of Steroid-associated Osteonecrosis

Low-intensity Pulsed Ultrasound Enhances Bone Repair in a Rabbit Model of Steroid-associated Osteonecrosis

Evidence of a leading role for VEGF inBartonella henselae-induced endothelial cell proliferations

Neuropilin-1 expression in osteogenic cells: Down-regulation during differentiation of osteoblasts into osteocytes

Contact Info

Product

Resources

About