Effect of the combination of several irrigants on dentine surface properties, adsorption of chlorhexidine and adhesion of microorganisms to dentine

Tartari, Talita; Wichnieski, Caroline; Bachmann, Luciano; Jafelicci, Miguel; Silva, R. M.; Letra, Ariadne; Hoeven, Ransome van der; Duarte, Marco Antônio Húngaro; Bramante, Clóvis Monteiro

doi:10.1111/iej.12960

Cited by 22 publications

(30 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…And wettability of surfaces is related to several factors, such as roughness, hydration state of the solid surface and the properties of liquid contacted with substrate (Rosales et al 1999, Huang et al 2012). After the use of CHX as a final irrigant, reductions in the wettability of dentine surfaces were reported (Tartari et al 2018). However, treatment with amphipathic solutions significantly increased dentine wettability (Ricci et al 2014), GH12 was designed to be a positively charged amphipathic α‐helical AMP that contains optimal content of hydrophobic residues (40–60%) and relative amphipathicity (0.3–0.6) (Wang et al 2017), which might lead to a better wettability and thus deeper penetration into dentinal tubules than CHX.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial peptide GH12 as root canal irrigant inhibits biofilm and virulence of Enterococcus faecalis

Wang

Chen

et al. 2020

Int Endodontic J

View full text Add to dashboard Cite

Aim The objectives of this laboratory‐based study were to investigate the effects of GH12 on Enterococcus faecalis biofilm and virulence. Methodology Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of GH12 against E. faecalis were first determined. A time‐kill assay was further conducted. The effects of GH12 on the expression of virulence and stress genes in E. faecalis were evaluated by RT‐qPCR. Crystal violet stain was used to investigate the effects of GH12 on E. faecalis biofilm formation and 1‐day‐old biofilm. Finally, an ex vivo tooth model contaminated with E. faecalis was used to evaluate the antimicrobial activity of GH12 as an irrigant by CFU counting, SEM and CLSM. One‐way anova and Tukey’s multiple comparisons test were used to compare the differences amongst groups (α = 0.05). Results The MICs and MBCs of GH12 against E. faecalis were 8.0 ± 0.0 and 16.0 ± 0.0 mg L−1, respectively, and GH12 at 32.0 mg L−1 reduced the bacterial numbers by more than 99.9% within 1 min. Various virulence genes (efaA, esp and gelE) and stress genes (dnaK, groEL, ctsR and clpPBCEX) in E. faecalis were significantly downregulated by GH12 at sub‐MIC levels (P < 0.05). Additionally, both E. faecalis biofilm formation and the biomass of 1‐day‐old E. faecalis biofilm were significantly reduced by GH12 (P < 0.05). Elimination of E. faecalis in biofilms from root canal walls was achieved through irrigation with 64.0 mg L−1 GH12 for 30 min. CLSM analysis revealed that GH12 at 64.0 mg L−1 was most effective in eliminating bacteria within dentinal tubules (P < 0.05). Conclusion In a laboratory setting, and when used as an irrigant, GH12 suppressed E. faecalis, downregulated specific virulence and stress‐associated genes, eliminated intracanal E. faecalis protected by biofilms and killed bacteria in dentinal tubules. These results emphasize the need for preclinical and clinical studies to explore the potential of GH12 as an antimicrobial agent during root canal treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial peptide GH12 as root canal irrigant inhibits biofilm and virulence of Enterococcus faecalis

Wang

Chen

et al. 2020

Int Endodontic J

View full text Add to dashboard Cite

show abstract

“…Erosion, caused by irrigating solutions, has been widely evaluated in the literature with the use of SEM that allows a morphologic detailed evaluation of the prepared surface. 21 According to the literature, [20][21][22] depending on the depth, this erosion can be a contributing factor to the vertical root fracture but may contribute to the increasing rates of wallcleaning of the canal, removing debris and bacteria from the root canal. 22,23 This study used a mechanical agitation by ultrasound due to its acoustic transmission and formation of bubbles due to the phenomenon of cavitation, which explodes and increases the temperature and pressure, resulting in waves of impact against the wall of the canal by removing debris.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of the Chelating Solutions in the Resistance of Glass Fiber Posts to the Root Dentin

et al. 2020

View full text Add to dashboard Cite

Objective To evaluate the influence of chelating agents (EDTA, citric acid and Tetraclean) on glass fiber posts adhesion to root dentin. Materials and Methods Forty mandibular premolars single canals, with complete apical root, straight, circular cross sections were selected, accessed and had the cervical third prepared with Gates-Glidden drills, then included in resin, instrumented with ProTaper Universal and the root canal obturation was carried out. After, the samples were randomly divided into 4 groups (n = 10) to test the final irrigation solutions: G1:17% EDTA; G2:10% citric acid; G3: Tetraclean and G4: saline solution (control). After 30 days of storage (36.5° C and 100% humidity), the post preparation was performed leaving 4 mm of apical endodontic filling. Then, a fiber-glass post previously selected was coated with ED Primer adhesive system and resin sealer Panavia, installed and stored for 24 hours at 37°C. The samples were subjected to a tensile test with a constant speed of 1 mm/min with 2000 Kgf. The results were analyzed with the ANOVA test. Results The statistical analyzes indicated no significant differences between the groups (p > 0.05). Conclusion The type of chelating agent used in the final irrigation of the endodontic treatment did not influence the tensile strength of the fixation system used to sealer the glass fiber posts to the intracanal dentin.

show abstract

“…The sessile drop method was performed using the OCA-20 system (DataPhysics Instruments GmbH) and SCA-20 software (DataPhysics Instruments GmbH) to determine the contact angle (Ө). Three 5 μL aliquots of each solution were pipetted at 3 different points on the dentin surface, and the images were captured after 30 seconds until each drop was stable [24]. The average of the 3 values was considered the final value for each specimen (Figure 1).…”

Section: Contact Anglementioning

confidence: 99%

Physicochemical properties, cytotoxicity and penetration into dentinal tubules of sodium hypochlorite with and without surfactants

et al. 2020

View full text Add to dashboard Cite

Objectives: The aim of this study was to assess the physicochemical properties, cytotoxicity and penetration into dentinal tubules of ChlorCid™ Surf (3% sodium hypochlorite [NaOCl] with surfactant) in comparison to ChlorCid™ (3% NaOCl without surfactant). Materials and Methods: The physicochemical properties evaluated were pH, surface tension, free available chlorine (FAC) and contact angle. Cytotoxicity was evaluated in L929 fibroblasts exposed to the solutions by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and neutral red assays. Assessment of penetration into dentinal tubules was performed by staining single-rooted permanent human teeth with crystal violet (n = 9), which were irrigated with the solutions and analyzed in cervical, middle and apical segments. Data were analyzed by one-way analysis of variance (ANOVA) and Tukey's post-test, 2-way ANOVA and Bonferroni's post-test or t-test (α = 0.05). Results: ChlorCid™ Surf and ChlorCid™ FAC values were close to those indicated by the manufacturer. ChlorCid™ Surf showed lower surface tension and contact angle on dentin, and higher pH than ChlorCid™ (p < 0.05). The penetration of ChlorCid™ Surf was higher in cervical and middle segments, compared with ChlorCid™ (p < 0.05). There was no difference in irrigant cytotoxicity (p > 0.05). Conclusions: ChlorCid™ Surf showed lower surface tension, lower contact angle on root canal dentin, higher penetration into dentinal tubules and more alkaline pH, compared with ChlorCid™. However, both solutions showed similar cytotoxicity and FAC content.

show abstract

Effect of the combination of several irrigants on dentine surface properties, adsorption of chlorhexidine and adhesion of microorganisms to dentine

Abstract: The irrigation solutions had a variable effect on the properties of dentine, on the adhesion of E. faecalis and C. albicans and the adsorption of CHX to the dentine surface.

Cited by 22 publications

References 98 publications

Antimicrobial peptide GH12 as root canal irrigant inhibits biofilm and virulence of Enterococcus faecalis

Antimicrobial peptide GH12 as root canal irrigant inhibits biofilm and virulence of Enterococcus faecalis

Influence of the Chelating Solutions in the Resistance of Glass Fiber Posts to the Root Dentin

Physicochemical properties, cytotoxicity and penetration into dentinal tubules of sodium hypochlorite with and without surfactants

Contact Info

Product

Resources

About