Effect of radiation processing in elimination of Klebsiella pneumoniae from food

Gautam, Raj Kamal; Nagar, Vandan; Shashidhar, Ravindranath

doi:10.1016/j.radphyschem.2015.06.016

Cited by 17 publications

(6 citation statements)

References 25 publications

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“…Other study by Gautam [42].Find irradiation sensitivity of K. pneumoniae MTCC 109 was efficient of different food samples 1.5 kGy dose result elimination of K. pneumoniae from food samples (sprouts, poultry and fish) samples.…”

Section: Al-waelimentioning

confidence: 91%

Effects of some physical agents on Staphylococcus epidermidis and Leishmania tropica: An In vitro study

Al-Waeli

2018

ijs

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This work evaluated the effect of Alpha, Gamma irradiation and Nd:YAG, He-Ne laser on Staphylococcus epidermidis and Leishmania tropica in vitro. The experiment included five replicate of S. epidermidis , L. tropica in vitro exposed to effect of Alpha , Gamma irradiation by 241 Am isotopes , in two doses = 0.31993 MsV (Milli Sievert) for 3 hr, and in dose = 1.4157 MsV for 3hr , Americium isotopes give two type of decay Alpha and Gamma Rays. Effect of Nd:YAG laser in 1000 pulse between each pulse 6 sec, in wavelength 1.06 nm ; also He-Ne laser in 5, 10 , 20 , 30 minute , with wavelength 632.8nm. The effect of Nd:YAG, He-Ne laser and Alpha, Gamma irradiation on the viability of S. epidermidis , L. tropica in vitro was determined by using the specific equation compared with Control and determined by using colorimetric MTT assay respectively , the number of viable cells of S. epidermidis , L. tropica was fewer than control (without exposure to laser and irradiation). Nd:YAG laser, He-Ne laser and Alpha , Gamma irradiation was efficient to kill S. epidermidis and L. tropica .

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Section: Al-waelimentioning

confidence: 91%

Effects of some physical agents on Staphylococcus epidermidis and Leishmania tropica: An In vitro study

Al-Waeli

2018

ijs

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“…No reports are available for the radiation sensitivity on S. putrefaciens. But the D 10 values of gamma radiation on negative gram bacteria Escherichia coli O157:H7 in ground beef ranged from 0.241 to 0.307 kGy (Clavero et al 1994), and Klebsiella pneumoniae MTCC 109 in nutrient broth was 0.136 kGy (Gautam et al 2015). Statically analysis showed that cinnamon oil could significantly affected D 10 of gamma radiation (p \ 0.05).…”

Section: Mic D 10 and Rrsmentioning

confidence: 99%

Antimicrobial effect and mechanism of cinnamon oil and gamma radiation on Shewanella putrefaciens

et al. 2018

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The aims of this study were to observe the antimicrobial effect and mechanism of cinnamon oil combined with gamma radiation on Gamma radiation increased the antimicrobial activity of cinnamon oil, and the relative radiation sensitivity of gamma radiation on was increased by cinnamon oil. Gamma radiation significantly increased the changes of bacterial morphology, intra-adenosine 5'-triphosphate (intra-ATP) and extra-ATP concentrations and pH value of treated cinnamon oil. Although, gamma radiation used alone didn't damage the bacterial morphology and ATP concentrations significantly. Gamma radiation assisted cinnamon oil to damage the cell permeability and integrity of thus the combination of cinnamon oil and gamma radiation showed a better antimicrobial activity than used alone.

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“…This could be attributed to the variation in intrinsic and extrinsic parameters, such as pH, oxygen tension, protein composition, and bacterial rate of growth, which might influence the sensitivity of various bacteria in a specific medium (Thayer et al, 1995;Sommers and Boyd, 2006;Gomes et al, 2011). it has been reported that the radiation sensitivity of K. pneumoniae MTCC 109 was significantly greater (P < 0.05) in aqueous media than on sprout homogenate, poultry homogenate, and fish homogenate (Gautam et al, 2015). In that study, the radiation sensitivity of K. pneumoniae MTCC 109 was 0.116 kGy in saline, 0.136 kGy in nutrient broth, 0.142 kGy in sprout homogenate, 0.125 kGy in poultry homogenate, and 0.277 kGy in fish homogenate.…”

Section: Gautam Et Al (2015)mentioning

confidence: 99%

Gamma-irradiation induced effects on histamine-forming bacteria isolated from the chilled mackerel fish

AbdElAl¹

2022

Egypt. J. of Aquatic Biolo. and Fish.

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Scombroid food poisoning is caused by the consumption of histaminecontaining foods. Since histamine is thermally stable, controlling histamineforming bacteria in seafood is an effective technique for limiting histamine generation. The aim of this study was to investigate the induced effects of gamma radiation on the development of histamine-forming bacteria, specifically Pantoea agglumerance, in both mackerel flesh and tryptone soya broth. Klebsiella pneumoniae, Pseudomonas fluoresense, Pantoea agglumerance, and Bacillus sp. are histamine-forming bacteria were cultured in tryptone soy broth and exposed to gamma irradiation at doses of 0.25-5 kGy. In the meantime, mackerel meat was injected with the histamine-forming bacteria and then gamma-irradiated at 0.25-5.0 kGy. K. pneumonia and Pseudomonas fluoresense were particularly susceptible to gamma irradiation, and at 1.5 kGy, they were completely destroyed. Pantoea agglumerance and Bacillus sp. were found to be more resistant, with total inactivation at 3.0 and 5.0 kGy, respectively. The D 10values to inactivate 90% of the bacterial populations in tryptone soya broth and inoculated mackerel ranged from 0.13 to 1.03 kGy and 0.14 to 1.12 kGy, respectively, depending on the kind of bacteria. These findings imply that gamma irradiation can efficiently eradicate histamine-forming bacteria, lowering the risk of histamine poisoning from seafood. However, because modest doses of irradiation are ineffective in decreasing Bacillus sp, the early counts of Bacillus sp in fish samples should be closely monitored.

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Effect of radiation processing in elimination of Klebsiella pneumoniae from food

Cited by 17 publications

References 25 publications

Effects of some physical agents on Staphylococcus epidermidis and Leishmania tropica: An In vitro study

Effects of some physical agents on Staphylococcus epidermidis and Leishmania tropica: An In vitro study

Antimicrobial effect and mechanism of cinnamon oil and gamma radiation on Shewanella putrefaciens

Gamma-irradiation induced effects on histamine-forming bacteria isolated from the chilled mackerel fish

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