Effect of Short-term Tumour Necrosis Factor-alpha (TNF-α) -stimulation on the Growth and Differentiation of MC3T3-E1 Osteoblast-like Cells

Abstract

Tumor necrosis factor-alpha (TNF-α) is an infl ammatory cytokine known to cause bone resorption, swelling and edema during tissue organization. Conversely, TNF-α has also been shown to participate in tissue regeneration during the wound healing process. We have previously investigated the eff ects of TNF-α on human dental pulp cell diff erentiation. Dental pulp cells are composed of diff erent cell types including primary odontoblasts and fi broblasts. We determined that the ratio of stem cells within the pulp cell population was increased following short-term stimulation with TNF-α. The aim of this study therefore was to investigate the eff ect of short-term stimulation with TNF-α on osteoblast-like MC3T3-E1 cell growth and diff erentiation. MC3T3-E1 cells were cultured in standard growth medium and on reaching sub-confl uence were exposed to recombinant TNF-α (10 and 100 ng/ml) for 2 days prior to assessing their cell proliferation and diff erentiation properties in comparison to non-stimulated MC3T3-E1 cells (control). Although no signifi cant diff erences in cell proliferation were observed between the TNF-α-stimulated and control groups, cell diff erentiation was delayed in the TNF-α-stimulated groups. In summary, short-term stimulation of cultured MC3T3-E1 cells with TNF-α had only minimal eff ect on their growth and diff erentiation.

Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)

“…Several studies have reported that TNF-α inhibited Type I collagen production and ALP expression based on the blockage of osteoblast differentiation in osteoblastic clonal cell lines [33][34][35] . Furthermore, Inoue et al have shown that short-term stimulation by TNF-α delayed cell differentiation in osteoblast-like cells 36) . In this study of a heterogeneous BMSC population, it is not clear if there are different bone marrow subpopulations that acquire stem cell properties after TNF-α stimulation 37) .…”

Effects of αCGRP on the Adhesion, Proliferation and Differentiation of Osteoblasts Cultured on Titanium Surfaces

“…Rigid osseointegration at the implant-bone interface is the functional foundation of dental implants. Bone around dental implants is continuously remodeled, and various factors have been demonstrated to regulate this process, including local cytokines, growth factors and systemic hormones 1,2) .…”