Effect of phosphate glass reinforcement on the mechanical and biological properties of freeze-dried gelatin composite scaffolds for bone tissue engineering applications

Govindan, R.; Gu, Feng; Karthi, S.; Girija, E. K.

doi:10.1016/j.mtcomm.2019.100765

Cited by 22 publications

(19 citation statements)

References 71 publications

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“…The hydrolytic degradation of PG releases Ca, Mg, and P that facilitate the deposition of hydroxyapatite on the surface of scaffolds to induce the osteoconductivity [ 15 ]. The presence of PG was reported to enhance differentiation of MG-63 osteoblast-like cells, while a PG/gelatin scaffold was found with comprehensive improvements of viability and proliferation profile of MG-63 cells and in vitro bioactivity over the gelatin-only counterparts, owing to the release of Ca, Mg, and P from the PG [ 16 , 17 , 18 ]. Recently, the PG/PLA composites were successfully fabricated via fused deposition modelling (FDM).…”

Section: Introductionmentioning

confidence: 99%

Additive-Manufactured Gyroid Scaffolds of Magnesium Oxide, Phosphate Glass Fiber and Polylactic Acid Composite for Bone Tissue Engineering

Liu

Rudd

2021

Polymers

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Composites of biodegradable phosphate glass fiber and polylactic acid (PGF/PLA) show potential for bone tissue engineering scaffolds, due to their ability to release Ca, P, and Mg during degradation, thus promoting the bone repair. Nevertheless, glass degradation tends to acidify the surrounding aqueous environment, which may adversely affect the viability and bone-forming activities of osteoblasts. In this work, MgO was investigated as a neutralizing agent. Porous network-phase gyroid scaffolds were additive-manufactured using four different materials: PLA, MgO/PLA, PGF/PLA, and (MgO + PGF)/PLA. The addition of PGF enhanced compressive properties of scaffolds, and the resultant scaffolds were comparably strong and stiff with human trabecular bone. While the degradation of PGF/PLA composite induced considerable acidity in degradation media and intensified the degradation of PGF in return, the degradation media of (MgO + PGF)/PLA maintained a neutral pH close to a physiological environment. The experiment results indicated the possible mechanism of MgO as the neutralizing agent: the local acidity was buffered as the MgO reacted with the acidic degradation products thereby inhibiting the degradation of PGF from being intensified in an acidic environment. The (MgO + PGF)/PLA composite scaffold appears to be a candidate for bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Additive-Manufactured Gyroid Scaffolds of Magnesium Oxide, Phosphate Glass Fiber and Polylactic Acid Composite for Bone Tissue Engineering

Liu

Rudd

2021

Polymers

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“…These results indicated that water swelling and weight loss increased with increasing submersion time that allowed water molecules to easily diffuse into the scaffolds, leading to increased water swelling and weight loss. Moreover, incorporation of AC/HPβCD complex into the scaffolds decreased water swelling and weight loss of the pure Gel scaffold caused by greater interaction between the AC/HPβCD complex and the Gel resulting in hindering of interaction between Gel and water molecules 35 …”

Section: Resultsmentioning

confidence: 99%

Gelatin scaffolds loaded with asiaticoside/2‐hydroxypropyl‐β‐cyclodextrin complex for use as wound dressings

Choipang

Buntum

Chuysinuan

et al. 2020

Polymers for Advanced Techs

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Gelatin (Gel) is a protein polymer obtained from the hydrolysis of collagen. Gel was used to prepare a wound dressing material loaded with asiaticoside (AC), a trisaccharide triterpene from Centella asiatica (L.) Urban commonly used in wound healing and dermis reconstruction. To improve water solubility, AC was formed into an inclusion complexation with 2‐hydroxypropyl‐β‐cyclodextrin (HPβCD) in molar ratios of 1:0.5, 1:1, and 1:2. The AC/HPβCD complexes were then loaded into a standard Gel solution and glutaraldehyde was used as the crosslinking of the Gel matrix. Freeze‐drying was used to fabricate the mixed solutions into Gel scaffolds. Average pore sizes of the scaffolds ranged between 548 and 560 μm. The compressive modulus of the scaffolds increased with increasing HPβCD molar ratio. Swelling and weight loss of the scaffolds in water increased with longer submersion times. Highest cumulative released amount of AC was obtained from the scaffold incorporated with the AC/HPβCD complex at the molar ratio of 1:2. Indirect cytotoxicity assay with human dermal fibroblasts confirmed that AC/HPβCD complex‐loaded Gel scaffolds were non‐toxic to the cells. Results demonstrated the potential of AC/HPβCD complex‐loaded Gel scaffolds for use as wound dressing materials.

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“…Sharifi et al (2020) fabricated gelatin/chondroitin sulfate/polycaprolactone-based nanofibrous scaffolds and has shown better human mesenchymal stem cells (hMSCs) attachment and chondrogenesis differentiation for cartilage tissue engineering applications [40]. The ciprofloxacin-loaded three-dimensional porous phosphate glass-reinforced gelatin scaffolds for bone tissue engineering were synthesized; the scaffolds mimic ECM properties, and sustained release of ciprofloxacin was observed in the phosphate-buffered saline at 37 • C. Cell culture studies for these scaffolds were performed, and the results revealed good cell adhesion and proliferation with enhanced cell viability in osteoblast like MG-63 cells [41].…”

Section: Natural Polymer-based Biomaterialsmentioning

confidence: 99%

“…The ciprofloxacin-loaded three-dimensional porous phosphate glass-reinforced gelatin scaffolds for bone tissue engineering were synthesized; the scaffolds mimic ECM properties, and sustained release of ciprofloxacin was observed in the phosphate-buffered saline at 37 °C. Cell culture studies for these scaffolds were performed, and the results revealed good cell adhesion and proliferation with enhanced cell viability in osteoblast like MG-63 cells [ 41 ].…”

Section: Biomaterials For Tissue Engineeringmentioning

confidence: 99%

Advancement of Nanobiomaterials to Deliver Natural Compounds for Tissue Engineering Applications

Kumar

Abrahamse

2020

IJMS

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Recent advancement in nanotechnology has provided a wide range of benefits in the biological sciences, especially in the field of tissue engineering and wound healing. Nanotechnology provides an easy process for designing nanocarrier-based biomaterials for the purpose and specific needs of tissue engineering applications. Naturally available medicinal compounds have unique clinical benefits, which can be incorporated into nanobiomaterials and enhance their applications in tissue engineering. The choice of using natural compounds in tissue engineering improves treatment modalities and can deal with side effects associated with synthetic drugs. In this review article, we focus on advances in the use of nanobiomaterials to deliver naturally available medicinal compounds for tissue engineering application, including the types of biomaterials, the potential role of nanocarriers, and the various effects of naturally available medicinal compounds incorporated scaffolds in tissue engineering.

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Effect of phosphate glass reinforcement on the mechanical and biological properties of freeze-dried gelatin composite scaffolds for bone tissue engineering applications

Cited by 22 publications

References 71 publications

Additive-Manufactured Gyroid Scaffolds of Magnesium Oxide, Phosphate Glass Fiber and Polylactic Acid Composite for Bone Tissue Engineering

Additive-Manufactured Gyroid Scaffolds of Magnesium Oxide, Phosphate Glass Fiber and Polylactic Acid Composite for Bone Tissue Engineering

Gelatin scaffolds loaded with asiaticoside/2‐hydroxypropyl‐β‐cyclodextrin complex for use as wound dressings

Advancement of Nanobiomaterials to Deliver Natural Compounds for Tissue Engineering Applications

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