Effect of substrate stiffness on proliferation and differentiation of periodontal ligament stem cells

Liu, Nanxin; Zhou, Mi; Zhang, Qi; Li, Yong; Zhang, Tao; Tian, Taoran; Ma, Qing; Lin, Shiyu; Zhu, Bofeng; Cai, Xiaoxiao

doi:10.1111/cpr.12478

Cited by 34 publications

(24 citation statements)

References 48 publications

(90 reference statements)

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“…After cells were incubated with or without tFNAs for 6, 12, 24, and 36 h, the serum‐free medium was discarded, and then freshly prepared CCK‐8 solutions were added to each well. The absorbance of the CCK‐8 solution was measured to count the cells according to manufacturer's instruction of Cell Counting Kit‐8 (CCK‐8, MCE, USA) …”

Section: Methodsmentioning

confidence: 99%

Tetrahedral Framework Nucleic Acids Promote Corneal Epithelial Wound Healing in Vitro and in Vivo

Liu

Zhang

et al. 2019

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Poor post‐traumatic wound healing can affect the normal function of damaged tissues and organs. For example, poor healing of corneal epithelial injuries may lead to permanent visual impairment. It is of great importance to find a therapeutic way to promote wound closure. Tetrahedral framework nucleic acids (tFNAs) are new promising nanomaterials, which can affect the biological behavior of cells. In the experiment, corneal wound healing is used as an example to explore the effect of tFNAs on wound healing. Results show that the proliferation and migration of human corneal epithelial cells are enhanced by exposure to tFNAs in vitro, possibly relevant to the activation of P38 and ERK1/2 signaling pathway. An animal model of corneal alkali burn is established to further identify the facilitation effect of tFNAs on corneal wound healing in vivo. Clinical evaluations and histological analyses show that tFNAs can improve the corneal transparency and accelerate the re‐epithelialization of wounds. Both in vitro and in vivo experiments show that tFNAs can play a positive role in corneal epithelial wound healing.

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Section: Methodsmentioning

confidence: 99%

Tetrahedral Framework Nucleic Acids Promote Corneal Epithelial Wound Healing in Vitro and in Vivo

Liu

Zhang

et al. 2019

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“…Substrate stiffness, acting as a vital biomechanical factor, has been reported to modulate cellular functions in previous 7 Stem Cells International studies [18,19,38]. It has been demonstrated that MSCs favor neuronal differentiation on relatively soft substrates, whereas they undergo osteogenic differentiation as the substrate stiffness increases [14].…”

Section: Discussionmentioning

confidence: 99%

“…Besides, another study on bone marrowderived stem cells demonstrated that low-stiffness gels promoted cell proliferation, whereas high-stiffness gels facilitated cell osteogenic differentiation [11]. The stiffness induced behavioral differences among the different kinds of reproducible cells, including dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) within the dental area [18,19], and others such as valve interstitial cells (VICs) [20] and oral squamous cell carcinoma cells [21] have been investigated, thus bringing more significance to this parameter in tissue engineering and regenerative therapies. It has been established based on the various previous studies on mesenchymal stem cells (MSCs) that the softer substrate matrix is suitable to induce neurogenesis and adipogenesis, while stiffer matrix tended to induce myogenesis chondrogenesis and osteogenesis [14,[22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Stiffness Regulates the Morphology, Adhesion, Proliferation, and Osteogenic Differentiation of Maxillary Schneiderian Sinus Membrane-Derived Stem Cells

Liu

Wang

Zhai

et al. 2021

Stem Cells International

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Recent studies, which aim to optimize maxillary sinus augmentation, have paid significant attention exploring osteogenic potential of maxillary Schneiderian sinus membrane-derived cells (MSSM-derived cells). However, it remains unclear that how MSSM-derived cells could respond to niche’s biomechanical properties. Herein, this study investigated the possible effects of substrate stiffness on rMSSM-derived stem cell fate. Initially, rMSSM-derived stem cells with multiple differentiation potential were successfully obtained. We then fabricated polyacrylamide substrates with varied stiffness ranging from 13 to 68 kPa to modulate the mechanical environment of rMSSM-derived stem cells. A larger cell spreading area and increased proliferation of rMSSM-derived stem cells were found on the stiffer substrates. Similarly, cells became more adhesive as their stiffness increased. Furthermore, the higher stiffness facilitated osteogenic differentiation of rMSSM-derived stem cells. Overall, our results indicated that increase in stiffness could mediate behaviors of rMSSM-derived stem cells, which may serve as a guide in future research to design novel biomaterials for maxillary sinus augmentation.

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“…Indeed, YAP/TAZ also stimulates cell proliferation by controlling the expression of a broad number of cell cycle regulators, of factors involved in DNA duplication and DNA repair, and of factors involved in mitosis ( Zhao et al, 2007 ; Zanconato et al, 2015 , 2019 ). Moreover, a soft substrate is known to decrease cellular proliferation rate ( Mih et al, 2012 ; Liu et al, 2018 ). Although these cellular changes seemed to result mainly from the stiffness difference between the two models, we used a collagen and fibronectin coating to facilitate cell attachment on the leaf scaffold and we cannot exclude this did not also affect cell behavior.…”

Section: Discussionmentioning

confidence: 99%

Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models

Lacombe

Harris

Zenhausern

et al. 2020

Front. Bioeng. Biotechnol.

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Plant-based scaffolds present many advantages over a variety of biomaterials. Recent studies explored their potential to be repopulated with human cells and thus highlight a growing interest for their use in tissue engineering or for biomedical applications. However, it is still unclear if these in vitro plant-based scaffolds can modify cell phenotype or affect cellular response to external stimuli. Here, we report the characterization of the mechano-regulation of melanoma SK-MEL-28 and prostate PC3 cells seeded on decellularized spinach leaves scaffolds, compared to cells deposited on standard rigid cell culture substrate, as well as their response to drug and radiation treatment. The results showed that YAP/TAZ signaling was downregulated, cellular morphology altered and proliferation rate decreased when cells were cultured on leaf scaffold. Interestingly, cell culture on vegetal scaffold also affected cellular response to external stress. Thus, SK-MEL-28 cells phenotype is modified leading to a decrease in MITF activity and drug resistance, while PC3 cells showed altered gene expression and radiation response. These findings shed lights on the decellularization of vegetal materials to provide substrates that can be repopulated with human cells to better reproduce a soft tissue microenvironment. However, these complex scaffolds mediate changes in cell behavior and in order to exploit the capability of matching physical properties of the various plant scaffolds to diverse physiological functionalities of cells and human tissue constructs, additional studies are required to better characterize physical and biochemical cell-substrate interactions.

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Effect of substrate stiffness on proliferation and differentiation of periodontal ligament stem cells

Abstract: Results suggested that the osteogenic differentiation of PDLSCs might be promoted by culturing them in a stiffness-dependent manner, which regulates the Notch pathway. This might provide a new method of enhancing osteogenesis in PDLSCs.

Cited by 34 publications

References 48 publications

Tetrahedral Framework Nucleic Acids Promote Corneal Epithelial Wound Healing in Vitro and in Vivo

Tetrahedral Framework Nucleic Acids Promote Corneal Epithelial Wound Healing in Vitro and in Vivo

Stiffness Regulates the Morphology, Adhesion, Proliferation, and Osteogenic Differentiation of Maxillary Schneiderian Sinus Membrane-Derived Stem Cells

Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models

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