Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues

Yamada, Tomoya; Higuchi, Mikito; Nakanishi, Naoto

doi:10.1016/j.bbrc.2015.06.036

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…In contrast, “premature senescence” is induced by stressors such as oxidative stress and ROS without telomere-independent mechanisms [ 51 , 57 ]. Our previous study indicated that telomere length was not associated with adipocyte hypertrophy from three anatomical sites (subcutaneous, visceral and intramuscular) in obese cattle [ 56 ]. These results suggest that obese visceral adipose tissue is in a state of premature senescence.…”

Section: Discussionmentioning

confidence: 99%

“…Pref-1 is a transmembrane protein acts as a molecular gatekeeper of adipogenesis [ 48 ]. Our previous reports showed that pref-1 gene expression level was fat-depot specific, and pref-1 expression level was positively correlated with the subcutaneous adipocyte size, and negatively correlated with the intramuscular and visceral adipocyte size [ 56 ]. These results suggest that p53 expression might be regulated negatively by the pref-1 pathway.…”

Section: Discussionmentioning

confidence: 99%

“…mRNA expression was analyzed by real-time PCR as described previously [ 54 , 55 , 56 ]. In brief, total RNA was extracted from adipose tissue using a RiboPure Kit (Ambion) in accordance with the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

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Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

Yamada

Kamiya

Higuchi

et al. 2018

The Journal of Veterinary Medical Science

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Obesity is associated with the chronic inflammation and senescence of adipose tissues. Macrophage is a key mediator of chronic inflammation that infiltrates obese adipose tissue and stimulates metabolic disorders. However, the fat depot-specific differences of macrophage infiltration and senescence, especially the influence on intramuscular adipose tissue, have remained unclear. We investigated the fat depot-specific differences of macrophage infiltration and senescence in obese bovine adipose tissue from three different anatomical sites (subcutaneous, intramuscular and visceral). Macrophage infiltrations and crown-like structures were observed in visceral adipose tissue, although there were few macrophages in subcutaneous and intramuscular adipose tissues. The positive reaction of senescence marker SA-βgal activity was observed in visceral adipose tissue. In contrast, the activity of SA-βgal in subcutaneous and intramuscular adipose tissues were low. The expression of p53 gene, the master regulator of cellular senescence, in visceral adipose tissue was higher than that of subcutaneous and intramuscular adipose tissue. At the cellular level, p53 gene expression was negatively correlated with the size of subcutaneous adipocytes. In contrast, p53 gene expressions were positively correlated with the size of intramuscular and visceral adipocytes. These results indicate that anatomical sites of obese adipose tissue affect macrophage infiltration and the senescent state in a fat depot-specific manner.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

Yamada

Kamiya

Higuchi

et al. 2018

The Journal of Veterinary Medical Science

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Soluble delta-like 1 homolog (DLK1) stimulates angiogenesis through Notch1/Akt/eNOS signaling in endothelial cells

Huang

Kuo

et al. 2018

Angiogenesis

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Expression of uncoupling protein 1 in bovine muscle cells1

Eldaim

Hashimoto

Ohtsuki

et al. 2016

Journal of Animal Science

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Uncoupling protein 1 (Ucp1) is predominantly expressed in brown/beige adipocytes in mammals. Although myogenic cells have been suggested to commit to a brown adipocyte lineage through the induction of Prdm16 expression, Prdm16 is also expressed in skeletal muscle. Thus, we examined expression of Ucp1 in bovine myogenic cells. Considering that Ucp1 is a principle molecule that induces energy expenditure in brown/beige adipocytes, expression of Ucp1 is not preferable in beef cattle because of potential decrease in energy (fattening) efficiency. The RT-PCR analyses revealed the expression of Ucp1 in the skeletal muscle of cattle; expression levels were markedly lower than those in the brown fat of calves. Immunohistochemical analyses showed that Ucp1 surrounded muscle fibers, but not adipocytes residing in skeletal muscle. Myosatellite cells cultured in myogenic medium showed an increase in the expression levels of myogenic regulatory factors ( < 0.05), while those in cells cultured in adipogenic medium were decreased ( < 0.05). The Ucp1 expression was also detected in myosatellite cells; expression levels were greater in cells after myogenic culture for 12 d than in those after myogenic culture for 6 d ( < 0.05) and were decreased when cells were cultured in adipogenic medium ( < 0.05). The Prdm16 expression was not affected by culture conditions, suggesting that the expression of Ucp1 is not regulated by that of Prdm16. The results of the present study provide an insight into the unexpected expression of Ucp1 in bovine skeletal muscle, which suggests the necessity for further studies on Ucp1-mediated energy expenditure in bovine skeletal muscle.

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Effect of the anatomical site on telomere length and pref-1 gene expression in bovine adipose tissues

Cited by 3 publications

References 27 publications

Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

Soluble delta-like 1 homolog (DLK1) stimulates angiogenesis through Notch1/Akt/eNOS signaling in endothelial cells

Expression of uncoupling protein 1 in bovine muscle cells1

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