Effect of oocyte morphology on post-warming survival and embryo development in vitrified autologous oocytes

Coello, Aila; Sánchez, Esteban Morcillo; Vallejo, Begoña Iza; Meseguer, Marcos; Remohı́, José; Cobo, Ana

doi:10.1016/j.rbmo.2018.11.028

Cited by 11 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results from centres of expertise show survival rates of more than 95%, 92% and 85% in women who had oocytes vitrified at the age of <35 years, between 36-38 years, and >38 years, respectively. Oocytes of women of advanced age were more likely to be dysmorphic at vitrification and warming timepoints (Coello et al, 2019).…”

Section: Technical Advances In Cryobiologymentioning

confidence: 99%

Oocyte vitrification for elective fertility preservation: a SWOT analysis

Gil-Arribas¹,

Blockeel

Pennings

et al. 2022

Reproductive BioMedicine Online

View full text Add to dashboard Cite

Section: Technical Advances In Cryobiologymentioning

confidence: 99%

Oocyte vitrification for elective fertility preservation: a SWOT analysis

Gil-Arribas¹,

Blockeel

Pennings

et al. 2022

Reproductive BioMedicine Online

View full text Add to dashboard Cite

“…That vitrification and thawing, however, creates peripheral granulation as a fifth granulation pattern not seen in fresh oocytes, either in young donors or older infertility patients, strongly suggests a significant adverse effect of vitrification on oocytes, commensurate with lower pregnancy rates observed in national reporting data in comparison with that of fresh donor oocytes (36). Peripheral granulation clearly represents a sign of poor oocyte quality and of likely damage from vitrification and thawing procedures and thus could be used to assess improvements in vitrification techniques (42)(43)(44)(45). Because peripheral granulation uniformly de monstrated poor outcomes, similar to or even worse than those of dispersed granulation, which always resulted in poor outcomes in oocytes from fresh donors and poor-prognosis patients, embryos from donor oocytes with peripheral granulation, therefore, should likely be deselected whenever possible.…”

Section: Figurementioning

confidence: 96%

Predictive value of cytoplasmic granulation patterns during in vitro fertilization in metaphase II oocytes: part II, donor oocyte cycles

Molinari

Darmon

et al. 2021

Fertility and Sterility

View full text Add to dashboard Cite

Objective: To determine whether the ooplasm granulation patterns of donor oocytes, like those of oocytes from poor-prognosis patients, are predictive of in vitro fertilization (IVF) outcomes. Design: Retrospective cohort study. Setting: Academically affiliated private clinical infertility and research center. Patient(s): 770 fresh and 381 vitrified-thawed metaphase II oocytes from young donors (aged 21.0-34.6 years) used for IVF during 2017-2020. Intervention(s): Determination of granulation patterns in every oocyte during intracytoplasmic sperm injection as fine, central, uneven, dispersed, and peripheral (thawed only). Main Outcome Measure(s): Fertilization, pregnancy, and live birth rates in fresh and thawed donor oocytes. Both overall and knownoutcome analyses were performed for pregnancy and live birth. Result(s): In fresh donor oocytes, 2 pronuclei rates trended down from 96.1% to 90.2%, 88.9%, and 69.7% from fine to central, uneven, and dispersed granulations; overall pregnancy rates trended down from 50.4% to 29.0%, 17.7%, and 6.9%, as well as live birth rates (43.4%, 21.6%, 12.5%, and 6.4%), from fine to uneven, central, and dispersed granulations. Known pregnancy and known-live birth analyses showed similar findings. Thawed donor oocytes demonstrated similar trends in differences in fertilization, pregnancy, and live birth analyses with relatively worse outcomes. Peripheral granulation, unique to vitrification and thawing, always demonstrated the worst IVF outcomes. Moreover, granulation patterns were relatively disassociated from embryo morphological grades in fresh and largely disassociated in thawed donor oocytes. Conclusion(s):Predictive values of oocyte granulation patterns for fertilization, pregnancy, and live birth in IVF cycles are even more pronounced in young donors than results in older poor-prognosis patients, further supporting integration of oocyte granulation patterns into embryo selection.

show abstract

“…In the daily practice of ICSI, oocyte quality is evaluated by morphological parameters, including cytoplasmic granularity, zona pellucida, perivitelline space, etc. ; however, numerous studies have shown that human oocyte morphology is not significantly associated with competent, fertilization, subsequent embryo development or pregnancy outcomes. − Therefore, new techniques assessing oocyte quality are emerging, such as mitochondrial DNA copy number and transcript analysis of cumulus cells . It should be noted that these methods are invasive, which hinders their clinical application in the field of ART.…”

Section: Introductionmentioning

confidence: 99%

Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment

Dong,

Ouyang,

Lou

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

Nonobstructive azoospermia (NOA) is an important cause of infertility, and intracytoplasmic sperm injection (ICSI) is the mainstay of treatment for these patients. In cases where a sufficient number of sperm (usually 1−2) is not available, the selection of oocytes for ICSI is a difficult problem that must be solved. Here, we constructed a dual-activated oxidative stress-responsive AIE probe, b-PyTPA. The strong donor−acceptor configuration of b-PyTPA leads to twisted intramolecular charge transfer (TICT) effect that quenches the fluorescence of the probe, however, H 2 O 2 would specifically remove the boronatebenzyl unit and release a much weaker acceptor, which inhibits TICT and restores the fluorescence. In addition, the presence of a pyridine salt makes b-PyTPA more hydrophilic, whereas removal of the pyridine salt increases the hydrophobicity of PyTPA, which triggers aggregation and further enhances fluorescence. Thus, the higher the intracellular level of oxidative stress, the stronger the fluorescence. In vitro, this dual-activated fluorescent probe is capable of accurately detecting senescent cells (high oxidative stress). More importantly, b-PyTPA was able to characterize senescent oocytes, as assessed by the level of oxidative stress. It is also possible to identify high quality oocytes from those obtained for subsequent ICSI. In conclusion, this dual-activated oxidative stress-assessment probe enables the quality assessment of oocytes and has potential application in ICSI.

show abstract

Effect of oocyte morphology on post-warming survival and embryo development in vitrified autologous oocytes

Cited by 11 publications

References 33 publications

Oocyte vitrification for elective fertility preservation: a SWOT analysis

Oocyte vitrification for elective fertility preservation: a SWOT analysis

Predictive value of cytoplasmic granulation patterns during in vitro fertilization in metaphase II oocytes: part II, donor oocyte cycles

Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment

Contact Info

Product

Resources

About

Effect of oocyte morphology on post-warming survival and embryo development in vitrified autologous oocytes

Cited by 11 publications

References 33 publications

Oocyte vitrification for elective fertility preservation: a SWOT analysis

Oocyte vitrification for elective fertility preservation: a SWOT analysis

Predictive value of cytoplasmic granulation patterns during in vitro fertilization in metaphase II oocytes: part II, donor oocyte cycles

Dual-Activated H2O2-Responsive AIE Probes for Oocyte Quality Assessment

Contact Info

Product

Resources

About

Dual-Activated H₂O₂-Responsive AIE Probes for Oocyte Quality Assessment