Co x Ni 0.5 Cd 0.5-x Fe 2 O 4 (x = 0.0, 0.1, 0.2, 0.3, 0.4, 0.5) nanoferrites were synthesized by citrate-gel auto combustion method. Antimicrobial study was tested on Gram-negative bacteria and Gram-positive bacteria by adopting disc-diffusion agar method. The prepared nanoferrites show excellent antimicrobial function with a zone of inhibition of 10-12 mm against E. coli, 5-7 mm against K. pneumonia, 9-12 mm against B. subtilis and 5-7 mm against S. aureus. Inhibition of the growth was ascertained with reference to Ampicillin, a standard antibacterial drug, that acts as positive control. The antibacterial properties of the prepared ferrites bid great challenges in biomedical and pharmaceutical applications.