Effect of pollutants on the ergosterol content as indicator of fungal biomass

Barajas-Aceves, Martha; Hassan, Mainul; Tinoco, Raunel; Vázquez-Duhalt, Rafael

doi:10.1016/s0167-7012(02)00031-3

Cited by 77 publications

(39 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results are reasonable when compared with the results e.g. Pasanen et al (1999), Barajas-Aceves et al (2002) and Eikenes et al (2005) from 2 to 16 lg g -1 .…”

Section: Discussionsupporting

confidence: 92%

Demethoxylation of [O14CH3]-labelled lignin model compounds by the brown-rot fungi Gloeophyllum trabeum and Poria (Postia) placenta

2007

View full text Add to dashboard Cite

Demethoxylation reactions in the cultures of the brown-rot fungi Gloeophyllum trabeum and Poria placenta were studied by determining the evolution of (14)CO(2) from a non-phenolic lignin model, beta-O-4 dimer, [O(14)CH(3)]-labelled at position 4 in the A ring (model I), and from [O(14)CH(3)]-labelled vanillic acid (model II). The fungi were grown under oxygen or air atmosphere on an agar medium with or without spruce sapwood blocks. The dimeric model (I) was impregnated onto agar or wood block in cultures to clarify the possible effect of wood as growth substrate. In the case of vanillic acid (model II), birch wood was used. The effect of supplemented nutrient nitrogen (2 mM N) and glucose (0.1 or 1.0% w/v) on demethoxylation was also studied. G. trabeum enhanced the production of (14)CO(2) from the dimer in the presence of spruce wood blocks. It released (14)CO(2) from the methoxyl groups giving 30-60% of the applied activity in 8 weeks. P. placenta produced almost 30% (14)CO(2 )from vanillic acid (model II) in 9 weeks under oxygen, but from the methoxyl group of the dimer only 3% of (14)CO(2) was evolved in 4 weeks. The biomasses determined as ergosterol assay showed variation from 14 to 226 microg g(-1) dry weight of agar, and 2 to 9 microg g(-1 )of wood, but they did not correlate with the production of (14)CO(2). The results indicate that these brown-rot fungi possess different mechanisms for demethoxylation.

show abstract

“…Our results are reasonable when compared with the results e.g. Pasanen et al (1999), Barajas-Aceves et al (2002) and Eikenes et al (2005) from 2 to 16 lg g -1 .…”

Section: Discussionsupporting

confidence: 92%

Demethoxylation of [O14CH3]-labelled lignin model compounds by the brown-rot fungi Gloeophyllum trabeum and Poria (Postia) placenta

2007

View full text Add to dashboard Cite

show abstract

“…However, in the presence of Spirulina platensis PC extracts, there was no significant inhibition (95% confidence) of the ergosterol content produced throughout the period of the study. This fact suggests that the ergosterol might not be a good indicator to monitor the Aspergillus flavus biomass development because it remained relatively constant in the control and in the culture with PC, indicating that they did not affect the membrane components, as was mentioned by Behalová et al(1994), Barajas-Aceves et al (2002), Gutarowska and Zakowska, (2009). Figure 2 shows the percentage of fungal inhibition by the phenolic extract from Spirulina platensis in each component under evaluation.…”

Section: Determination Of the Glucosaminementioning

confidence: 92%

Assessment of the antifungal activity of Spirulina platensis phenolic extract against Aspergillus flavus

et al. 2011

View full text Add to dashboard Cite

The production of safe food has stimulated the search for natural substances that possess antifungal activity. The indirect methods of estimating fungal biomass are based on the measurement of glucosamine, ergosterol and protein -typical compounds produced during the development of biomass. The aim of the study was to assess the effect of the phenolic extract from Spirulina platensis on the production of structural compounds in Aspergillus flavus, in order to identify its action on fungal inhibition. The Spirulina platensis methanolic extracts presented 1.15 mg phenolic compound/g Spirulina platensis, which showed an antifungal effect against Aspergillus flavus, inhibiting the glucosamine production up to 56%. Therefore, it may be employed as natural defense when food protection is necessary.Index terms: Phenolic inhibitors, antifungal property, glucosamine, protein, ergosterol. RESUMOA produção de alimentos seguros tem estimulado a busca por substâncias naturais que possuem atividade antifúngica. Os métodos indiretos de estimativa de biomassa fúngica são baseados na medição de glucosamina, ergosterol e proteína -compostos típicos produzidos durante o desenvolvimento da biomassa. Neste estudo, objetivou-se avaliar o efeito do extrato fenólico de Spirulina platensis na produção de componentes estruturais em Aspergillus flavus, a fim de identificar seu mecanismo de ação dos fenóis na inibição fúngica. O extrato metanólico de Spirulina platensis apresentou 1,15 mg de compostos fenólicos/g Spirulina platensis, apresentando um efeito antifúngico contra Aspergillus flavus, inibindo a produção de glucosamina em até 56%. Portanto, pode ser empregado como antifúngico natural quando for necessária a proteção de alimentos.

show abstract

“…The initial time denotes the moment of mixing the sludge with the fungal inoculum. The ergosterol content, a specific component of fungal cell membranes, was determined as a biomarker of viable mycelium, and therefore of fungal growth (Barajas-Aceves et al, 2002). Ergosterol profiles in Fig.…”

Section: Growth and Activity Of T Versicolor In The Solid-phase Sludmentioning

confidence: 99%

Solid-phase treatment with the fungus Trametes versicolor substantially reduces pharmaceutical concentrations and toxicity from sewage sludge

Rodríguez-Rodríguez

Jelić

Llorca

et al. 2011

Bioresource Technology

View full text Add to dashboard Cite

Effect of pollutants on the ergosterol content as indicator of fungal biomass

Cited by 77 publications

References 33 publications

Demethoxylation of [O14CH3]-labelled lignin model compounds by the brown-rot fungi Gloeophyllum trabeum and Poria (Postia) placenta

Demethoxylation of [O14CH3]-labelled lignin model compounds by the brown-rot fungi Gloeophyllum trabeum and Poria (Postia) placenta

Assessment of the antifungal activity of Spirulina platensis phenolic extract against Aspergillus flavus

Solid-phase treatment with the fungus Trametes versicolor substantially reduces pharmaceutical concentrations and toxicity from sewage sludge

Contact Info

Product

Resources

About