Effect of Turmeric, Turmerin and Curcumin on H2O2-Induced Renal Epithelial (LLC-PK1) Cell Injury

Cohly, Hari H. P.; Taylor, Annelle; Angel, Michael; Salahudeen, Abdulla K.

doi:10.1016/s0891-5849(97)00140-8

Cited by 118 publications

(60 citation statements)

References 21 publications

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“…Cultured human HSCs were exposed to different concentrations of curcumin for 24 h, and accumulation of TIMP-1 in cell-conditioned medium was assayed by ELISA. Concentrations of curcumin previously shown to modulate the biology of different cell types 25,26 resulted in a dose-dependent reduction of TIMP-1 secretion. Further increases in curcumin concentration did not result in more evident effects.…”

Section: Ementioning

confidence: 92%

Curcumin limits the fibrogenic evolution of experimental steatohepatitis

Vizzutti

Provenzano

Galastri

et al. 2010

Laboratory Investigation

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Nonalcoholic steatohepatitis is characterized by the association of steatosis with hepatic cell injury, lobular inflammation and fibrosis. Curcumin is known for its antioxidant, anti-inflammatory and antifibrotic properties. The aim of this study was to test whether the administration of curcumin limits fibrogenic evolution in a murine model of nonalcoholic steatohepatitis. Male C57BL/6 mice were divided into four groups and fed a diet deficient in methionine and choline (MCD) or the same diet supplemented with methionine and choline for as long as 10 weeks. Curcumin (25 mg per mouse) or its vehicle (DMSO) was administered intraperitoneally every other day. Fibrosis was assessed by Sirius red staining and histomorphometry. Intrahepatic gene expression was measured by quantitative PCR. Hepatic oxidative stress was evaluated by staining for 8-OH deoxyguanosine. Myofibroblastic hepatic stellate cells (HSCs) were isolated from normal human liver tissue. The increase in serum ALT caused by the MCD diet was significantly reduced by curcumin after 4 weeks. Administration of the MCD diet was associated with histological steatosis and necro-inflammation, and this latter was significantly reduced in mice receiving curcumin. Curcumin also inhibited the generation of hepatic oxidative stress. Fibrosis was evident after 8 or 10 weeks of MCD diet and was also significantly reduced by curcumin. Curcumin decreased the intrahepatic gene expression of monocyte chemoattractant protein-1, CD11b, procollagen type I and tissue inhibitor of metalloprotease (TIMP)-1, together with protein levels of a-smooth muscle-actin, a marker of fibrogenic cells. In addition, curcumin reduced the generation of reactive oxygen species in cultured HSCs and inhibited the secretion of TIMP-1 both in basal conditions and after the induction of oxidative stress. In conclusion, curcumin administration effectively limits the development and progression of fibrosis in mice with experimental steatohepatitis, and reduces TIMP-1 secretion and oxidative stress in cultured stellate cells.

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Section: Ementioning

confidence: 92%

Curcumin limits the fibrogenic evolution of experimental steatohepatitis

Vizzutti

Provenzano

Galastri

et al. 2010

Laboratory Investigation

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“…Curcumin has many biological effects, including antiinflammatory, antioxidant, anticarcinogenic, antiviral and anti-infectious activity (Joe et al, 2004). It protects against H 2 O 2 -induced oxidative stress in renal epithelial cells (Cohly et al, 1998) and inhibits the production of superoxide radicals, H 2 O 2 and NO by macrophages (Joe & Lokesh, 1994;van Meeteren et al, 2004) and astrocytes (Soliman & Mazzio, 1998). Although antioxidative effects cannot be excluded, the inhibitory effect of curcumin on clinical and pathological symptoms in murine EAE were attributed to blocking of IL-12 signalling through the Janus kinase-STAT pathway in T cells (Natarajan & Bright, 2002).…”

Section: Thiol-based Antioxidantsmentioning

confidence: 99%

Antioxidants and polyunsaturated fatty acids in multiple sclerosis

Meeteren¹,

Teunissen

Dijkstra

et al. 2005

Eur J Clin Nutr

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“…Nishinaka et al have reported that curcumin acts as a potent antioxidant [24]. It has also been previously reported that curcumin has a protective role against adriamycin-induced renal injury [25] and ferric nitrilotriacetateinduced oxidative renal damage in rats [26]. Thus, the preventive effect of curcumin on the MC-LR-induced reduction in the activity of GSH-Px, CAT and SOD could contribute to the restoration of markers of renal tubular injury.…”

Section: Discussionmentioning

confidence: 88%