Effect of postconditioning and atorvastatin in preventing remote intestinal reperfusion injury

Abstract

Objective To evaluate the capacity of ischemic postconditioning and atorvastatin in prevent or minimize reperfusion injury in small bowel of rats subjected to ischemia and reperfusion by abdominal aorta clamping. Methods 41 Wistar norvegic rats were distributed into 5 groups: ischemia and reperfusion, ischemic postconditioning, postconditioning + statin, statin and Sham. After anesthesia, laparotomy and dissection of the infra-renal abdominal aorta were performed; except the Sham group, all others were subjected to aorta clamping for 70 min (ischemia) and withdrawal of clamp for 70 min (reperfusion). In the IPC and IPC + S groups, four cycles of postconditioning were performed between the phases of ischemia and reperfusion lasting 30 s each. In IPC + S and S groups, 3.4 mg/day of atorvastatin was given for seven days per gavage; 1 cm of the ileum were removed for histological study and the results were subjected to statistical treatment considering significant p < 0.05. Results The average of intestinal lesion was 2 in the I/R group, 0.66 in the IPC group, 0 in the IPC + S group, 0 in the S group, and 0 in the SHAM group. Conclusion The ischemic postconditioning and atorvastatin were capable of minimizing intestinal reperfusion injury, either alone or in combination.

Ischaemic postconditioning reduces apoptosis in experimental jejunal ischaemia in horses

“…Most research assessing the effect of IPoC on intestinal ischaemia/reperfusion (I/R) injury, has been performed in laboratory animals by use of an experimental model occluding the cranial mesenteric artery. Many different variables for intestinal ischaemia reperfusion injury have been investigated in these studies, where intestinal IPoC was most commonly associated with decreased histomorphological damage [ 13 – 21 ]. Moreover, reduced apoptosis, less oxidative stress and neutrophil activation, as well as higher superoxide dismutase or glutathion levels have been reported in groups subjected to IPoC [ 14 – 21 ].…”