Effect of polyethylene glycol on preparation of rifampicin-loaded PLGA microspheres with membrane emulsification technique

Ito, Fuminori; Fujimori, Hiroyuki; Honnami, Hiroyuki; Kawakami, Hiroyoshi; Kanamura, Kiyoshi; Makino, Kimiko

doi:10.1016/j.colsurfb.2008.05.011

Cited by 50 publications

(37 citation statements)

References 13 publications

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“…In particulate form (both micro and nano), several polymeric systems have been used for the delivery of drugs and therapeutic proteins (Blasi et al 2007;Silva et al 2006;Ito et al 2008;Naha et al 2008 and2009a). Given their potential widespread use, there is an increasing need for information regarding the human health and environmental implications of these polymeric nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Intracellular localisation, geno- and cytotoxic response of polyN-isopropylacrylamide (PNIPAM) nanoparticles to human keratinocyte (HaCaT) and colon cells (SW 480)

et al. 2010

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PNIPAM nanoparticles, with and without a covalently-linked fluorescent label, were prepared by a free radical polymerisation technique. The cyto-and genotoxicity of PNIPAM nanoparticles were analysed in two representative mammalian cell lines, SW480, a colon, and HaCaT, a dermal cell line. Physical characterisation in terms of particle size and zeta potential of the PNIPAM nanoparticles was carried out both in aqueous solution and in the appropriate cell culture media. Uptake and co-localisation of fluorescently labelled PNIPAM nanoparticles was monitored in both cell lines using confocal laser scanning microscopy. Genotoxicity analysis using the Comet assay was performed in both cell lines to evaluate any DNA damage. It was observed that the PNIPAM nanoparticles were internalized and localised in lysosomes within 24 hrs. No significant cytotoxic response (p ≤ 0.05) was observed in either cell line over concentration ranges from 25 mg/l to 1000 mg/l for all exposure time periods.Furthermore, no significant genotoxic response (p ≤ 0.05) was observed in either cell line over concentration ranges from 12.5 mg/l to 800 mg/l for all exposure time periods. The results suggest that the PNIPAM nanoparticles show excellent biocompatibility in vitro.

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Section: Introductionmentioning

confidence: 99%

Intracellular localisation, geno- and cytotoxic response of polyN-isopropylacrylamide (PNIPAM) nanoparticles to human keratinocyte (HaCaT) and colon cells (SW 480)

et al. 2010

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“…In particular polylactic-co-glycolic acid (PLGA) and poly-lactic acid (PLA) polymers, which are biodegradable in nature, show great potential and have been widely used in drug delivery systems (Ito et al, 2008;Naha et al, 2008a,b;Sun et al, 2008). Currently four other nanoparticles namely, polymer coated iron oxide nanoparticles, PEGylated liposomes, dendritic fullerenes, and fullerene derivative nanoparticles are being investigated in clinical trials for their anticancer activity (Dobrovolskaia and McNeil, 2007).…”

Section: Introductionmentioning

confidence: 99%

Preparation, characterization of NIPAM and NIPAM/BAM copolymer nanoparticles and their acute toxicity testing using an aquatic test battery

Naha¹,

Casey²,

Tenuta

et al. 2009

Aquatic Toxicology

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“…[23][24][25][26] Due to this plasticizer activity, PEG 600 and PEG 6000 were used in this experiment and they retarded release of NS Figure 1. SEM photograph of Ethocel 20cps microspheres of NS containing 40% of TEC at three different magnifications significantly specially at 40% level.…”

Section: Resultsmentioning

confidence: 99%

Study of Ethyl Cellulose Based Sustained Release Microspheres of Naproxen Sodium

Sarkar

Sadat

Islam

et al. 2012

Dhaka Univ. J. Pharm. Sci

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The present study was conducted to prepare sustained release microspheres of naproxen sodium using ethyl cellulose (Ethocel 20 cps) polymer. Three different plasticizers namely polyethyleneglycol 600 (PEG 600), polyethyleneglycol 6000 (PEG 6000) and triethyl citrate (TEC) were used at 10% (wt/wt) and 40% (wt/wt) level of the drug content. Prepared microspheres were characterized with respect to drug loading, microsphere particle size, microsphere surface morphology and release behavior. Surface morphology of the microspheres was examined in a scanning electron microscope. Drug release was observed in phosphate buffer solution of pH 6.8 for 8 hours. At 10% level of the plasticizers, percent release was 93.36%, 93.02% and 92.67% for PEG 600, PEG 6000 and TEC respectively after 8 hours. On the other hand, at 40% level, percent release was 81.12%, 70.06% and 51.12% for PEG 600, PEG 6000 and TEC respectively after the same duration. Release mechanisms followed case I or fickian model.

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Effect of polyethylene glycol on preparation of rifampicin-loaded PLGA microspheres with membrane emulsification technique

Cited by 50 publications

References 13 publications

Intracellular localisation, geno- and cytotoxic response of polyN-isopropylacrylamide (PNIPAM) nanoparticles to human keratinocyte (HaCaT) and colon cells (SW 480)

Intracellular localisation, geno- and cytotoxic response of polyN-isopropylacrylamide (PNIPAM) nanoparticles to human keratinocyte (HaCaT) and colon cells (SW 480)

Preparation, characterization of NIPAM and NIPAM/BAM copolymer nanoparticles and their acute toxicity testing using an aquatic test battery

Study of Ethyl Cellulose Based Sustained Release Microspheres of Naproxen Sodium

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