Effect of oxiconazole and Ro 14-4767/002 on sterol pattern inCandida albicans

Polak-Wyss, A.; Lengsfeld, Hans; Oesterhelt, G.

doi:10.1080/00362178585380631

Cited by 25 publications

(9 citation statements)

References 24 publications

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“…For sediment slurries, low micromolar concentrations may be required (e.g., Fallon et al 1983, Fallon and Boylen 1990, van Duyl and Kop 1990). Note that other investigators have used 10-1-101 mmol/L added isotope-labeled precursors in order to obtain acceptably high activity in sterols or macromolecules in short (minutes to hours) incubations of fungi in culture or fungal enzyme systems (Pierce et al 1979, Arst and Scazzocchio 1980, Polak-Wyss et al 1985, Peacock and Goosey 1989, Henry 1990). One explanation for this is that fungal incorporation of 3H-thymidine into DNA-fraction-contaminating materials was rapidly rising with increased concentration of added thymidine, since fungal rate-saturation level might be expected to lie in the millimoles-per-litre range (Newell 1984; note that fungal mass is usually >98% of total microbial standing crop of standing-dead cordgrass: ).…”

Section: Methodsmentioning

confidence: 99%

“…It may be that millimolar concentrations of added labeled precursors are generally required to maximize assessment of fungal-synthesis rates in natural samples. Note that other investigators have used 10-'-10' mmol!L added isotope-labeled precursors in order to obtain acceptably high activity in sterols or macromolecules in short (minutes to hours) incubations of fungi in culture or fungal enzyme systems (Pierce et al 1979, Arst and Scazzocchio 1980, Polak-Wyss et al 1985, Peacock and Goosey 1989, Henry 1990).…”

Section: Methodsmentioning

confidence: 99%

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Toward A Method For Measuring Instantaneous Fungal Growth Rates In Field Samples

Newell¹,

Fallon²

1991

Ecology

174

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. Ecological Society of America is collaborating with JSTOR to digitize, preserve and extend access to Ecology.Abstract. Methods are available in the literature for the measurement of instantaneous growth rates in field samples of photosynthetic microbes and procaryotic saprotrophic microbes, but there has been no such method for eucaryotic saprotrophic microbes (members of the kingdom Fungi). We have devised a technique for estimating instantaneous growth rates for ergosterol-containing fungi in field material, and preliminarily applied the technique to estimation of fungal productivities and throughputs in two types of standingdead grass (one saltwater, one freshwater), obtaining plausible values (e.g., conversion efficiencies, total fungal production . leaf mass loss, were calculated to be 36-64%). This method is based on measuring rates of radiolabeled acetate incorporation into ergosterol. We examined several potential problem areas for use of radiolabeled precursors in measurement of microbial molecular syntheses, and its extrapolation to microbial productivity. Key findings were: (a) 5 mmol/L added acetate (required for maximization of detection of rates) had positive or neutral impact on 48-h fungal growth, and no shift-up (sudden upward change in rate) or lag in incorporation of acetate into ergosterol occurred between 0.25 and 1.25 h of incubation of naturally decaying leaf (Spartina alterniflora) samples with 5 mmol/L acetate; (b) bacterial assemblages on leaves did not contribute radioactivity to the ergosterol fraction after incubation with radioacetate (as opposed to > 30 Bq per centimetre length of leaf containing fungal mycelium); (c) the average empirical factor for conversion from nanomoles '4C-acetate incorporated to micrograms organic fungal mass produced was 8.2 ,ug/nmol (coefficient of variation, 27%). We tentatively conclude that the acetateto-ergosterol method is tenable for measurement of natural fungal productivity. et al. (1985) when they estimated procaryotic productivity by following the rate of incorporation of '4C-acetate into phospholipid fatty acids. We have tested whether measurement of rate of incorporation of radioacetate into ergosterol can be used in samples of decaying vascular plants (primarily smooth cordgrass, Spartina alterniflora Loisel.; see Newell et al. 1989), as a means of determining productivity of fungal decomposers in field samples. We have examined several areas of potential pitfalls (Staley and Konopka 1985, Karl 1986, Moriarty 1988 including ability of target microbes to take up the precursor molecule, concentrations of precursor required to maximize degree of participation (Bell 1990) of exogenous precursor, effect of presence...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Toward A Method For Measuring Instantaneous Fungal Growth Rates In Field Samples

Newell¹,

Fallon²

1991

Ecology

174

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“…AMF is known to inhibit ∆ 14 sterol reductase and ∆ 8 ¡∆ 7 sterol isomerase, and BFZ inhibits CYP51 A1 (sterol 14α-demethylase) in the ergosterol synthesis pathway. 5,6 Materials and methods …”

Section: Introductionmentioning

confidence: 99%

Actin Gene-Targeted RT-PCR Could be a Useful Method for Evaluating In Vitro Fungicidal Activity against Dermatophytes

Nimura¹,

Niwano²,

Ishiduka³

et al. 2003

J Int Med Res

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“…Amorolfine |4-{3-[/>-(l .l-dimethylpropyl)phenyl]-2-methyl-propyl}-2,6-c/.v-dimethylmorpholine hydrochlo ride] exhibits a broad spectrum of antifungal activity and is particularly effective in the topical therapy of superficial mycoses caused by yeasts as well as dermatophytes [1][2][3][4]. The mode of action has been described by Polak [5,6], Amorolfine inhibits the biosynthesis of ergosterol.…”

Section: Introductionmentioning

confidence: 99%

Influence of Amorolfine on the Morphology of Candida albicans and Trichophyton mentagrophytes

1992

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Amorolfine applied in concentrations of 0.1–100 μg/ml causes considerable damage to the ultrastructure of Candida albicans and Trichophyton mentagrophytes: electron-lucent areas appear in the cytoplasm. Extracytoplasmic membrane vesicles are formed and deposited in the cell wall. Starved fungal cells, with normal ultrastructure, can be found. Lysed, dead cells demonstrate the process of severe ultrastructural damage. T. mentagrophytes cell walls especially increase in thickness. The feature of the damage caused by amorolfine is comparable to that produced by azole antifungals.

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Effect of oxiconazole and Ro 14-4767/002 on sterol pattern inCandida albicans

Cited by 25 publications

References 24 publications

Toward A Method For Measuring Instantaneous Fungal Growth Rates In Field Samples

Toward A Method For Measuring Instantaneous Fungal Growth Rates In Field Samples

Actin Gene-Targeted RT-PCR Could be a Useful Method for Evaluating In Vitro Fungicidal Activity against Dermatophytes

Influence of Amorolfine on the Morphology of <i>Candida albicans</i> and <i>Trichophyton mentagrophytes</i>

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