Effect of plant elicitors on the reproduction of the root-knot nematode Meloidogyne chitwoodi on susceptible hosts

Santos, Maria Clara Vieira dos; Curtis, R. H. C.; Abrantes, Isabel

doi:10.1007/s10658-012-0155-6

Cited by 24 publications

(25 citation statements)

References 24 publications

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“…Also Ithal et al (2007 b ) detected a strong and consistent, but very local, suppression of the jasmonic acid pathway in isolated syncytia after cyst nematode infection in soybean. It is important to note that activation of the jasmonic acid pathway by external methyl jasmonate application is an effective way to protect potato, tomato, and rice from RKN infection (Cooper et al , 2005; Nahar et al , 2011; Vieira dos Santos et al , 2013). …”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis through RNA sequencing of giant cells induced by Meloidogyne graminicola in rice roots

Gheysen

Denil

et al. 2013

Journal of Experimental Botany

120

123

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One of the reasons for the progressive yield decline observed in aerobic rice production is the rapid build-up of populations of the rice root knot nematode Meloidogyne graminicola. These nematodes induce specialized feeding cells inside root tissue, called giant cells. By injecting effectors in and sipping metabolites out of these cells, they reprogramme normal cell development and deprive the plant of its nutrients. In this research we have studied the transcriptome of giant cells in rice, after isolation of these cells by laser-capture microdissection. The expression profiles revealed a general induction of primary metabolism inside the giant cells. Although the roots were shielded from light induction, we detected a remarkable induction of genes involved in chloroplast biogenesis and tetrapyrrole synthesis. The presence of chloroplast-like structures inside these dark-grown cells was confirmed by confocal microscopy. On the other hand, genes involved in secondary metabolism and more specifically, the majority of defence-related genes were strongly suppressed in the giant cells. In addition, significant induction of transcripts involved in epigenetic processes was detected inside these cells 7 days after infection.

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Section: Discussionmentioning

confidence: 99%

Transcriptional analysis through RNA sequencing of giant cells induced by Meloidogyne graminicola in rice roots

Gheysen

Denil

et al. 2013

Journal of Experimental Botany

120

123

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“…Of the two BABA concentrations evaluated, we found that the most effective treatment overall was 20mM BABA. It is interesting to note that several studies have noted that 20 mM (not less or more) is the most effective concentration of BABA to induce resistance against a wide variety of pathogens including Penicillium digitatum, Phytophthora infestans and root knot nematode (Porat et al, 2003;Oka et al, 1999Cohen et al, 2001 (Nandi et al, 2003;Chinnasri et al, 2006;Vieira dos Santos et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…incognita and M. chitwoodi (Ge et al, 1999;Nandi et al, 2003;Altamiranda et al, 2008;Olivieri et al, 2009;dos Santos et al, 2013;Moahmed et al, 2013). However, there are hardly any studies investigating the effect of BABA against complexes of these phytopathogens.…”

Section: Discussionmentioning

confidence: 99%

“…Elicitor induced resistance has not been widely explored for control of RKN although there are several reports (Oka et al, 1999;Oka and Cohen 2001;Nandi et al, 2003;Channasri et al, 2006;Vieira dos Santos et al, 2013). However, there are hardly any studies investigating the effect of elicitors against RKN as a primary pathogen in conjunction with a secondary pathogen.…”

Section: Introductionmentioning

confidence: 99%

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The effect of β-aminobutyric acid (BABA) on root knot nematode and soft rot pathogen disease complexes in Solanum tuberosum plants

Mongae

Moleleki

2015

Eur J Plant Pathol

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The efficacy of three elicitors, DL-β-aminobutyric acid (BABA), Acibenzolar-S-methyl (Bion) and Messenger (Harpin) on potato plants inoculated with Meloidogyne incognita second stage juveniles (J2s) was investigated in this study. The efficacy of each elicitor was evaluated based on galling index, egg numbers, total root knot nematode (RKN) population numbers, number of juveniles and the percentage of females in respective extracted populations per treatment at 28 and 56 dpi. There was a significant decrease in galling indices of all treated compared to untreated control plants. The highest percentage reduction was observed for 20 mM BABA-treated plants (33%) followed by 5mM BABA (23%), 50mg/L Bion (18%), 100mg/L Bion (15%) and Messenger (8%). The number of eggs extracted from plants treated with 20mM BABA was significantly lower (66%) compared to untreated controls. Furthermore, treating plants with 20mM BABA decreased the total RKN population numbers by 55% whilst the decrease observed for 2 Bion and Messenger was between 24-39%. Of the three tested elicitors, 20mM BABA was found to be the most effective elicitor at both 28 and 56 dpi. Hence, it was selected for further testing against RKN and Pectobacterium carotovorum subsp. brasiliense (Pcb) disease complex. Efficacy was tested by comparing the colony forming units (cfu) of Pcb from treated and untreated control plants inoculated with M. incongita and Pcb simultaneously. The concentration of Pcb from untreated plants inoculated with both pathogens was significantly higher (3.3 × 10 3 cfu/ml) compared to their treated counterparts (8.71 × 10 2 cfu/ml). This suggests that BABA has the potential to decrease soft rot bacterial infections in RKN disease complexes by decreasing the rate of RKN infection. This is the first report where BABA is shown to decrease the infection rate of RKN as a primary causal agent in combination with a secondary pathogen.

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“…chitwoodi co-cultures Meloidogyne chitwoodi (CRKN) egg masses were handpicked from infected tomato plants as described by Vieira dos Santos et al (2013). The eggs were extracted with 0.52 % (v/v) sodium hypochlorite (NaOCl) solution (Hussey and Barker 1973) and the debris removed with a 47 % (w/v) sucrose solution according to McClure et al (1973).…”

Section: Establishment Of Solanum Tuberosum Hairy Root Culturesmentioning

confidence: 99%

In vitro co-culture of Solanum tuberosum hairy roots with Meloidogyne chitwoodi: structure, growth and production of volatiles

Faria

Sena

Maleita

et al. 2014

Plant Cell Tiss Organ Cult

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Meloidogyne spp., commonly known as rootknot nematodes (RKNs), are economically important plant sedentary endoparasites that cause galls on susceptible hosts. The Columbia root-knot nematode (CRKN), M. chitwoodi, is a quarantine A2 type pest by the European and Mediterranean Plant Protection Organization since 1998. This nematode has been found associated with economically important crops such as potato and tomato, causing severe damage and making the agricultural products unacceptable for the fresh market and food processing. In vitro co-culture of host and parasite offers an advantageous experimental system for studying plant-RKN interactions. The structure, growth and production of volatiles of Solanum tuberosum hairy roots (HR) and of S. tuberosum HR/ CRKN co-cultures were compared. HR were induced by inoculation of aseptic potato tuber segments with Rhizobium rhizogenes. Co-cultures were initiated by inoculating HR with sterilized CRKN eggs. Infection with CRKN induced the RKN symptomatology in the HR and several nematode life stages were observed by light and scanning electron microscopy. Potato HR and HR/CRKN co-cultures exhibited similar growth patterns, evaluated by measuring fresh and dry weight and by the dissimilation method. Volatiles, isolated by distillation-extraction and analyzed by gas chromatography (GC) and gas chromatography coupled to mass spectrometry, revealed that palmitic acid (37-52 %), n-pentadecanal (10-16 %) and linoleic acid (2-16 %) were the main constitutive components of S. tuberosum HR, and of the HR/CRKN co-cultures (24-44, 8-22 and 4-18 %, respectively

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Effect of plant elicitors on the reproduction of the root-knot nematode Meloidogyne chitwoodi on susceptible hosts

Cited by 24 publications

References 24 publications

Transcriptional analysis through RNA sequencing of giant cells induced by Meloidogyne graminicola in rice roots

Transcriptional analysis through RNA sequencing of giant cells induced by Meloidogyne graminicola in rice roots

The effect of β-aminobutyric acid (BABA) on root knot nematode and soft rot pathogen disease complexes in Solanum tuberosum plants

In vitro co-culture of Solanum tuberosum hairy roots with Meloidogyne chitwoodi: structure, growth and production of volatiles

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