Effect of oocyte-sperm co-incubation on acrosome reaction in the goat

Martín-Lunas, E.; Martino, A.; Paramio, M. T.; Palomo, M.J.; Mogas, M.T.; Bielsa, M.A.; Andolz, P.; Martı́nez, Paz

doi:10.1016/0093-691x(96)00188-4

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…Serum sources contain amino acids that play an important role as energy sources, osmoregulators, and pH stabilizers of maturation media (Bavister, 1995). Supplementation of maturation media with fetal bovine serum (Martin-Lunas et al, 1996), calf serum (Crozet et al, 1993), estrous goat serum (EGS) (Tajik and Shams Esfandabadi, 2003), bovine serum albumin (BSA) plus EGS (Rajikin et al, 1994) or BSA (Holm et al, 1999) as protein sources has been found to be beneficial for achieving high in vitro maturation (IVM), fertilization (IVF) and subsequent development (IVD) rates of immature oocytes.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Fertilization and Development of in Vitro Versus in Vivo Matured Rabbit Oocytes

Abdel-Khalek

Dora

Shamiah

et al. 2012

Egyptian Journal of Animal Production

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This study was carried out to evaluate the effect of adding two types of serum, bovine serum albumin (BSA) and doe rabbit serum (DRS) to maturation tissue culture medium on the in vitro maturation rate of follicular oocytes of rabbits, and to, evaluate the success rate of in vitro fertilization and development of in vivo versus in vitro mature rabbit oocytes. About 245 immature complete cumulus oocytes were recovered immediately from ovaries of sacrified rabbit does treated with PMSG (75 IU) for 48 h. Maturation medium supplemented with 10% DRS, 6% BSA or without serum (control) were used. In vitro (n= 245) and in vivo (n=264, collected from oviduct of does sacrified after 12 h of 0.02 ml GnRH injection) mature oocytes were used for in vitro fertilization and embryo development evaluation. Results revealed that TCM-199 plus DRS increased the percentage of oocytes at M I (25 vs.15%, P<0.05) and M II (55 vs. 47.5%, P>0.05) as compared to control TCM, respectively. However, TCM-199 plus BSA had more positive effect on in vitro maturation of oocyte in term of the lowest percentage of oocytes at germinal vesicles and germinal vesicles break down (0.0 and 9.4%) and the highest one (73%, P<0.05) at M II. Fertilization rate was higher (P<0.05) for in vivo than in vitro mature oocytes (86.3 vs. 62.5%). Percentage of embryos 4-cell stage decreased (18.3 vs. 34.7%, P<0.05) and at morula stage increased (30.5 vs. 16%, P<0.05) for in vivo as compared to in vitro matured oocytes. However, the percentages of embryos at 2-cell and 8~16-cell stages between in vivo and in vitro matured oocytes were not different. The current study obtained an appropriate rate of success for in vitro fertilization (62.5%) of in vitro mature rabbit oocytes with about 16% of them were developed to morula stage (transferable embryos) versus 86.3% of in vivo mature oocytes were fertilized with about 30.5% of them at morula stage. Based on the foregoing results of this study, addition of 10% of bovine serum albumin (BSA) to tissue culture medium had more beneficial effects on in vitro maturation rate of rabbit oocytes than that occurred with 10% of doe rabbit serum (DRS). In vitro maturation with 10% BSA showed success with satisfied in vitro fertilization rate and embryos at morula stage, but still to have lower developmental competence to fertilize in vitro than in vivo matured and in vitro fertilized oocytes.

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Section: Introductionmentioning

confidence: 99%

In Vitro Fertilization and Development of in Vitro Versus in Vivo Matured Rabbit Oocytes

Abdel-Khalek

Dora

Shamiah

et al. 2012

Egyptian Journal of Animal Production

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“…Sera are usually added to the IVM medium to the rate of 10-20% and are known to contain hormones, trace elements, growth factors [7], chelate heavy metal cations and serves as an osmolyte, as well as surfactant [8]. In all the experiments, maturation medium contained, bovine follicular fluid (BFF) [9], estrous goat serum (EGS) [10,11], estrous sheep serum (ESS) [11,12], estrous cow serum (ECS) [2], fetal bovine serum (FBS) [11,13] and bovine serum albumin (BSA) [14] as protein supplements. The maturation medium and Capsule Media Supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development.…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

Shabankareh

Sarsaifi

Mehrannia

2010

J Assist Reprod Genet

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Objective To compare the effect of human menopausal serum with estrous sheep serum, estrous goat serum, ovine follicular fluid and bovine follicular fluid on in vitro maturation, in vitro fertilization and embryo development of sheep oocytes Method (s) Oocytes were treated in culture with different sera and follicular fluids supplemented media to examine effects on embryo development. Results Basic culture medium supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development. Ovine follicular fluid and bovine follicular fluid supplementations supported similar rates of In vitro maturation, In vitro fertilization and embryo development which were lower than those supported by human menopausal serum, estrous sheep serum, estrous goat serum and control medium.Conclusion Human menopausal serum, estrous sheep serum, and estrous goat serum resulted in higher maturation, fertilization and embryo development than ovine follicular fluid, bovine follicular fluid and control media.

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In vitro maturation of caprine oocytes in different concentrations of estrous goat serum

Kharche

Goel

Jindal

et al. 2006

Small Ruminant Research

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Effect of oocyte-sperm co-incubation on acrosome reaction in the goat

Cited by 6 publications

References 23 publications

In Vitro Fertilization and Development of in Vitro Versus in Vivo Matured Rabbit Oocytes

In Vitro Fertilization and Development of in Vitro Versus in Vivo Matured Rabbit Oocytes

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

In vitro maturation of caprine oocytes in different concentrations of estrous goat serum

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