Effect of Thermal and Cycloheximide Pre‐treatments on Light‐Dependent Necrosis Formation to Rice Leaves by<i>Pyricularia oryzae</i> Toxin(s)*

Arase, Seiji; Nozu, Mikio; Iedome, M.; Honda, Yuichi; Yamaji, Yohei

doi:10.1111/j.1439-0434.1993.tb01366.x

Cited by 6 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, when the mutant‐type response was induced in the rice sl ‐mutant, the resistance to M. grisea was greater than that in the rice with the wild‐type response (Arase et al ., 2000a,b). Sekiguchi lesion formation was also induced by a spore germination fluid (SGF) of M. grisea (Figure 1) and significantly inhibited by pre‐treatment with cycloheximide (Arase et al ., 1993, 1997). These results suggested that the induction of Sekiguchi lesions is a marker for defense system activation in sl ‐mutants, but not the one for passive susceptible reaction.…”

Section: Introductionmentioning

confidence: 99%

Increased tryptophan decarboxylase and monoamine oxidase activities induce Sekiguchi lesion formation in rice infected with Magnaporthe grisea

et al. 2003

View full text Add to dashboard Cite

SummarySekiguchi lesion (sl)-mutant rice infected with Magnaporthe grisea showed increased light-dependent tryptophan decarboxylase (TDC) and monoamine oxidase (MAO) activities. TDC and MAO activities were observed before the penetration of M. grisea to rice cells and maintained high levels even after Sekiguchi lesion formation. Light-dependent expression of TDC gene was observed in leaves inoculated with M. grisea before Sekiguchi lesion formation. Spore germination¯uid (SGF) of M. grisea also induced Sekiguchi lesion formation accompanied by increased enzymes activities and tryptamine accumulation. Sekiguchi lesion was also induced by treatments with tryptamine and b-phenylethylamine, which are substrates for MAO, but was not induced by non-substrates such as indole-3-propionic acid, (AE)-phenylethylamine and tryptophan under light. Light-dependent induction of Sekiguchi lesion by tryptamine was signi®cantly inhibited in the presence of MAO inhibitors, metalaxyl and semicarbazide, and H 2 O 2 -scavengers, ascorbic acid and catalase. H 2 O 2 in M. grisea-infected leaves with and without Sekiguchi lesions was demonstrated directly in situ by strong 3,3 H -diaminobenzidine (DAB) staining. On the other hand, H 2 O 2 induced Sekiguchi lesions on leaves of cv. Sekiguchi-asahi under light, but not in darkness. This difference was associated with the decrease of catalase activity in infected leaves under light and the absence of decrease in darkness. We hypothesize that the H 2 O 2 -induced breakdown of cellular organelles such as chloroplasts and mitochondria in mesophyll cells may cause high TDC and MAO activities and the development of Sekiguchi lesion, and that the sl gene products in wild-type rice may function as a suppressor of organelle breakdown caused by chemical or environmental stress.

show abstract

Section: Introductionmentioning

confidence: 99%

Increased tryptophan decarboxylase and monoamine oxidase activities induce Sekiguchi lesion formation in rice infected with Magnaporthe grisea

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Tryptamine also accumulated in leaf necrosis induced by the spore germination¯uid under light, whereas it did not accumulate in the leaves where leaf necrosis formation was suppressed by dark treatment (data not shown). However, neither tryptamine accumulation nor leaf necrosis was induced in leaves that were pretreated with the protein-and photosynthetic inhibitors cycloheximide and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) (Arase et al, 1993;Iedome et al, 1995), respectively, even under light (data not shown). Furthermore, tryptamine (600 lg/ml) induced Sekiguchi lesion-like necrosis in leaves of the rice sl-mutant under light.…”

Section: Resultsmentioning

confidence: 82%

Light‐Dependent Accumulation of Tryptamine in the Rice Sekiguchi Lesion Mutant Infected with Magnaporthe grisea

Arase

Ueno

Toko

et al. 2001

Journal of Phytopathology

Self Cite

View full text Add to dashboard Cite

An antifungal compound with an infection-inhibiting activity was isolated from a rice Sekiguchi lesion (sl) mutant and identi®ed as the indole alkaloid tryptamine. Tryptamine inhibited not only spore germination and appressorium formation of Magnaporthe grisea at high concentrations (> 600 lg/ml) but also the infection hypha formation in onion cells at low concentrations (150±300 lg/ml). Tryptamine is a normal compound of the rice sl-mutant but accumulates further in rice with a mutant-type response (Sekiguchi lesion formation) to inoculation with M. grisea spores. A mutant type of response is involved in light-enhanced resistance. The accumulation of tryptamine was not induced, however, in rice with a wild-type response (blast lesion formation). This study strongly suggests that tryptamine plays an important role as a possible factor in light-enhanced resistance in the rice sl-mutants.

show abstract

“…Enhanced resistance composed of the above two components was affected by both visible light and rice leaf senescence. In previous studies ( Arase et al, 2000, 1993b; Iedome et al, 1995), it was reported that the most effective wavelength for inducing Sekiguchi lesion formation was from 400 to 700 nm, and that lesion formation was significantly inhibited by a photosynthetic inhibitor, DCMU (3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea), and a protein synthetic inhibitor, cycloheximide. Thus, the photo‐ and de novo protein‐syntheses may be involved in the expression of the enhanced resistance in the rice sl mutant.…”

Section: Resultsmentioning

confidence: 99%

Light‐Enhanced Resistance to Magnaporthe grisea Infection in the Rice Sekiguchi Lesion Mutants

Arase

Fujita

Uehara

et al. 2000

Journal of Phytopathology

Self Cite

View full text Add to dashboard Cite

The rice sl mutant showed two types of responses to Magnaporthe grisea infection by light treatments. One was an sl‐mutant‐type response characterized by Sekiguchi lesion expression under light waves of 400–700 nm, and the other was a wild‐type response characterized by blast and/or necrotic spot lesion expression in the dark or at wavelength between 290 and 330 nm. There was a large difference in the resistance to M. grisea infection between the mutant‐ and wild‐type responses in the rice sl mutant. When the mutant‐type response was induced in the rice sl mutant, the disease resistance was enhanced relative to that in the wild‐type response. Enhanced resistance was demonstrated by two components: (a) the number of Sekiguchi lesions was reduced relative to that of blast or necrotic lesions; (b) sporulation of M. grisea was not induced in Sekiguchi lesions. The enhanced resistance was dependent on light of 400–700 nm.

show abstract

Effect of Thermal and Cycloheximide Pre‐treatments on Light‐Dependent Necrosis Formation to Rice Leaves byPyricularia oryzae Toxin(s)*

Cited by 6 publications

References 9 publications

Increased tryptophan decarboxylase and monoamine oxidase activities induce Sekiguchi lesion formation in rice infected with Magnaporthe grisea

Increased tryptophan decarboxylase and monoamine oxidase activities induce Sekiguchi lesion formation in rice infected with Magnaporthe grisea

Light‐Dependent Accumulation of Tryptamine in the Rice Sekiguchi Lesion Mutant Infected with Magnaporthe grisea

Light‐Enhanced Resistance to Magnaporthe grisea Infection in the Rice Sekiguchi Lesion Mutants

Contact Info

Product

Resources

About