Effect of Seminal Plasma Components on the Quality of Fresh and Cryopreserved Stallion Semen

Suarez, Alexandra Usuga; Rojano, Benjamín; Betancur, Giovanni Restrepo

doi:10.1016/j.jevs.2017.09.005

Cited by 13 publications

(13 citation statements)

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“…All procedures were carried out in accordance with the UK Animals (Scientific Procedures) Act, 1986, and associated guidelines, EU Directive 2010/63/EU for animal experiments, or the National Institutes of Health guide for the Care and Use of Laboratory Animals (NIH Publications No. 8023, revised 1978; Usuga, Rojano, & Restrepo, ).…”

Section: Methodsmentioning

confidence: 99%

Relationship of cysteine‐rich secretory protein‐3 gene and protein with semen quality in stallions

Betancur

Rojano

Suarez

2018

Reprod Domestic Animals

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Cysteine-rich secretory protein-3 (CRISP-3) and some of its nonsynonymous polymorphism have been related to the fertility and freezability of stallion semen; however, the role of the CRISP-3 gene and its seminal plasma protein in the raw semen quality is still unknown. The aim of this study was to evaluate the relationship of CRISP-3 with semen quality in stallions. DNA was obtained from blood samples of 100 stallions, from which 30 stallions were randomly selected to obtain 60 ejaculates. Through PCR amplification and sequencing, the variation of four nonsynonymous SNPs from CRISP-3 was identified and haplotypes were derived. Semen quality was assessed through the total motility (MOT), sperm vitality (SV), normal morphology (NM), functional integrity of membrane (MI) and a seminal quality index (SQi). CRISP-3 protein content of seminal plasma (SP) was determined by ELISA. The effect of the genotype, the haplotype and the concentration of the CRISP-3 protein on the seminal quality were evaluated through generalized linear models and linear regression analyses. Homozygous genotypes for SNP1, SNP2 and SNP3 and the heterozygous genotype for SNP4 showed a positive effect on seminal quality. Different haplotypes with positive effect on MOT, SV, NM, MI and SQi were identified. The allelic substitution analysis resulted in positive regression coefficients for MOT (SNP2) and MI (SNP2 and SNP3). A high level of CRISP-3 resulted in a higher MOT and SQi. It is concluded that the quality of stallion semen is influenced by the genotype of CRISP-3 and the concentration of CRISP-3 protein in SP.

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Section: Methodsmentioning

confidence: 99%

Relationship of cysteine‐rich secretory protein‐3 gene and protein with semen quality in stallions

Betancur

Rojano

Suarez

2018

Reprod Domestic Animals

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“…Sin embargo, la adición de PS no generó efecto alguno sobre la morfología, la vitalidad o la integridad funcional de la membrana plasmática (Cuadro 3). Esto podría explicarse por la variabilidad en la composición del PS de los reproductores, toda vez que, en estudios previos se observó que diferentes niveles de proteínas, vitaminas y iones presentes en el PS influyen, no solo en la calidad del semen fresco, sino también en la calidad del semen criopreservado cuando se adiciona PS (Usuga et al, 2017(Usuga et al, , 2018. En un estudio reciente se encontró que la selección espermática mediante centrifugación de una sola capa (SLC) antes de la criopreservación mejoró la calidad espermática posdescongelación, y que la inclusión de PS de caballos «buenos» y «malos» congeladores no tuvo un efecto benéfico adicional (Al-Essawe et al, 2018a).…”

Section: Resultados Y Discusiónunclassified

Aporte antioxidante del plasma seminal y su efecto sobre la calidad del semen equino congelado

Betancur

Pizarro

Rojano

2019

Rev. investig. vet. Perú

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El plasma seminal (PS) posee antioxidantes enzimáticos y no enzimáticos con la función de proteger los espermatozoides de los efectos nocivos del estrés oxidativo. El objetivo de este estudio fue evaluar el aporte antioxidante del PS durante la congelación del semen equino y su efecto sobre la calidad espermática posdescongelación. Se colectó el semen de cinco caballos, se separó el PS y se suplementó en proporciones del 0% (Control), 10% (PS10) y 20% (PS20) a la fracción espermática en el diluyente de congelación. Se evaluó la capacidad antioxidante total (CAT) por los métodos de la capacidad atrapadora de radical oxígeno (ORAC) y de la capacidad reductora férrica (FRAP), y la actividad de las enzimas SOD, GPx y catalasa mediante espectrofotometría. Quince eyaculados fueron sometidos a congelación y se evaluó la movilidad (MOV), vitalidad (VE), morfología (MA), integridad funcional de membrana (IM) y el potencial de membrana mitocondrial (PMM) de los espermatozoides. Se ajustaron modelos mixtos, se compararon las medias por la prueba de Tukey y se realizó un análisis de correlación. PS10 y PS20 generaron un incremento de la CAT medida por FRAP y de la actividad de catalasa del semen diluido (p<0.05). PS20 produjo un aumento de la MOV del semen descongelado en comparación con el Control (p<0.05). Se observó una reducción posdescongelación del PMM por efecto de PS10 y PS20 (p<0.05), y se hallaron correlaciones positivas entre el PMM y la actividad de SOD. Se concluye que el PS aumenta la capacidad antioxidante total y enzimática del semen equino e influye en la actividad mitocondrial y la movilidad espermática posdescongelación.

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“…Other components of the SP, apart from those investigated in the present study, might also influence fertility parameters and freezability. Usuga et al, [10], focussing on other SP components and total protein content, found that a high level of vitamin A in the SP was associated with the best sperm quality, while low levels of vitamin E were associated with poor motility. In contrast, Mráčková et al [9] encountered lower vitamin E levels in ejaculates from good freezers.…”

Section: Discussionmentioning

confidence: 99%

“…The influence of the different components of SP on freezability has been reported by several authors. Vitamin E levels have been found to be lower in ejaculates from good freezers [9], a finding contradicted by Usuga et al, [10]. Usuga et al, [11] found the highest motility in frozen-thawed samples with high levels of cysteine-rich secretory protein-3 (CRISP-3) compared to samples with low or medium levels of CRISP-3.…”

Section: Introductionmentioning

confidence: 93%

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

Johannisson

Al-Essawe

Al-Saffar

et al. 2020

Journal of Reproduction and Development

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The mechanism by which the content of the major groups of seminal plasma proteins in stallion semen changes between the breeding and non-breeding seasons remains unknown. Here, we investigated the proportions of non-heparin-binding, phosphorylcholine-binding, and heparin-binding proteins in seminal plasma with the aim of relating them to sperm quality and testosterone levels in good and bad freezer stallions. Only minor variations in the major protein groups were found between the breeding and non-breeding seasons. In the non-breeding season, a higher content of a subset of non-heparin binding proteins as well as of heparin-binding proteins was found. Analysis of semen characteristics revealed a somewhat contrasting picture. While only minor variations in sperm kinematics and sperm morphology were found between seasons, the flow-cytometric measurements of mitochondrial membrane potential and also, to some extent, reactive oxygen species production indicated lower sperm quality in the breeding season. Chromatin integrity and testosterone levels were unchanged between seasons. The results suggest that stallion ejaculates could be used year-round for freezing, since only minor differences in protein composition exist between the breeding and non-breeding seasons, as well as between good and bad freezers. In addition, sperm quality is not impaired during the non-breeding season.

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Effect of Seminal Plasma Components on the Quality of Fresh and Cryopreserved Stallion Semen

Cited by 13 publications

References 46 publications

Relationship of cysteine‐rich secretory protein‐3 gene and protein with semen quality in stallions

Relationship of cysteine‐rich secretory protein‐3 gene and protein with semen quality in stallions

Aporte antioxidante del plasma seminal y su efecto sobre la calidad del semen equino congelado

Season does not have a deleterious effect on proportions of stallion seminal plasma proteins

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