Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) are used to treat inflammation and pain and even to prevent the progression of cardiovascular disease. They have become widely used because of their effectiveness, especially among athletes performing high-intensity training. Indomethacin is used for pain management in sports medicine and is highly effective and versatile. However, several clinical studies have reported that indomethacin induces acute renal damage. In the present study, we determined that indomethacin reduced human embryonic kidney 293 (HEK293) cell viability in a concentration-dependent manner by triggering apoptosis. In addition, we demonstrated the effect of quercetin on indomethacin-treated HEK293 cells by inactivating the caspase-3 and caspase-9 signals. Furthermore, quercetin reduced ROS production and increased mitochondrial membrane potential (ΔΨm) in indomethacin-treated HEK293 cells. Our results indicate that quercetin can interrupt the activated caspase and mitochondrial pathway induced by indomethacin in HEK293 cells and affect apoptotic mRNA expression. Quercetin can protect against indomethacin-induced HEK293 cell apoptosis by regulating abnormal ΔΨm and apoptotic mRNA expression.

Effects of Tetrandrine on the Apoptosis of Cisplatin-resistant Oral Cancer Cells

“…The assay was executed in accordance with the protocol for the use of the Qiagen RNeasy Mini Kit, as described previously. 31, 32 RNA samples were processed with kit reagent for 30 min at 42°C in accordance with the instructions provided by the manufacturer (Applied Biosystems, Foster City, CA, USA). The following protocol was used in the subsequent qPCR: 2 min at 50°C, 10 min at 95°C, 40 15-s cycles at 95°C, and 1 min at 60°C with 1 µL of complementary reverse-transcribed DNA, 2X SYBR Green PCR Master Mix (Applied Biosystems), and forward and reverse primers (200 nM; Table 1).…”

Preclinical evidence of reno-protective effect of quercetin on acute kidney injury: a meta-analysis of animal studies

“…Quercetin attenuates cell injury and apoptosis in contrast-induced AKI models by inhibiting HIF-1α on the lncRNA NEAT1/HMGB1 signaling pathway (Luo et al, 2022). In indomethacin-induced AKI, quercetin protects HEK293 cells against cell apoptosis by reducing ROS production, increasing mitochondrial membrane potential, and down-regulating apoptotic expression of the caspase-3 and caspase-9 signals (Chen et al, 2021). Moreover, quercetin significantly suppressed the nephrotoxic effect of cisplatin by inhibiting tubular necrosis/apoptosis and the activity of the apoptosis executioner caspase-3 (Sanchez-Gonzalez et al, 2011).…”