Effect of tamoxifen treatment on the semen quality and fertility of the male rat

Motrich, Rubén Darío; Ponce, Andrés Alberto; Rivero, Virginia

doi:10.1016/j.fertnstert.2006.11.196

Cited by 26 publications

(19 citation statements)

References 56 publications

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“…The first lines to use this utilised CreER; however, this was found to be quite leaky, with recombination occurring in the absence of ligand, and was quickly superseded by the CreER T2 to provide higher specificity (Feil et al 2009). However, tamoxifen treatment can impact upon the architecture and function of the rodent testis (Fisher et al 1999, Motrich et al 2007. Unless adequately controlled for, such effects may severely curtail the ability to elucidate meaningful results from a study.…”

Section: Inducible Promotersmentioning

confidence: 99%

Good planning and serendipity: exploiting the Cre/Lox system in the testis

Smith¹

2011

Reproduction

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Over the past 20 years, genetic manipulation has revolutionised our understanding of male reproductive development and function. The advent of transgenic mouse lines has permitted elegant dissection of previously intractable issues. The development of the Cre/Lox system, which has permitted spatial and temporal localisation of genetic manipulation, has expanded upon this, and now makes up one of the primary approaches underpinning our increasing understanding of testis development and function. The success of conditional gene targeting is largely reliant upon the choice of Cre recombinase expressing mouse line, which is required to specifically target the correct cell type at the correct time. Presupposition that Cre lines will behave as expected has been one of the main oversights in the design of Cre/Lox experiments, as in practice, many Cre lines are prone to ectopic expression (both temporal and spatial), transgene silencing or genetic background effects. Empirical validation of the spatiotemporal profile of Cre expression prior to undertaking conditional gene targeting studies is essential and can be achieved through a combination of molecular and immunohistochemical approaches, along with in vivo examination of reporter gene expression in targeted tissues. This paper details the key considerations associated with exploitation of the Cre/Lox system and highlights a variety of validated Cre lines that have utility for conditional gene targeting within the testis.

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Section: Inducible Promotersmentioning

confidence: 99%

Good planning and serendipity: exploiting the Cre/Lox system in the testis

Smith¹

2011

Reproduction

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“…Treatment of mice with the antiestrogen fulvestrant (ICI 182,780) induced capping and vesiculation of narrow cells in the initial segment, accumulation of PAS-positive granules in apical cells of the caput of the epididymis, an increase in lysosomal granules in clear cells of the corpus and cauda, and a decrease in the concentration of cauda sperm, progressive sperm motility and a decre-ase in fertility (55). In the male rat, treatment with the nonsteroidal type I selective estrogen receptor modulator (SERM) tamoxifen induced alterations in testis and epididymis that seem to be responsible for a decrease in sperm concentration and motility (62). Estrogen may also be involved in the ejaculatory process, regulating the contractile activity of the rabbit epididymis during semen emission (63).…”

Section: Epididymismentioning

confidence: 99%

Estrogen receptors and function in the male reproductive system

Lazari

Lucas

Yasuhara

et al. 2009

Arq Bras Endocrinol Metab

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A substantial advance in our understanding on the estrogen signaling occurred in the last decade. Estrogens interact with two receptors, ESR1 and ESR2, also known as ERα and ERβ, respectively. ESR1 and ESR2 belong to the nuclear receptor family of transcription factors. In addition to the well established transcriptional effects, estrogens can mediate rapid signaling, triggered within seconds or minutes. These rapid effects can be mediated by ESRs or the G protein-coupled estrogen receptor GPER, also known as GPR30. The effects of estrogen on cell proliferation, differentiation and apoptosis are often mediated by growth factors. The understanding of the cross-talk between androgen, estrogen and growth factors signaling pathways is therefore essential to understand the physiopathological mechanisms of estrogen action. In this review we focused on recent discoveries about the nature of the estrogen receptors, and on the signaling and function of estrogen in the male reproductive system. Arq Bras Endocrinol Metab. 2009;53(8):923-33 Keywords Estrogens; receptors, estrogen; reproduction; male resumo Durante a última década, ocorreu um avanço substancial no conhecimento da sinalização do estrógeno. Estrógenos interagem com dois receptores, ESR1 e ESR2, também conhecidos como ERα e ERβ, respectivamente. ESR1 e ESR2 pertencem à família de receptores nucleares, que funcionam como fatores de transcrição. Além dos bem estabelecidos efeitos transcricionais, os estrógenos medeiam a sinalização rápida, desencadeada dentro de segundos ou minutos. Esses efeitos rápidos podem ser mediados por ESRs ou pelo receptor de estrógeno acoplado à proteína G, GPER, também conhecido como GPR30. Os efeitos de estrógenos sobre a proliferação celular, diferenciação e apoptose são, muitas vezes, mediados por fatores de crescimento. Portanto, a compreensão da interação entre as vias de sinalização de andrógeno, estrógeno e fatores de crescimento é essencial para entender os mecanismos fisiopatológicos envolvidos na ação estrogênica. Nesta revisão, foram abordadas descobertas recentes sobre a estrutura dos receptores, a sinalização e a função do estrógeno no sistema reprodutor masculino. Arq Bras Endocrinol Metab. 2009;53(8):923-33

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“…Fertility parameters were determined as previously described (23). The females mated with the control and autoimmune males were euthanized on day 21 of gestation.…”

Section: Fertility Parametersmentioning

confidence: 99%

“…AE 1 23. 32.31 AE 10.20 a a Significantly different with respect to group C (P< .03) by unpaired Student's t test.…”

mentioning

confidence: 98%