2015
DOI: 10.1007/s10616-015-9918-1 View full text |Buy / Rent full text
|
|

Abstract: In this study, we evaluated three cell retention devices, an alternating tangential flow (ATF) system, a spin-filter, and a Centritech Lab III centrifuge, for the production of recombinant human Factor VIII co-expressed with von Willebrand factor. From the results, it was found that the FVIII activity in bioreactor was significantly higher in the ATF perfusion culture than two other perfusion cultures. Moreover, the FVIII activity yield was unexpectedly low in the ATF perfusion culture. We have, therefore, stu… Show more

Help me understand this report

Search citation statements

Order By: Relevance
Select...
3
1
0
5
0

Year Published

2018
2018
2019
2019

Publication Types

Select...
4

Relationship

0
4

Authors

Journals

0
5
0
Order By: Relevance
“…As evidenced by no increase in high molecular weight species, increased bioreactor residence time due to the decaying antibody sieving coefficient did not lead to a significant difference on the antibody critical quality attributes. Nonetheless, even though no product quality impact was observed for the antibody studied, other more liable molecules may be impacted (S. C. Kim et al, ).…”
Section: Resultsmentioning
Create an account to read the remaining citation statements from this report. You will also get access to:
  • Search over 1.2b+ citation statments to see what is being said about any topic in the research literature
  • Advanced Search to find publications that support or contrast your research
  • Citation reports and visualizations to easily see what publications are saying about each other
  • Browser extension to see Smart Citations wherever you read research
  • Dashboards to evaluate and keep track of groups of publications
  • Alerts to stay on top of citations as they happen
  • Automated reference checks to make sure you are citing reliable research in your manuscripts
  • 7 day free preview of our premium features.

Trusted by researchers and organizations around the world

Over 130,000 students researchers, and industry experts at use scite

See what students are saying

rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…As evidenced by no increase in high molecular weight species, increased bioreactor residence time due to the decaying antibody sieving coefficient did not lead to a significant difference on the antibody critical quality attributes. Nonetheless, even though no product quality impact was observed for the antibody studied, other more liable molecules may be impacted (S. C. Kim et al, ).…”
Section: Resultsmentioning
“…The cell density ceiling of a culture is, of course, linked to the particular cell separation technology used to enable perfusion. Most approaches relying on density‐based separation (gravitational, centrifugal, and acoustic settling, for example) have been typically demonstrated only to 20–30 million cells/ml (Dalm, Cuijten, van Grunsven, Tramper, & Martens, ; Kim et al, ). Early filtration approaches, such as spin filtration, are typically limited to peak densities around 30 million cells/ml, and filter fouling is a limiting factor (Kim et al, ; Patil & Walther, ).…”
Section: Introductionmentioning
“…These methods rely on a common principle to enable filtration even at high densities: Large tangential flows through a hollow‐fiber filter prevent cells and debris from accumulating and fouling the filter, while a much smaller cross flow is pulled through the fiber and sent downstream as harvest. Peak densities of over 100 million cells/ml have been demonstrated (Clincke et al, ; Kim et al, ), and cultures above 40 million cells/ml have been sustained for long durations (Karst, Serra, Villiger, Soos, & Morbidelli, ; Martin et al, ; Warikoo et al, ; Xu & Chen, ). If additional intensification continues to occur and these high‐density approaches prove translatable to commercial manufacturing, perfusion could become economically justifiable even for stable molecules traditionally manufactured with fed‐batch bioreactors.…”
Section: Introductionmentioning
“…Production processes for recombinant FVIII (both full-length or B domain-deleted) are all based on expensive perfusion technology in stainless steel fermenters [6][7][8]. The use of continuous perfusion technology allows the growth of FVIII-producing cells to high concentrations while ensuring a relatively short protein residence time [9,10]. Shorter residence times were proposed to allow efficient production and less disruption of the recombinant FVIII (rFVIII) protein [11], thus minimizing the generation of potentially immunogenic FVIII degradation products.…”
Section: Introductionmentioning