Effect of triazole pesticide formulation on bovine culture cells

Holečková, Beáta; Šiviková, Katarı́na; Dianovský, Ján; Galdíková, Martina

doi:10.1080/03601234.2013.824224

Cited by 20 publications

(9 citation statements)

References 34 publications

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“…Based on this, we assume that epoxiconazole can reduce the number of spermatids and germ cells and result in abnormalities of germ cell development. There is no physiological germ cell apoptosis to maintain the male germline during germ cell development [36,37]. Therefore we focused on the number of meiotic cells in the adult male nematode and the meiosis entering time to illustrate germ cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis

Zhang

et al. 2016

IJERPH

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There is increasing evidence that epoxiconazole exposure can affect reproductive function, but few studies have investigated adverse effects on spermatogenesis. The nematode Caenorhabditis elegans (C. elegans) was used in our study to assess effects of epoxiconazole on spermatogenesis in male nematodes after 48 h of exposure to concentrations of 0.1, 1.0, or 10.0 μg/L. The results demonstrated that epoxiconazole exposure affected spermatogenesis, decreasing the number of total germ cells, mitotic cells, meiotic cells and spermatids, spermatid diameter, and cross-sectional area, and inducing mitotic germ cell proliferation arrest, premature entry into meiosis, and sperm activation inhibition; however, sperm transfer showed no abnormal changes. In addition, the results showed that epoxiconazole activated the transforming growth factor-β (TGFβ) signaling pathway and increased the expression levels of gene daf-1, daf-3, daf-4, daf-5 and daf-7 in nematodes. We therefore propose that epoxiconazole acts by activating the TGFβ signaling pathway, leading to the impairment of spermatogenesis and the consequent decline in male fertility.

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Section: Discussionmentioning

confidence: 99%

The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis

Zhang

et al. 2016

IJERPH

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“…Polymer inclusion membranes were prepared as reported in the earlier paper [ 34 , 35 , 36 , 37 , 40 , 45 ]. The membrane contained 2.7 cm 3 o-NPPE/1 g CTA, and 0.5–1.5 mol/dm 3 of 1-alkyltriazole 1 – 9 ( Table 1 ) based on a plasticizer.…”

Section: Methodsmentioning

confidence: 99%

“…Transport experiments were carried out in the system described in earlier papers [34][35][36][37]40,45] at 20 ± 0.2 • C. The feed phase was an aqueous solution of metal ions with a concentration of C 0,M = 0.001 mol/dm 3 each. The feed phase pH was kept constant (pH = 4.0).…”

Section: Transport Studiesmentioning

confidence: 99%

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New Polymer Inclusion Membranes in the Separation of Palladium, Zinc and Nickel Ions from Aqueous Solutions

2021

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The new polymer inclusion membrane (PIM) with a 1-alkyltriazole matrix was used to separate palladium(II) ions from aqueous chloride solutions containing a mixture of Zn-Pd-Ni ions. The effective conditions for transport studies by PIMs were determined based on solvent extraction (SX) studies. Furthermore, the values of the stability constants and partition coefficients of M(II)-alkyltriazole complexes were determined. The values of both constants increase with the growing hydrophobicity of the 1-alkyltriazole molecule and have the highest values for the Pd(II) complexes. The initial fluxes, selectivity coefficients, and recovery factors values of for Pd, Zn and Ni were determined on the basis of membrane transport studies. The transport selectivity of PIMs were: Pd(II) > Zn(II) > Ni(II). The initial metal ion fluxes for all the cations increased with the elongation of the alkyl chain in the 1-alkyltriazole, but the selectivity coefficients decreased. The highest values of the initial fluxes at pH = 4.0 were found for Pd(II) ions. The best selectivity coefficients Pd(II)/Zn(II) and Pd(II)/Ni(II) equal to 4.0 and 13.4, respectively, were found for 1-pentyl-triazole. It was shown that the microstructure of the polymer membrane surface influences the kinetics of metal ion transport. Based on the conducted research, it was shown that the new PIMs with 1-alkyltriazole can be successfully used in an acidic medium to separate a mixture containing Pd(II), Zn(II) and Ni(II) ions.

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“…Yu and co-workers [9] have observed that exposure to TB can alter thyroid hormone levels as well as gene transcription in zebrafish larvae. The results obtained by Holečková et al [10] indicated that TB can exert either cytotoxic and/or genotoxic effect on bovine peripheral lymphocytes. TB has been also found in farm workers with maximum concentrations of 19.2 μg/L and 2.22 ng/kg in urine and hair samples, respectively [11,12].…”

Section: Introductionmentioning

confidence: 99%

Fungicide Tebuconazole Influences the Structure of Human Serum Albumin Molecule

et al. 2019

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Studies of interactions between pesticides and target mammalian proteins are important steps toward understanding the pesticide′s toxicity. Using calorimetric and spectroscopic methods, the interaction between triazole fungicide tebuconazole and human serum albumin has been investigated. The spectroscopic techniques showed that fluorescence quenching of human serum albumin by tebuconazole was the result of the formation of tebuconazole/human serum albumin complex with the static type as the dominant mechanism. The association constant was found to be 8.51 × 103 L/mol. The thermodynamic parameters were obtained as ΔH = −56.964 kJ/mol, ΔS = −115.98 J/mol·K. The main active interactions forming the tebuconazole/human serum albumin complex were identified as the interplay between hydrogen bonds and/or van der Waals forces, based on thermodynamic experiments. These binding modes were corroborated well by the predictions of molecular modeling. Hydrogen bonding of tebuconazole with Arg222, Ala215 and Ala291 of human serum albumin played a relevant role in binding. The conformation changes in secondary structure were characterized by circular dichroism and 3D fluorescence spectra.

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Effect of triazole pesticide formulation on bovine culture cells

Cited by 20 publications

References 34 publications

The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis

The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis

New Polymer Inclusion Membranes in the Separation of Palladium, Zinc and Nickel Ions from Aqueous Solutions

Fungicide Tebuconazole Influences the Structure of Human Serum Albumin Molecule

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