Effect of serum proteins and osteoblasts on the surface transformation of a calcium phosphate coating: A physicochemical and ultrastructural study

Radin, S.; Ducheyne, Paul; Berthold, Peter; Decker, Sylvia

doi:10.1002/(sici)1097-4636(199802)39:2<234::aid-jbm10>3.0.co;2-d

Cited by 48 publications

(8 citation statements)

References 18 publications

(22 reference statements)

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“…The mode of formation or at least the growth of the three‐dimensional crystalline latticework is probably influenced by the chemical nature of the bioagent and by the electrical charge of its surface, whereby different bonding strengths may be achieved. These parameters might have a bearing on the mode of seeding and thus on the number of nucleation sites that are generated . Protein molecules might also form themselves into aggregates, which could be penetrated by the inorganic ions during the formation of the coating.…”

Section: Discussionmentioning

confidence: 99%

The kinetics and mechanism of bone morphogenetic protein 2 release from calcium phosphate‐based implant‐coatings

Liu

Schouten

Boerman

et al. 2018

J Biomedical Materials Res

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Biomimetically deposited calcium phosphate-based coatings of prostheses can serve as a vehicle for the targeted delivery of growth factors to the local implant environment. Based on indirect evidence in previous studies we hypothesize that such agents are liberated gradually from the coating via a cell-mediated degradation. In the present study, we tested this hypothesis by investigating the release mechanism and its kinetics by use of a radiolabeled osteogenic agent ( I-BMP-2) under conditions in which native cell populations with a coating-degradative potential were either absent or present. The release of I-BMP-2 was monitored for 5 weeks, either in vitro or after implantation at an ectopic (subcutaneous) site in rats in vivo. Only from implants that bore a coating-incorporated depot of bone morphogenetic protein 2 (BMP-2) was the agent released slowly and steadily over 5 weeks, that is, 50% of the loaded dose was liberated in vivo (5 to 10% weekly), as against 14.6% in vitro (less than 1% weekly). The coatings bearing an incorporated depot of BMP-2 underwent significant cell-mediated degradation, whereas under cell-free conditions no degradation occurred, and the spontaneous release of BMP-2 was negligible. Our findings confirm this carrier system to be a suitable vehicle for the sustained and cell-mediated delivery of BMP-2. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2363-2371, 2018.

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Section: Discussionmentioning

confidence: 99%

The kinetics and mechanism of bone morphogenetic protein 2 release from calcium phosphate‐based implant‐coatings

Liu

Schouten

Boerman

et al. 2018

J Biomedical Materials Res

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“…Adhesion, proliferation, and differentiation of osteoblastic cells were reported to be either increased or decreased when cultured on HAP 23–26. Proliferation rate of periodontal ligament cells cultured on hydroxyapatite was reduced related to that on polystyrene; collagen synthesis, alkaline phosphatase activity, and mineralization were also found to be reduced 27.…”

Section: Discussionmentioning

confidence: 99%

Proliferation and differentiation of osteoblasts on Biocement D modified with collagen type I and citric acid

et al. 2004

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In this study, the proliferation and differentiation of rat calvarial osteoblasts cultured on either (1) calcium-phosphate bone cement Biocement D, (2) Biocement D with 2.5% (w/w) mineralized collagen type I, or (3) Biocement D with 2.5% (w/w) mineralized collagen type I and 3% (w/w) citric acid were investigated. Incubation of the composites in cell-culture medium resulted in a fast decrease of pH and calcium concentration as well as in an increase of phosphate concentration. Although these effects occurred with all investigated materials, the lowest extent could be observed for the citric-acid-containing composites. As shown by scanning-electron microscopy, osteoblasts adhered to the composite surfaces. Proliferation and differentiation of the cells grown on the composites were found to be reduced compared to cells grown on tissue-culture polystyrene. Cells cultured in the vicinity of the composites but without direct contact also exhibited a reduced rate of proliferation, reduced alkaline phosphatase activity, and reduced mineralization. Simulating the changes in calcium and phosphate concentration occasioned by the composites through exposing cells to EGTA and phosphate gives rise to the same effects of reducing proliferation, ALP activity, and mineralization. No indication for apoptosis in cells exposed to low calcium and high phosphate concentrations was found. The number of necrotic cells, however, increased after incubation with EGTA and phosphate. For assessment of cell-composite interactions and the success of the composites in vivo, as well as for more effective material development, it seems to be important to know how changes in microenvironmental pH and ion composition of the material affect cellular proliferation and differentiation.

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“…Degradation of the implant material depends on the physicochemical properties like chemical and phase composition, porosity, and matrix ultrastructure 6, 7. Moreover, graft‐resorption in vivo and in vitro is both a cell‐ and solution‐mediated phenomenon 8, 9. Cellular activity affects the dissolution reactions at the tissue‐implant interface 8.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, graft‐resorption in vivo and in vitro is both a cell‐ and solution‐mediated phenomenon 8, 9. Cellular activity affects the dissolution reactions at the tissue‐implant interface 8. However, the implant degradation rate can in turn affect the bone‐cell function.…”

Section: Introductionmentioning

confidence: 99%

Effects of exogenous phosphorus and silicon on osteoblast differentiation at the interface with bioactive ceramics

Gupta

Kirakodu

El‐Ghannam

2010

J Biomedical Materials Res

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In this study, we have investigated the effects of dissolved phosphorus and silicon on osteoblast differentiation in vitro. Neonatal rat calvarial osteoblasts were seeded on silica-calcium phosphate composites (SCPCS), hydroxyapatite (HA-200), and tissue culture polystyrene (TCPS) and incubated over 4 days in media containing 0 {minimal essential medium [MEM] (-)} or 3 mM β-glycerophosphate [MEM (+)]. Inductively coupled plasma analysis showed that P-content in original MEM (+) was 225% higher than that in MEM (-). Moreover, P-content in MEM (+) significantly increased to 3.4-4.4 mM and 3.6-4.7 mM after 2 and 4 days incubation with SCPC, respectively, owing to material dissolution and exogenous phosphate supplementation. In contrast, P-content in MEM (+) showed no change upon incubation with HA or TCPS. The P-content in MEM (-) incubated with SCPC was considerably lower than that in MEM (+). SCPC exhibited controlled Si-release in cell culture media [MEM (-) or MEM (+)], with Si-rich SCPC showing a significantly greater dissolution than Si-poor SCPC. Moreover, SCPC, unlike HA, demonstrated a cell- and solution-mediated dissolution over 4 days. Quantitative real-time PCR showed that in MEM (-), osteocalcin and osteopontin mRNA expression on Si-rich SCPC was significantly greater than that on HA, suggesting that Si plays an important role in enhancing bone-cell differentiation. However, osteoblast phenotypic expression on SCPC was significantly decreased after 4 days incubation in MEM (+), indicating that sustained exposure to elevated P-levels in the media can downregulate osteoblast function. Our results demonstrate that the controlled dissolution of SCPC provides a natural stimulus for bone-cell differentiation in vitro and could obviate the need of exogenous phosphate supplementation.

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Effect of serum proteins and osteoblasts on the surface transformation of a calcium phosphate coating: A physicochemical and ultrastructural study

Cited by 48 publications

References 18 publications

The kinetics and mechanism of bone morphogenetic protein 2 release from calcium phosphate‐based implant‐coatings

The kinetics and mechanism of bone morphogenetic protein 2 release from calcium phosphate‐based implant‐coatings

Proliferation and differentiation of osteoblasts on Biocement D modified with collagen type I and citric acid

Effects of exogenous phosphorus and silicon on osteoblast differentiation at the interface with bioactive ceramics

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