Effect of Pseudomonas moorei KB4 Cells’ Immobilisation on Their Degradation Potential and Tolerance towards Paracetamol

Abstract

Pseudomonas moorei KB4 is capable of degrading paracetamol, but high concentrations of this drug may cause an accumulation of toxic metabolites. It is known that immobilisation can have a protective effect on bacterial cells; therefore, the toxicity and degradation rate of paracetamol by the immobilised strain KB4 were assessed. Strain KB4 was immobilised on a plant sponge. A toxicity assessment was performed by measuring the concentration of ATP using the colony-forming unit (CFU) method. The kinetic parameters of paracetamol degradation were estimated using the Hill equation. Toxicity analysis showed a protective effect of the carrier at low concentrations of paracetamol. Moreover, a pronounced phenomenon of hormesis was observed in the immobilised systems. The obtained kinetic parameters and the course of the kinetic curves clearly indicate a decrease in the degradation activity of cells after their immobilisation. There was a delay in degradation in the systems with free cells without glucose and immobilised cells with glucose. However, it was demonstrated that the immobilised systems can degrade at least ten succeeding cycles of 20 mg/L paracetamol degradation. The obtained results indicate that the immobilised strain may become a useful tool in the process of paracetamol degradation.

Characterization and biodegradation of paracetamol by biomass of Bacillus licheniformis strain PPY-2 isolated from wastewater

“…Additionally, the degradation is influenced by many environmental factors such as pH, temperature, and heavy metals. Also, when degradation is mediated by immobilized cells of Pseudomonas moorei KB4, by this strain, the degradation rate is enhanced (Surma et al 2021 ).…”

Application of Immobilized Biocatalysts in the Biotransformation of Non-Steroidal Anti-Inflammatory Drugs

“…Planococcus sp. S5, Bacillus thuringiensis B1(2015b), Pseudomonas moorei KB4, and Stenotrophomonas maltophilia KB2 were immobilized separately through the adsorption on the surface of the loofah sponge, according to Dzionek et al [20], Dzionek et al [21], Surma et al [22], Wojcieszy ńska et al [23], respectively. The initial optical density of the cultures used during the immobilization of Planococcus sp.…”

“…In previous studies, the authors successfully immobilized strains capable of degrading selected NSAIDs on the plant sponge Luffa cylindrica and characterized their properties [20][21][22][23]. The choice of plant sponge from Loofah cylindrica was dictated by the fact that it is a cheap, biodegradable, and biocompatible material with a hydrophilic surface on which there are many functional groups.…”

Draft Genomic Sequences of Pseudomonas moorei Strains Isolated from Wild Cranberry Bogs in Massachusetts

“… Pseudomonas moorei , first isolated from Elbe river sediments in Germany, is notable for its ability to detoxify pharmaceutical industry environmental contaminants such as 4- and 5-chlorosalicylates, acetaminophen, and diclofenac ( 1 – 3 ), but nothing is known about the activities of these bacteria in pristine ecosystems. Two P. moorei strains were isolated from dune swale bog soils as part of a culture-dependent survey of bacteria within the Cape Cod National Seashore (42.070624 N, 70.210548 W and 42.064742 N, 70.117562 W) in early July 2012.…”