Effect of Topical Vitamin D Analogue on In Vivo Contact Sensitization

Hanneman, Kaija K.; Scull, Heather M.; Cooper, Kevin D.; Baron, Elma D.

doi:10.1001/archderm.142.10.1332

Cited by 31 publications

(27 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When hapten was applied to the 1,25(OH) 2 D 3 -treated sites in mice (using a local model of CHS), both no effect (12,13) and also down-regulation (14) of CHS responses have been reported. In a recent study using human subjects, calcipotriene, a 1,25(OH) 2 D 3 analog, significantly suppressed CHS responses by 64% (15). In this paper, increased regulatory activity of CD4 ϩ CD25 ϩ Foxp3 ϩ cells in the skin-draining lymph nodes (SDLN) following a single topical application of 1,25(OH) 2 D 3 is described.…”

mentioning

confidence: 85%

Topically Applied 1,25-Dihydroxyvitamin D3 Enhances the Suppressive Activity of CD4+CD25+ Cells in the Draining Lymph Nodes

Gorman

Kuritzky

Judge

et al. 2007

The Journal of Immunology

238

206

View full text Add to dashboard Cite

The immunomodulatory effects of vitamin D have been described following chronic oral administration to mice or supplementation of cell cultures with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the active form of vitamin D. In this study, topically applied 1,25(OH)2D3, enhanced the suppressive capacity of CD4+CD25+ cells from the draining lymph nodes. The effects of topical 1,25(OH)2D3 were compared with those of UVB irradiation, which is the environmental factor required for 1,25(OH)2D3 production in skin. CD4+ cells from the skin-draining lymph nodes (SDLN) of either 1,25(OH)2D3-treated or UVB-irradiated mice had reduced capacity to proliferate to Ags presented in vitro, and could suppress Ag-specific immune responses upon adoptive transfer into naive mice. This regulation was lost upon removal of CD4+CD25+ cells. Furthermore, purified CD4+CD25+ cells from the SDLN of 1,25(OH)2D3-treated or UVB-irradiated mice compared with equal numbers of CD4+CD25+ cells from control mice had increased capacity to suppress immune responses in both in vitro and in vivo assay systems. Following the sensitization of recipient mice with OVA, the proportion of CD4+Foxp3+ cells of donor origin significantly increased in recipients of CD4+CD25+ cells from the SDLN of 1,25(OH)2D3-treated mice, indicating that these regulatory T cells can expand in vivo with antigenic stimulation. These studies suggest that 1,25(OH)2D3 may be an important mediator by which UVB-irradiation exerts some of its immunomodulatory effects.

show abstract

mentioning

confidence: 85%

Topically Applied 1,25-Dihydroxyvitamin D3 Enhances the Suppressive Activity of CD4+CD25+ Cells in the Draining Lymph Nodes

Gorman

Kuritzky

Judge

et al. 2007

The Journal of Immunology

238

206

View full text Add to dashboard Cite

show abstract

“…Skin exposure to calcipotriol (three times daily for 3 days (1500 ng/treatment)) before transcutaneous immunization with ovalbumin (OVA) protein also reduced CHS ear-swelling responses in mice [6]. In addition, human volunteers treated topically with the vitamin D analogue calcipotriene twice-daily had significantly suppressed CHS responses when the hapten (DNCB, di-nitrochlorobenzene) was applied to the calcipotriene-treated site [11]. Similarly, Mantoux reactions were suppressed by topical administration of 1,25(OH) 2 D 3 to the skin of human volunteers [12].…”

Section: Regulation Of Skin-derived Dendritic Cells By Topical Vitamin Dmentioning

confidence: 99%

“…Further, the ability of 1,25(OH) 2 D 3 to reverse UVB-induced immunosuppression in Skh:hr1 mice may occur through the non-genomic "rapid-response" pathway and not through one dependent on the genomic VDR [13]. The presence of the VDR in the skin of BALB/c mice and humans [11], where calcipotriol also suppresses CHS responses, may thus enable some of the suppressive actions of topical 1,25(OH) 2 D 3 . In addition, in recent studies, 1,25(OH) 2 D 3 application immediately following UV irradiation did not reverse the immunosuppressive effects of UV irradiation on Mantoux reactions [12].…”

Section: Regulation Of Skin-derived Dendritic Cells By Topical Vitamin Dmentioning

confidence: 99%

Immune-modifying properties of topical vitamin D: Focus on dendritic cells and T cells

Gorman

Judge

Hart

2010

The Journal of Steroid Biochemistry and Molecular Biology

View full text Add to dashboard Cite

“…24 Interestingly, high levels of vitamin D receptor signalling can also cause immunosuppression in human skin. 25 This suggests that excessively high levels of serum 25-hydroxyvitamin D may in fact add to the tissue's burden of immunosuppressive events that occur after UV injury, and further diminution of the tumor immune surveillance capacity of the skin's immune system.…”

Section: Effects Of Ultraviolet Radiationmentioning

confidence: 99%