Effect of Surface Modification on Viability of L929 Cells on Zirconia Nanocomposite Substrat

Abstract

IntroductionTitanium and its alloys as a dental implant have shown a high successful rate.1 Most metals or alloys are prone to corrosion process. Therefore, management and control of corrosion is a crucial point from biological aspects and may not limit to a local problem because the produced particles may migrate to far sites from the implant. Also it is important in terms of biocompatibility.2 So, the inert ceramic materials were suggested as alternatives to metals.3 Zirconia ceramic has been included in dentistry for different applications. In the design of implant, type of material and surface conditions have an impact on reliable osseointegration.5 Also, the degree of roughness is important. The different methods have been used to design the surface roughness.6 Beside effects of micro and nanoscale topography on different cells functions have examined in numerous studies. [7][8][9][10][11][12][13] This result has been explained by good cellular behavior. 7,10,13,14 In vitro and animal studies on zirconia have confirmed good biocompatibility and mechanical strength. 15,16 The osteoblastic response to different surface topographies of zirconia showed the higher cell proliferation in rough surfaces as compared to the smooth surface.17 Therefore the type of modification on morphology and viability L929 on A-Y-TZP20 substrate was evaluated by an invitro method. Methods Deposition of Hydroxyapatite Methods:In study evaluated viability of fibroblast cell following surface treatment. Therefore, viability L929 cells were characterized using MTT assay and scanning electron microscopy. Results:The viability assessment determined significant differences A-Y-TZP20 without surface treatment as compared to laser surface treatment (B), laser surface treatment + hydroxyapatiteyttrium stabilized tetragonal zirconia nanocomposite coat (C) and control. This study demonstrated that L929 cells approximately proliferated and spread on A-Y-TZP20 nanocomposite disk in laser surface treatment(B), Laser surface treatment + hydroxiapatite-yttrium stabilized tetragonal zirconia nanocomposite coat (C) groups similar to control group. Conclusion: Laser surface treatment showed positive effect on the viability of L929 cells.

An introduction of biological performance of zirconia with different surface characteristics: A review

“…It was suggested that laser irradiation produced adequate surface roughening of zirconia to support osseointegration 49) . It is noteworthy that another study showed that laser surface treatment showed positive effect on the viability of L929 cells 50) . Recently, micro-structured and nano-structured zirconia surfaces with higher surface roughness fabricated by solid-state laser sculpting were investigated.…”

“…Zirconia in dentistry has gained a vast amount interest in the last decade, having found its applications in prosthetic and implant dentistry. Laboratory studies have been focusing on modification of physico-chemical surface properties of zirconia for cementation 64,[102][103][104][105][106][107][108] zirconia porcelain bonding 109) and also to improve the biological performance of zirconia materials 39,41,50) . The results above have showed that many proposed surface treatment methods might improve the biological performance of zirconia used as a dental restoration or implant fixture.…”

Zirconia-based nanomaterials: recent developments in synthesis and applications

“…Aivazi et al 123 reported the laser method for the modication of the surface to support osseointegration. In this study, surface modication caused a positive impact on the viability of broblast L929 cells.…”

“…Methods such as sandblasting, acid etching, and laser etching have been performed for this purpose. 122 Aivazi et al 123 reported the laser method for the modification of the surface to support osseointegration. In this study, surface modification caused a positive impact on the viability of fibroblast L929 cells.…”

Surface Modifications for Zirconia Dental Implants: A Review

“…Fiber lasers, which can make 2 µm-wide grooves, were proved to produce adequate roughness on zirconia surfaces and increase new bone formation and mechanical strength on the bone-implant interface (36). Additionally, laser technologies positively affect wettability, acting as a critical determinant of cell adhesion, proliferation, and calcification, contributing to accelerating zirconia osseointegration (37,38). Studies showed that laser treatment would not exhibit phase transformation to zirconia while improving mechanical properties (39,40).…”