Effect of recombinant human bone morphogenetic protein‐7 (rhBMP‐7) on the viability, proliferation and differentiation of osteoblast‐like cells cultured on a chemically modified titanium surface

Togashi, Adriane Yaeko; Cirano, Fabiano Ribeiro; Marques, Márcia Martins; Pustiglioni, Francisco Emílio; Lang, Niklaus P.; Lima, Luiz Antônio Pugliesi Alves de

doi:10.1111/j.1600-0501.2008.01669.x

Cited by 8 publications

(8 citation statements)

References 32 publications

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“…We and others have demonstrated that alkaline phosphatase activity, an early indicator of osteoblastic differentiation, and secreted osteocalcin, a later marker of osteoblastic differentiation, are higher in cells cultured on rough surfaces than on smooth substrates [25,26]. Our results showed that MSCs were affected by surface roughness independently of whether they were grown on Ti or graphitic carbon-coated surfaces, suggesting that surface roughness is a dominant factor in inducing osteoblastic differentiation of MSCs.…”

Section: Discussionsupporting

confidence: 54%

Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

et al. 2015

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Surface roughness, topography, chemistry, and energy promote osteoblast differentiation and increase osteogenic local factor production in vitro and bone-to-implant contact in vivo, but the mechanisms involved are not well understood. Knockdown of integrin heterodimer alpha2beta1 (α2β1) blocks the osteogenic effects of the surface, suggesting signaling by this integrin homodimer is required. The purpose of the present study was to separate effects of surface chemistry and surface structure on integrin expression by coating smooth or rough titanium (Ti) substrates with graphitic carbon, retaining surface morphology but altering surface chemistry. Ti surfaces (smooth [Ra<0.4μm], rough [Ra≥3.4μm]) were sputter-coated using a magnetron sputtering system with an ultrapure graphite target, producing a graphitic carbon thin film. Human mesenchymal stem cells and MG63 osteoblast-like cells had higher mRNA for integrin subunits α1, α2, αv, and β1 on rough surfaces in comparison to smooth, and integrin αv on graphitic-carbon-coated rough surfaces in comparison to Ti. Osteogenic differentiation was greater on rough surfaces in comparison to smooth, regardless of chemistry. Silencing integrins β1, α1, or α2 decreased osteoblast maturation on rough surfaces independent of surface chemistry. Silencing integrin αv decreased maturation only on graphitic carbon-coated surfaces, not on Ti. These results suggest a major role of the integrin β1 subunit in roughness recognition, and that integrin alpha subunits play a major role in surface chemistry recognition.

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Section: Discussionsupporting

confidence: 54%

Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

et al. 2015

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“…As mentioned above, the lower proliferation in the rough (coated and uncoated SLA) surfaces has been associated with an enhanced differentiation into the osteoblastic lineage. 28,29 This was evident from the results of the alkaline phosphatase activity and osteocalcin levels, early and late markers of osteoblastic differentiation, respectively. Both protein markers were significantly increased in the SLA-surfaces, but it was more notorious on the aSLA surfaces.…”

Section: Discussionmentioning

confidence: 93%

Enhancing the osteoblastic differentiation through nanoscale surface modifications

Silva-Bermúdez

Almaguer‐Flores

García

et al. 2016

J Biomedical Materials Res

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Human mesenchymal stem cells (MSCs) showed larger differentiation into osteoblasts on nanoscale amorphous titanium oxide (TiO ) coatings in comparison to polycrystalline TiO coatings or native oxide layers. In this article, we showed that the subtle alterations in the surface properties due to a different atomic ordering of titanium oxide layers could substantially modify the osteoblastic differentiation of MSCs. Amorphous (a) and polycrystalline (c) TiO coatings were deposited on smooth (PT) and microstructured sandblasted/acid-etched (SLA) Ti substrates using a magnetron sputtering system. The surface roughness, water contact angle, structure, and composition were measured using confocal microscopy, drop sessile drop, X-ray diffraction, and X-ray photoelectron spectroscopy, respectively. The ∼70-nm-thick coatings presented a well-passivated and uniform TiO (Ti ) surface composition, while the substrates (native oxide layer) showed the presence of Ti atoms in lower valence states. The polycrystalline TiO -coated surfaces (cPT and cSLA) showed the same cell attachment as the uncoated metallic surfaces (PT and SLA), and in both cases, it was lower on the rough than on the smooth surfaces. However, attachment and differentiation were significantly increased on the amorphous TiO -coated surfaces (aPT and aSLA). The amorphous coated Ti surfaces presented the highest expression of integrins and production of osteogenic proteins in comparison to the uncoated and crystalline-coated Ti surfaces. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 498-509, 2017.

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“… 6 , 26 When titanium was used in combination with BMP-7 in the medium to study osteogenic effects in vitro , results were not uniform. For example, one study showed no benefit from the presence of soluble BMP-7, 27 while other works suggested increased osteogenic potential in samples with BMP-7; 28 however, continuous supply of the growth factor was necessary. To improve osteogenesis, while using low amounts of BMP-7, we coated Ti samples with PEA that induces FN arrangement into fibrillar nanonetworks to allow specific binding of BMP-7 to FN.…”

Section: Discussionmentioning

confidence: 99%

“…Titanium implants have shown long-term clinical success in single and multiple restorations; however, they face the disadvantage of prolonged osseointegration time. , When titanium was used in combination with BMP-7 in the medium to study osteogenic effects in vitro , results were not uniform. For example, one study showed no benefit from the presence of soluble BMP-7, while other works suggested increased osteogenic potential in samples with BMP-7; however, continuous supply of the growth factor was necessary. To improve osteogenesis, while using low amounts of BMP-7, we coated Ti samples with PEA that induces FN arrangement into fibrillar nanonetworks to allow specific binding of BMP-7 to FN. ,, Even though FN has been previously investigated as coating material for dental implants, no significant effects has been observed in vitro or in vivo . − Corresponding to the important role of FN in ECM formation, FN nanonetworks on PEA have shown to promote cell adhesion and differentiation with different cell types, ,, and more importantly, enhanced signaling via synergistic effect between FNIII9–10 (integrin binding region) and FNIII12–14 (GF binding domain) has been described using BMP-2 and VEGF bound to FN on PEA. , In our Ti coatings, bound BMP-7 on assembled FN nanonetworks on Ti/PEA activates growth factor receptors in concert integrins after cell attachment to FN network, and potentiates the response toward cell migration, proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Engineered coatings for titanium implants to present ultra-low doses of BMP-7

Al-Jarsha

Moulisová

Leal‐Egaña

et al. 2018

ACS Biomater. Sci. Eng.

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The ongoing research to improve the clinical outcome of titanium implants has resulted in the implemetation of multiple approches to deliver osteogenic growth factors accelerating and sustaining osseointegration. Here we show the presentation of human bone morphogenetic protein 7 (BMP-7) adsorbed to titanium discs coated with poly(ethyl acrylate) (PEA). We have previously shown that PEA promotes fibronectin organization into nanonetworks exposing integrin- and growth-factor-binding domains, allowing a synergistic interaction at the integrin/growth factor receptor level. Here, titanium discs were coated with PEA and fibronectin and then decorated with ng/mL doses of BMP-7. Human mesenchymal stem cells were used to investigate cellular responses on these functionalized microenvironments. Cell adhesion, proliferation, and mineralization, as well as osteogenic markers expression (osteopontin and osteocalcin) revealed the ability of the system to be more potent in osteodifferentiation of the mesenchymal cells than combinations of titanium and BMP-7 in absence of PEA coatings. This work represents a novel strategy to improve the biological activity of titanium implants with BMP-7.

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Effect of recombinant human bone morphogenetic protein‐7 (rhBMP‐7) on the viability, proliferation and differentiation of osteoblast‐like cells cultured on a chemically modified titanium surface

Cited by 8 publications

References 32 publications

Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

Enhancing the osteoblastic differentiation through nanoscale surface modifications

Engineered coatings for titanium implants to present ultra-low doses of BMP-7

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