Effect of Silymarin Extracted From Silybum Marianum on Nickel Hematotoxicity and Nephrotoxicity in Male Albino Wistar Rats

Abstract

Objective: The objective of this study was to investigate the effect of silymarin extract from Silybum marianum against nickel-induced alterations in haematological indices, kidney dysfunction and renal antioxidant defence system. Methods:Male albino Wistar rats were divided into four groups seven each. Control, silymarin, nickel and nickel plus silymarin. Silymarin was administrated orally (100 mg/kg b. wt) and nickel as nickel sulfate (NiSO4 6H2 Results:The treatment with nickel led to a significant decrease in body weight with an increase in both absolute and relative kidney weights and a significant increase in renal markers, which confirmed by histopathological alteration. A microcytic anemia was also observed, which was manifested by a reduction of red blood cells count (RBC), hemoglobin (Hb) concentration, platelet counts (Plt), hematocrit and white blood cells counts (WBC). The level of lipid peroxidation was increased. Whereas, GSH concentration and enzymatic antioxidants SOD, GSH-Px and CAT activities were decreased. The co-treatment with methanolic extract of milk thistle attenuated the variation in the hematological and renal markers, decreasing renal lipid peroxidation (p<0.05) with a concomitant increasing reduced glutathione content (p<0.01) and restoring the antioxidant enzymes (SOD, CAT, GSH-Px) in kidney, as well as an improvement in histological changes compared to those previously noticed in nickel group. 0) was given intraperitoneally (20 mg/kg b. wt) at alternative days. The experiment continued for three consecutive weeks. Body weight was recorded regularly. After overnight fasting, animals were killed and serum creatinine, serum urea, serum uric acid, hematological parameters and renal antioxidant markers were determined. Conclusion:To conclude, these findings demonstrated that silymarin extract effectively improved heamatotoxicity and nephrotoxicity caused by nickel.

Ameliorative Role of Silymarin Against Aspartame–induced Biochemical Changes, Oxidative Stress, Inflammatory Effect and Genotoxicity in Male Albino Rats

“…In group 5, silymarin administration decreased the elevation in glucose level which was statistically non-significant (P˃ 0.05) compared to the control group. These results were in agreement with who reported that there was an amelioration of blood glucose concentration in nickel animals treated with silymarin extract [50]. It was suggested that the protective role of silymarin was due to its antioxidant properties, an expansion of plasma and pancreatic glutathione concentrations or both [51].…”

The Benefit Effect of Ginger Supplementation Against Nickel-Induced Hepatotoxicity in Albino Wistar Rats

“…Ni is a well-known cytotoxic metal that may affect various organs, particularly liver cells [8]. The metal generates reactive oxygen species (ROS), which lead to lipid peroxidation (LPO), oxidation of DNA, and proteins and results in cell apoptosis [9], immunotoxic [10], hematotoxicity [11], neurotoxic [12], reproductive toxic [13], pulmonary toxic [14], hepatotoxicity [15], and nephrotoxicity [16]. A number of medicinal plants have been described to acquire ROS scavenging and cytoprotective properties [17,18].…”

Relaxation Activity of Tetanus (Leea Aequata L.) Leaf Ethanolic Extract on Guinea Pig Isolated Trachea

“…Thus, traditional medicine is a field that is still in a great demand for research [2]. The traditional medicine aslo safe and no toxic compared to conventional medicine [3,4].…”