Effect of sustained hyperadrenalinemia on exercise performance and lactate threshold in rats

Langfort, Józef; Zarzeczny, Ryszard; Pilis, Wiesław; Kaciuba-Uściłko, H.; Nazar, K; Porta, S.

doi:10.1016/0300-9629(95)02087-x

Cited by 23 publications

(17 citation statements)

References 23 publications

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“…Afterwards, the animals were submitted to 3-minute physical effort stages, followed by 2-minute intervals for blood sampling. The initial speed was 13m.min , followed by increments of 4m.min -1 in each 3-minute stage, with constant inclination of 10%, until exhaustion, according to procedures suggested by Langfort et al (1996) and Pilis et al (1993). LT was visually identified by the blood lactate concentration versus workload curve, considering the workload corresponding to the inflection point of blood lactate concentration within a range of 2-4mM (Cunha et al, 2008(Cunha et al, , 2009 (Figure 1).…”

Section: Incremental Testmentioning

confidence: 99%

Increased lactate threshold after five weeks of treadmill aerobic training in rats

Vieira

Luís

2014

Braz. J. Biol.

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The aim of the present study was to analyse the lactate threshold (LT) changes in rats submitted to an aerobic treadmilltraining programme. Twenty-five Wistar rats were divided into two groups: a sedentary control group (CG), and a trained group (TG) submitted to an aerobic training during 5 weeks. All the animals were submitted to an incremental treadmill exercise test in order to determine LT. There was an increase in the maximum running speed in the TG (from 32.25 ± 1.27 to 47.75 ± 3.13 m.min -1 -p = 0.001), and running speed at LT (from 26.21 ± 1.15 to 35.30 ± 2.24 m.min-p = 0.004), a part from the reduction in blood lactate at LT. LT can be determined in rats, and aerobic training induced positive oxidative physiological adaptations in the animals.Keywords: anaerobic threshold, running speed, blood lactate, exercise. Aumento do limiar de lactato após cinco semanas de treinamento aeróbio em esteira em ratosResumo O objetivo do presente estudo foi analisar as mudanças no limiar de lactato (LL) em ratos submetidos a um programa de treinamento aeróbio em esteira. Vinte e cinco ratos Wistar foram divididos em dois grupos: um grupo controle sedentário (CG), e um grupo treinado (GT) submetido a um treinamento aeróbio durante 5 semanas. Todos os animais foram submetidos a um teste de exercício incremental em esteira, a fim de determinar o LL. Houve um aumento na velocidade máxima de corrida no GT (de 32,25 ± 1,27 para 47,75 ± 3,13 m.min -1 -p = 0,001), e velocidade de corrida no LL (de 26,21 ± 1,15 para 35,30 ± 2,24 m.min -1 -p = 0,004), além da redução na concentração de lactato no LL. O LL pode ser determinado em ratos e o treinamento aeróbio induziu a adaptações fisiológicas oxidativas positivas nos animais.Palavras-chave: limiar anaeróbio, velocidade de execução, lactato sanguíneo, exercício.

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Section: Incremental Testmentioning

confidence: 99%

Increased lactate threshold after five weeks of treadmill aerobic training in rats

Vieira

Luís

2014

Braz. J. Biol.

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“…However, this procedure is limited by the small amount of information available concerning lactate kinetics during exercise in rats. To our knowledge, only few studies dealing with lactate threshold determinations in rats have been reported in the literature, most of them using treadmill running as an ergometer (14,15). Only one study analyzed lactate kinetics during swimming (16).…”

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confidence: 99%

Determination of anaerobic threshold in rats using the lactate minimum test

Voltarelli

Gobatto

Mello

2002

Braz J Med Biol Res

149

124

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The break point of the curve of blood lactate vs exercise load has been called anaerobic threshold (AT) and is considered to be an important indicator of endurance exercise capacity in human subjects. There are few studies of AT determination in animals. We describe a protocol for AT determination by the "lactate minimum test" in rats during swimming exercise. The test is based on the premise that during an incremental exercise test, and after a bout of maximal exercise, blood lactate decreases to a minimum and then increases again. This minimum value indicates the intensity of the AT. Adult male (90 days) Wistar rats adapted to swimming for 2 weeks were used. The initial state of lactic acidosis was obtained by making the animals jump into the water and swim while carrying a load equivalent to 50% of body weight for 6 min (30-s exercise interrupted by a 30-s rest). After a 9-min rest, blood was collected and the incremental swimming test was started. The test consisted of swimming while supporting loads of 4.5, 5.0, 5.5, 6.0 and 7.0% of body weight. Each exercise load lasted 5 min and was followed by a 30-s rest during which blood samples were taken. The blood lactate minimum was determined from a zerogradient tangent to a spline function fitting the blood lactate vs workload curve. AT was estimated to be 4.95 ± 0.10% of body weight while interpolated blood lactate was 7.17 ± 0.16 mmol/l. These results suggest the application of AT determination in animal studies concerning metabolism during exercise.

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“…The double effort test underestimated the aerobic capacity of the animals in 13% (control) and 8.7% (obese); however, significant correlation was found between the tests for the animals from the control group (r = 0.88; P < 0.0075) and obese group (r = 0.92; P < 0.0031) (tables 1 and 2). Other studies, such as the ones by Pilis et al 29 and Langfort et al 30 , which evaluated the LAn using the progressive test of multistages, estimated the zone of metabolic transition through individual charts of the lactate concentrations versus velocity and found higher LAn intensities (25 m.mn -1 ). The differences found in the MLSS lactacidemic concentrations and the double effort test also suggest possible protocoldependence as observed in the study by Manchado-Gobatto et al 17 ; however, the double effort test presented high correlation with the MLSS both for the control and obese animals which presented alterations in insulin sensitivity, suggesting possible applicability in populations with any metabolic disorder.…”

Section: Discussionmentioning

confidence: 94%

Uso do teste de duplos esforços para avaliação da capacidade aeróbia de ratos obesos induzidos por dieta

Brandão¹,

Souza²,

Trombetta³

et al. 2013

Rev Bras Med Esporte

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Introduction: The literature lacks studies about lactate actions and some limitations in studies involving healthy individuals or patients with some metabolic disorder. Objectives: This study aimed to evaluate the protocol of double effort test for obese-induced rats. Methods: Fourteen male Wistar rats were divided into two groups: Control (Con) and Obese (Obe). The control group was fed with standard chow and water ad libitum. The obese group was fed with standard chow, water ad libitum and hyperlipidic diet. Twelve weeks after the beginning of the hyperlipidic diet, insulin tolerance test, Maximal Lactate Steady State (MLSS) test and the double efforts test were performed. Results: The diet was effective to promote obesity. The obese group decreased insulin sensitivity in approximately 19% (Con = 2.156 ± 0.1187 AU vs Obe = 1.742 ± 0.1551 AU). The lactate concentration and velocity of anaerobic threshold at MLSS test were 3.780 ± 0.09 mmol/L e 18 m.min -1 in both groups. The velocity of anaerobic threshold estimated by double efforts test was 15.59±0.653 m.min -1 in Con group control animals and 16.42±0.672 m.min -1 in Obe group. The double effort test underestimated around 13% and 8.7% the aerobic capacity in control and obese groups respectively, however, presented significant correlation with MLSS (r = 0,88; P < 0,0075 controls / r = 0,92; P < 0,0031 obese). Conclusion: So, the double effort test can be an interesting alternative to evaluate the aerobic capacity for both healthy sedentary and obese animals.

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Effect of sustained hyperadrenalinemia on exercise performance and lactate threshold in rats

Cited by 23 publications

References 23 publications

Increased lactate threshold after five weeks of treadmill aerobic training in rats

Increased lactate threshold after five weeks of treadmill aerobic training in rats

Determination of anaerobic threshold in rats using the lactate minimum test

Uso do teste de duplos esforços para avaliação da capacidade aeróbia de ratos obesos induzidos por dieta

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