Effect of sugars on characteristics of Boer goat semen after cryopreservation

Naing, Soe; Wahid, H.; Azam, K. M.; Rosnina, Y.; Zuki, A.B.Z.; Kazhal, S.; Bukar, Mohammed; Thein, Myint; Kyaw, T.; San, Maung Maung

doi:10.1016/j.anireprosci.2010.06.006

Cited by 58 publications

(39 citation statements)

References 33 publications

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“…Boer buck spermatozoa post thawing with an intact acrosome in fructose egg yolk and glycerol egg yolk extender were 63.92±1.65 and 64.50±2.34, respectively (30).…”

Section: Discusionsmentioning

confidence: 94%

Preservation Effect of Crude Fig Fruit Filtrate (Ficus carica L) Added In to Tris Egg Yolk Based Extender on Capacitating, Acrosome and Fertility of Half Blood Boer Buck Spermatozoa

Zaenuri¹,

Susilawati²,

Wahyuningsih³

et al. 2014

IOSRJAVS

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“…Boer buck spermatozoa post thawing with an intact acrosome in fructose egg yolk and glycerol egg yolk extender were 63.92±1.65 and 64.50±2.34, respectively (30).…”

Section: Discusionsmentioning

confidence: 94%

Preservation Effect of Crude Fig Fruit Filtrate (Ficus carica L) Added In to Tris Egg Yolk Based Extender on Capacitating, Acrosome and Fertility of Half Blood Boer Buck Spermatozoa

Zaenuri¹,

Susilawati²,

Wahyuningsih³

et al. 2014

IOSRJAVS

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“…In our previous studies [10,17] lactose was used for sperm cryopreservation, while other monosaccharides such as glucose, fructose and sorbitol have never been examined. The effect of type of sugar supplementation in the freezing extenders on the post-thawed semen quality has been reported in the dog [29], buck [22], bull [28], ram [1] and stallion [24]. The stallion's cryopreserved sperms utilize sorbitol in the semen extender better than fructose and glucose [24].…”

mentioning

confidence: 99%

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

Chanapiwat

Kaeoket

Tummaruk

2012

The Journal of Veterinary Medical Science

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ABSTRACT. The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolkbased extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined at 0, 2 and 4 hr after thawing. It was found that the lactose-based extender resulted in a higher percentage of post-thawed sperm motility, viability, intact acrosome and functional plasma membrane than sorbitol-based extender (P<0.05) and fructose-based extender yielded a higher post-thawed sperm motility and viability than sorbitol-based extender (P<0.05). It could be concluded that sorbitol was not an effective sugar for the cryopreservation in boar semen. Boar semen cryopreservation greatly facilitates the distribution of valuable and desirable genes because the frozen semen can be transported over a long distance; and a stock of frozen semen enables the use of boar that is dead or ill [3]. However, the use of frozen-thawed (FT) boar semen is limited due to the low fertility outcomes compared to the extended fresh semen [9]. During cryopreservation, both physical and chemical factors including the rapid change in temperature or thermal stress, oxidative stress and osmotic stress lead to the damage of the sperm plasma membrane [19]. In general, the egg yolk-based freezing extender of boar semen consists of cryoprotectant, sugar, buffer and antibiotic [16]. Glycerol is the most common cryoprotectant used in the egg yolk-based semen extender for cryopreservation of the boar semen [14] and egg yolk is regarded as a non-permeable cryoprotectant. Sugar is mainly an energy source of the boar sperm. Furthermore, it protects the boar sperm from dehydration and intracellular ice formation during the semen freezing process [27]. Different types of sugar, e.g., trehalose, lactose, fructose, have been used in the freezing extender of boar semen [15,18].Recently, special interest on researches concerning with the energy utilization of the boar sperm have been noticed [13,15,18,20]. In general, the boar sperm is able to utilize a variety of substrates to obtain energy. Generally, monosaccharides are more common energy source used in extended fresh boar semen than disaccharides [20]. However, lactose (disaccharide) is commonly used for the cryopreservation of boar semen by many researchers [8,9,16,17]. In our previous studies [10,17] [24]. The stallion's cryopreserved sperms utilize sorbitol in the semen extender better than fructose and glucose [24]. In the boar, information associated with the effect of sugar types used in egg yolk-based semen extender on the FT semen qualities is limited. Therefore, the objective of the present study was to determine the effect of different sugar types (lactose, fructose, glucose and sorbitol) used in egg yolk-based semen extender on the sperm motility, sperm viability, acrosome integrity a...

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“…In humans, maximum sperm motility was observed at 1 mM [6], while the long-standing human sperm motility was improved at regular medium glucose concentration (5 mM) [12]. However, in Boer goat post-thaw semen, glucose supplementation as high as 70 mM increased sperm forward motility after cryopreservation [13]. The glucose concentration used to increase mouse sperm beat frequency was 5 mM during early hyperactivation [2].…”

Section: Introductionmentioning

confidence: 96%

Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility: An in vitro study

Chen

Pan

Lai

et al. 2015

Reproductive Biology

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Effect of sugars on characteristics of Boer goat semen after cryopreservation

Cited by 58 publications

References 33 publications

Preservation Effect of Crude Fig Fruit Filtrate (Ficus carica L) Added In to Tris Egg Yolk Based Extender on Capacitating, Acrosome and Fertility of Half Blood Boer Buck Spermatozoa

Preservation Effect of Crude Fig Fruit Filtrate (Ficus carica L) Added In to Tris Egg Yolk Based Extender on Capacitating, Acrosome and Fertility of Half Blood Boer Buck Spermatozoa

Cryopreservation of Boar Semen by Egg Yolk-Based Extenders Containing Lactose or Fructose is Better Than Sorbitol

Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility: An in vitro study

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