Effect of stevia on the gut microbiota and glucose tolerance in a murine model of diet-induced obesity

Becker, Sarah; Chiang, Edna; Plantinga, Anna; Carey, Hannah V.; Suen, Garret; Swoap, Steven J.

doi:10.1093/femsec/fiaa079

Cited by 23 publications

(23 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Conversely, in recent years, the effect of non-caloric sweeteners on the regulation of glucose levels has also been evaluated. The results to date are controversial, finding that some artificial sweeteners induce glucose intolerance (37). This may be due to the type of noncaloric sweetener consumed, its absorption and transport in the small and large intestines, as well as shifts in gut microbiota (38).…”

Section: Discussionmentioning

confidence: 99%

Maternal Sweeteners Intake Modulates Gut Microbiota and Exacerbates Learning and Memory Processes in Adult Male Offspring

et al. 2022

View full text Add to dashboard Cite

Background: There is increasing evidence that gut microbiota in offspring is derived in part from maternal environment such as diet. Thus, sweeteners intake including caloric or non-caloric during perinatal period can induce gut dysbiosis and program the offspring to develop cognitive problems later in life.Objective: To determine the effect of maternal high-sweeteners intake during gestation and lactation on gut microbiota shifts in adult male offspring rats and the impact on cognitive dysfunction.Methods: Thirty-four male pups from dams fed standard diet (Control-C, n = 10), high-sucrose diet (HS-C, n = 11), high-honey diet (Ho-C, n = 8), and high-stevia diet (HSt-C, n = 5) were fed standard diet after weaning, and body weight and food intake were recorded once a week for 26 weeks. Learning and memory tests were performed at week 23 of life using the Barnes maze. Fecal samples from the breastfeeding and adulthood periods were collected and analyzed by sequencing the 16S rRNA V3-V4 region of gut microbiota.Results: Maternal high-sucrose and stevia diets programmed the male offspring, and changes in microbial diversity by Shannon index were observed after weaning (p < 0.01). Furthermore, maternal high-stevia diet programming lasted into adulthood. The increase of Firmicutes abundance and the decrease in phylum Bacteroidetes were significant in HS-C and HSt-C groups. This led to an increase in the Firmicutes/Bacteroidetes index, although only in HS-C group was statistically significant (p < 0.05). Of note, the downstream gram-negative Bacteroidales and the upregulation of the gram-positive Clostridiales abundance contribute to cognitive dysfunction.Conclusion: These results suggest that dams fed a high-sucrose and stevia diets during gestation and lactation favor a deficient memory performance in adult male offspring rats through shifts gut microbiota diversity and relative abundance at several taxa.

show abstract

Section: Discussionmentioning

confidence: 99%

Maternal Sweeteners Intake Modulates Gut Microbiota and Exacerbates Learning and Memory Processes in Adult Male Offspring

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Diet can rapidly alter the gut microbiome community in as short as 24 h (David et al, 2014). For example, many laboratory studies of adult rodents have shown that a typical Western diet (high in fat and sugar) alters the gut microbiome community and reduces diversity of bacterial species (Becker et al, 2020;Beilharz et al, 2017;Leamy et al, 2014;Pindjakova et al, 2017;Turnbaugh et al, 2008). In multiple strains of inbred, outbred and transgenic mice, a shift in diet can have lasting effects on the community, as repetitive switching from a high-fat, high-sugar diet to a low-fat diet results in altered community membership and composition that does not revert to the original state (Carmody et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Early-life effects of juvenile Western diet and exercise on adult gut microbiome composition in mice

McNamara

Singleton

Cadney

et al. 2021

Journal of Experimental Biology

View full text Add to dashboard Cite

Alterations to the gut microbiome caused by changes in diet, consumption of antibiotics, etc., can affect host function. Moreover, perturbation of the microbiome during critical developmental periods potentially have long-lasting impacts on hosts. Using four selectively bred High Runner and four non-selected Control lines of mice, we examined the effects of early-life diet and exercise manipulations on the adult microbiome by sequencing the hypervariable Internal Transcribed Spacer region of the bacterial gut community. Mice from High Runner lines run ∼3-fold more on wheels than do Controls, and have several other phenotypic differences (e.g., higher food consumption and body temperature) that could alter the microbiome, either acutely or in terms of coevolution. Males from generation 76 were given wheels and/or Western diet from weaning until sexual maturity at 6 weeks of age, then housed individually without wheels on standard diet until 14 weeks of age, when fecal samples were taken. Juvenile Western diet reduced bacterial richness and diversity after the 8-week washout period (equivalent to ∼6 human years). We also found interactive effects of genetic linetype, juvenile diet, and/or juvenile exercise on microbiome composition and diversity. Microbial community structure clustered significantly in relation to both linetype and diet. Western diet also reduced the relative abundance of Muribaculum intestinale. These results constitute one of the first reports of juvenile diet having long-lasting effects on the adult microbiome after a substantial washout period. Moreover, we found interactive effects of diet with early-life exercise exposure, and a dependence of these effects on genetic background.

show abstract

“…Also, Rosales-Gómez et al (2018) showed that stevia has increased glycaemia and the HOMA index, evidencing insulin resistance, in healthy mice. Also, Becker et al (2020) showed that stevia changed the gut microbiota as did saccharin when consumed with a high fat diet.…”

Section: Discussionmentioning

confidence: 99%

The hidden hazardous effects of stevia and sucralose consumption in male and female albino mice in comparison to sucrose

Farid

Hesham

El-Dewak

et al. 2020

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

Replacing sucrose with non-caloric sweeteners is an approach to avoid overweight and diabetes development. Non-caloric sweeteners are classified into either artificial as sucralose or natural as stevia. Both of them have been approved by FDA, but the effects of their chronic consumption are controversial. The present study aimed to evaluate the effects of these two sweeteners, in male and female albino mice, on different blood biochemical parameters, enzymes activities and immunological parameters after 8 and 16 weeks of sweeteners administration. 40.5 mg/ml of sucrose, 5.2 mg/ml of sucralose and 4.2 mg/ml of stevia were dissolved individually in distilled water. Mice were administrated by sweetener's solution for 5 h daily. Male and female mice showed a preference for water consumption with sucralose or stevia. Both of the two sweeteners significantly reduced the hemoglobin level, HCT%, RBCs and WBCs count. After 18 weeks, significant elevations in liver and kidney function enzymes were observed in male and female mice administrated with both non-caloric sweeteners. Histopathological examination in sucralose and stevia administrated groups confirmed the biochemical results; where it revealed a severe damage in liver and kidney sections. While, sucrose administration elevated, only, the levels of ALT, AST and cholesterol in male mice. A vigorous elevation in levels of different immunoglobulin (IgG, IgE and IgA) and pro-inflammatory cytokines (IL-6 and -8), that was accompanied by a significant reduction in level of anti-inflammatory cytokine IL-10, was observed in male and female mice groups administrated with sucralose or stevia. On the other hand, sucrose administration led to an elevation in IgA and reduction in IL-10 levels.

show abstract

Effect of stevia on the gut microbiota and glucose tolerance in a murine model of diet-induced obesity

Cited by 23 publications

References 72 publications

Maternal Sweeteners Intake Modulates Gut Microbiota and Exacerbates Learning and Memory Processes in Adult Male Offspring

Maternal Sweeteners Intake Modulates Gut Microbiota and Exacerbates Learning and Memory Processes in Adult Male Offspring

Early-life effects of juvenile Western diet and exercise on adult gut microbiome composition in mice

The hidden hazardous effects of stevia and sucralose consumption in male and female albino mice in comparison to sucrose

Contact Info

Product

Resources

About