Effect of Peptide Semax on Synaptic Activity and Short-Term Plasticity of Glutamatergic Synapses of Co-Cultured Dorsal Root Ganglion and Dorsal Horn Neurons

Shypshyna, MS; Veselovsky, N. S.; Myasoedov, N. F.; Shram, S. I.; Fedulova, S. A.

doi:10.15407/fz61.04.048

Cited by 4 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the pain threshold is lowered, indicating that it is a successful model. For neuropathic pain, both cell electrophysiology and immunohistochemistry showed significantly increased P2X2 receptor expression, indicating that the neuropathic pain is closely related to the P2X2 receptor expression 24–26 .…”

Section: Discussionmentioning

confidence: 98%

“…P2X2/3 subtypes use primary sensory neurons while transmitting the nociception and algesia information 22,23 . The selective P2X2/3 and P2X3 receptor antagonists, in experimental pain models, effectively reduce neuropathic pain 24–26 . The CCI rats behave analogously with the neuropathic pain conditions of human beings, which were therefore used as the neuropathic pain model 27 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of 1,8-cineole on neuropathic pain mediated by P2X2 receptor in the spinal cord dorsal horn

Zheng

Zhang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

As an intractable health threat, neuropathic pain is now a key problem in clinical therapy, which can be caused by lesions affecting the peripheral nervous systems. 1,8-cineole is a natural monoterpene cyclic ether present in eucalyptus and has been reported to exhibit anti-inflammatory and antioxidant effects. Research has shown that 1,8-cineole inhibits P2X3 receptor-mediated neuropathic pains in dorsal root ganglion. The P2X2 and P2X3 receptors participate in the transmission of algesia and nociception information by primary sensory neurons. In the present study, We thus investigated in the spinal cord dorsal horn whether 1,8-cineole inhibits the expression of P2X2 receptor-mediated neuropathic pain. This study used rats in five random groups: group of chronic constriction injury(CCI) with dimethysulfoxide control (CCI + DMSO); group of CCI; sham group(Sham); group of CCI treated with a low dose 1,8-cineole (CCI + 50 mg/kg); group of CCI with a high dose (CCI + 100 mg/kg). We observed the effects of 1,8-cineole on thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT). We examined P2X2 receptors mRNA change in rat spinal cord dorsal horn by In situ nucleic acid hybridization(ISH) and Quantitative realtime polymerase chain reaction (qRT-PCR) methods. Western Blotting and Immunohistochemical staining methods were used to observe P2X2 receptor protein expressions in the rat spinal cord dorsal horn. It demonstrated that oral administration of 1,8-cineole inhibits over-expression of P2X2 receptor protein and mRNA in the spinal cord and dorsal horn in the CCI rats. And the study explored new methods for the prevention and treatment of neuropathic pain.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Effects of 1,8-cineole on neuropathic pain mediated by P2X2 receptor in the spinal cord dorsal horn

Zheng

Zhang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Therapeutic Semax peptide with primary sequence Met-Glu-His-Phe-Pro-Gly-Pro [34] was selected in present chapter as the first peptide for study. Semax is one of the few synthetic regulatory peptides that, after all the fundamental research, have found its application in therapy as a nootropic and neuroprotective agent [35].…”

Section: Semax Peptidementioning

confidence: 99%

Lysine Dendrimers and Their Complexes with Therapeutic and Amyloid Peptides: Computer Simulation

Попова¹,

Khamidova²,

Neelov³

et al. 2018

Dendrimers - Fundamentals and Applications

View full text Add to dashboard Cite

Lysine dendrimers consist of natural lysine amino acid residues. Due to this reason, they are usually not as toxic as other dendrimers. Lysine dendrimers are often used in drug and gene delivery. These dendrimers penetrate blood-brain barrier and thus could be used for the delivery of drugs and other substances, for example, bioactive peptides to brain or elimination of disease-related peptides out of the brain. To do it, dendrimers should form complex with these peptides. In the present chapter, we describe computer simulation of the interaction of lysine dendrimer of the second generation with three different peptides and check does it form complexes with them. Two of these peptides (Semax and Epithalon) are nootropic peptides and third is the fragment of amyloid peptide, which forms amyloid fibrils and plaques in Alzheimer's disease. Our simulation demonstrates that the lysine dendrimers form complexes with these therapeutics peptides. Thus, we demonstrated that lysine dendrimer is a good candidate for the delivery of therapeutic peptides. We also have shown that lysine dendrimer destroys existing stacks of amyloid peptides and forms a stable complex with them. Thus, it looks that it could be used in future for the treatment of Alzheimer's diseases.

show abstract

“…Due to this ability to make complexes the dendrigrafts like other dendritic molecules could be used as multifunctional nanocarriers for delivery of drug or/and other molecules [9] for treatment of various disease. Therapeutic Semax peptide [10] was selected for our study as a model peptide because it belongs to a class of regulatory peptides and has an antioxidant, antihypoxic and neuroprotective properties. Semax peptide is used for acute ischemic stroke prevention, during traumatic brain injury treatment, recovery of a patient after a stroke, in the case of optic nerve disease and glaucoma optic neuropathy.…”

Section: Introductionmentioning

confidence: 99%

Molecular Dynamics Simulation of Interaction of Short Lysine Brush and Oppositely Charged Semax Peptides

Попова¹,

Okrugin²,

Neelov³

2016

View full text Add to dashboard Cite

The possibility of complex formation by short lysine brush and therapeutic Semax peptides was investigated using molecular dynamics method. Lysine dendrimers and polymer brushes are used for drug and other (e.g., DNA, peptides, and polysaccharides) molecules delivery to different target cells. It is known that they could penetrate blood brain barrier. Since short lysine brush is nontoxic, a system containing of such brush and 8 oppositely charged Semax peptides was studied. It was obtained that stable complexes consisting of brush and peptides formed and structures of these complexes were investigated. Such complex can be used in future for delivery of Semax peptides to brain since these peptides have significant antioxidant, antihypoxic and neuroprotective effects.

show abstract

Effect of Peptide Semax on Synaptic Activity and Short-Term Plasticity of Glutamatergic Synapses of Co-Cultured Dorsal Root Ganglion and Dorsal Horn Neurons

Cited by 4 publications

References 11 publications

Effects of 1,8-cineole on neuropathic pain mediated by P2X2 receptor in the spinal cord dorsal horn

Effects of 1,8-cineole on neuropathic pain mediated by P2X2 receptor in the spinal cord dorsal horn

Lysine Dendrimers and Their Complexes with Therapeutic and Amyloid Peptides: Computer Simulation

Molecular Dynamics Simulation of Interaction of Short Lysine Brush and Oppositely Charged Semax Peptides

Contact Info

Product

Resources

About