Effect of Rhamnolipids and Lipopolysaccharides on the Bioleaching of Arsenic-Bearing Waste

Abstract

The adsorption of biosurfactants and polysaccharides changes the surface properties of solid particles, which is important for controlling the release of arsenic compounds from the solid phase and preventing undesirable bioleaching. Microbial leaching and scorodite adhesion experiments, including pure and modified mineral material, were conducted in a glass column with a mineral bed (0.8–1.2 mm particle size) to test how rhamnolipids (Rh) and lipopolysaccharides (LPS) affect surface properties of mineral waste from Złoty Stok (Poland) and secondary bio-extraction products (scorodite). Adsorption tests were conducted for both solid materials. The adsorption of Rh and LPS on the solids was shown to modify its surface charge, affecting bioleaching. The highest bio-extraction efficiency was achieved for arsenic waste with adsorbed rhamnolipids, while the lowest, for the LPS-modified mineral. Under acidic circumstances (pH~2.5), the strongly negative zeta potential of arsenic-bearing waste in the presence of Rh creates conditions for bacteria adhesion, leading to the intensification of metal extraction. The presence of a biopolymer on the As waste surface decreases leaching efficiency and favours the scorodite’s adhesion.

Current Trends in Metal Biomining with a Focus on Genomics Aspects and Attention to Arsenopyrite Leaching—A Review

“…Thus, both toxicity of the mineral ore material and resistance of the leaching bacteria have a great significance for biomining. There are various points of view on the problem of the arsenopyrite leaching which include not only toxicity, but also the formation of scorodite and solubility of the minerals surface [ 67 , 68 ].…”