Effect of trapping method on species identification of phlebotomine sandflies by MALDI‐TOF MS protein profiling

Halada, Petr; Hlavačková, Kristýna; Risueño, José María Bermúdez de Castro; Berriatua, E.; Volf, Petr; Dvořák, Vít

doi:10.1111/mve.12305

Cited by 23 publications

(16 citation statements)

References 16 publications

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“…Additional trapping methods must also be considered, since light traps have sampling limitations [31] and multi-trapping approaches have been demonstrated to promote more representative sampling of the local species community [6]. Nevertheless, a previous study [16] showed that the sampling method must be taken into consideration because it can considerably impact the quality of proteic spectra, especially when using sticky traps. When compared with CDC light traps, the quality of the spectra obtained from sand fly specimens collected by sticky traps was always lower and did not allow correct identification.…”

Section: Discussionmentioning

confidence: 99%

Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library

et al. 2019

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Phlebotomine sand flies are insects that are highly relevant in medicine, particularly as the sole proven vectors of leishmaniasis. Accurate identification of sand fly species is an essential prerequisite for eco-epidemiological studies aiming to better understand the disease. Traditional morphological identification is painstaking and time-consuming, and molecular methods for extensive screening remain expensive. Recent studies have shown that matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for rapid and cost-effective identification of arthropod vectors, including sand flies. The aim of this study was to validate the use of MALDI-TOF MS for the identification of Northern Amazonian sand flies. We constituted a MALDI-TOF MS reference database comprising 29 species of sand flies that were field-collected in French Guiana, which are expected to cover many of the more common species of the Northern Amazonian region, including known vectors of leishmaniasis. Carrying out a blind test, all the sand flies tested ( n = 157) with a log (score) threshold greater than 1.7 were correctly identified at the species level. We confirmed that MALDI-TOF MS protein profiling is a useful tool for the study of sand flies, including neotropical species, known for their great diversity. An application that includes the spectra generated here will be available to the scientific community in the near future via an online platform.

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Section: Discussionmentioning

confidence: 99%

Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library

et al. 2019

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“…Although this technique is promising for mosquitoes’ identification, some parameters have to be considered prior to MALDI-TOF MS analysis. Indeed, previous studies on several arthropods have underlined the impact of trap type used for sampling, the preservation methods and the body part used for protein extraction on the spectra profile [ 23 – 26 ]. The impact of trapping duration on MALDI-TOF identification, however, has never been evaluated.…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF MS: optimization for future uses in entomological surveillance and identification of mosquitoes from New Caledonia

et al. 2020

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Background: Mosquito vectors cause a significant human public health burden through the transmission of pathogens. Due to the expansion of international travel and trade, the dispersal of these mosquito vectors and the pathogens they carry is on the rise. Entomological surveillance is therefore required which relies on accurate mosquito species identification. This study aimed to optimize the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for mosquito identification. Methods: Aedes aegypti of the Bora-Bora strain and 11 field-sampled mosquito species were used in this study. Analyses were performed to study the impact of the trapping duration on mosquito identification with MALDI-TOF MS. The best preservation methods to use for short, medium and long-term preservation before MALDI-TOF MS analysis were also assessed. In addition, the number of specimens per species required for MALDI-TOF MS database creation was determined. The first MALDI-TOF database of New Caledonian mosquitoes was assembled and the optimal threshold for mosquito species identification according to the sensitivity and specificity of this technique was determined. Results: This study showed that the identification scores decreased as the trapping duration increased. High identification scores were obtained for mosquitoes preserved on silica gel and cotton at room temperature and those frozen at − 20 °C, even after two months of preservation. In addition, the results showed that the scores increased according to the number of main spectrum patterns (MSPs) used until they reached a plateau at 5 MSPs for Ae. aegypti. Mosquitoes (n = 67) belonging to 11 species were used to create the MALDI-TOF reference database. During blind test analysis, 96% of mosquitoes tested (n = 224) were correctly identified. Finally, based on MALDI-TOF MS sensitivity and specificity, the threshold value of 1.8 was retained for a secure identification score. Conclusions: MALDI-TOF MS allows accurate species identification with high sensitivity and specificity and is a promising tool in public health for mosquito vector surveillance.

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“…This probably means that a larger database may be needed to identify blood meals from several mosquito species. However, an increasing number of laboratories (Raharimalala et al, 2017;Halada et al, 2018;Mewara et al, 2018;Arfuso et al, 2019;Chavy et al, 2019;Rothen et al, 2016;Sambou et al, 2015;Yssouf et al, 2016) are applying MALDI-TOF to MS medical entomology and Raharimalala et al have also highlighted the need for sharing and have expressed interest in an international database (Raharimalala et al, 2017). Many reference spectra are also available on request or directly online (Lawrence et al, 2019), substantially facilitating database creation.…”

Section: Discussionmentioning

confidence: 99%

“…It has revolutionized clinical microbiology by making it possible to rapidly identify bacteria at a low cost (Seng et al ., 2009, 2013; Schubert and Kostrzewa, 2017). In recent years, the effectiveness of MALDI-TOF MS for identifying arthropods (Tandina et al , 2016, 2018a; Yssouf et al ., 2016; Diarra et al ., 2017; Laroche et al ., 2017 b ; Halada et al ., 2018; Mewara et al ., 2018) and mosquito blood meals identification (Niare et al 2016, 2017 b ; Tandina et al ., 2018 a , b ) has been reported. Indeed, in preliminary studies, MALDI-TOF MS was able to identify the host blood source from An.…”

Section: Introductionmentioning

confidence: 99%

Identification of mixed and successive blood meals of mosquitoes using MALDI-TOF MS protein profiling

et al. 2020

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BackgroundThe accurate and rapid identification of mosquito blood meals is critical to study the interactions between vectors and vertebrate hosts and, subsequently, to develop vector control strategies. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has been shown to be a reliable and effective tool for identifying single blood meals from mosquitoes.MethodsIn this study, we developed MALDI-TOF MS profiling protocols to identify Anopheles gambiae Giles, Anopheles coluzzii and Aedes albopictus mosquitoes’ mixed blood meals and the last of successive blood meals. The mosquitoes were either successively artificially fed with distinct host bloods or engorged with mixed bloods from distinct vertebrate hosts, such as humans, sheep and dogs.ResultsBlind test analyses revealed a correct identification of mixed blood meals from mosquitoes using MALDI-TOF MS profiling. The 353 MS spectra from mixed blood meals were identified using log score values >1.8. All MS spectra (n = 244) obtained from mosquitoes' successive blood meals were reproducible and specific to the last blood meal, suggesting that the previous blood meals do not have an impact on the identification of the last one.ConclusionMALDI-TOF MS profiling approach appears to be an effective and robust technique to identify the last and mixed blood meals during medical entomological surveys.

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Effect of trapping method on species identification of phlebotomine sandflies by MALDI‐TOF MS protein profiling

Cited by 23 publications

References 16 publications

Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library

Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library

MALDI-TOF MS: optimization for future uses in entomological surveillance and identification of mosquitoes from New Caledonia

Identification of mixed and successive blood meals of mosquitoes using MALDI-TOF MS protein profiling

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