Effect of novel curcumin‐encapsulated chitosan–bioglass drug on bone and skin repair after gamma radiation: experimental study on a Wistar rat model

Abstract

Radiation therapy contributes to a significant increase in bone osteoporosis and skin loss. Various natural health products might be beneficial to reduce bone and skin alterations. Curcumin (CUR) medicines derived from natural plants have played an important role in health care. This study aims at synthesizing and evaluating the performance therapy of CUR-encapsulated bioglass-chitosan (CUR-BG-CH). In vitro, the antioxidant assay was evaluated by using 1,1-diphenyl-2-picrylhydrazyl free-radical (DPPH) scavenging and the nitroblue tetrazolium reduction. The CUR-BG-CH antimicrobial effects were tested in liquid media. In vivo, after rat (60) Co γ-radiation, the tissue wound-healing process was studied by grafting CUR and CUR-BG-CH in femoral condyle and dorsal skin rat tissue. The antioxidant studies indicated that CUR-BG-CH quenches free radicals more efficiently than unmodified CUR and had effective DPPH (91%) and superoxide anion (51%) radical scavenging activities. The CUR-BG-CH biomaterial exhibited an important antimicrobial activity against Staphylococcus aureus. The histomorphometric parameters showed amelioration in CUR-BG-CH-treated rats. An improved mechanical property was noticed (33.16 ± 5.0 HV) when compared with that of unmodified CUR group (23.15 ± 4.9 HV). A significant decrease in tumour necrosis factor-α cytokine production was noted in the CUR-BG-CH rats (90 pg/ml) as compared with that of unmodified CUR group (240 pg/ml). The total amount of hydroxyproline was significantly enhanced (33.5%) in CUR-BG-CH group as compared with that of control. Our findings suggested that CUR-BG-CH might have promising potential applications for wound healing.

Antioxidant and Anti-Inflammatory Properties of Anthocyanins Extracted from Oryza sativa L. in Primary Dermal Fibroblasts

“…Numerous studies on the effects of natural or synthetic antioxidants on collagen deposition and on antioxidant defense have generated highly conflicting data, depending on the experimental system used. However, with various wound healing models, it has been repeatedly demonstrated that complex plant extracts containing active secondary metabolites (polyphenols, flavonoids, and alkaloids) [42, 43] and components of collagen-inducing polysaccharides, such as chitosan, and antioxidants such as curcumin [45] and resveratrol [46] ameliorated wound healing and increased skin collagen deposition, while suppressing proinflammatory markers. ANT extract from black soybean seed coats has been shown to decrease inflammation by suppressing the translocation of NF- κ B p65 into the nucleus of human dermal fibroblasts and keratinocytes [11].…”

Skin Antiageing and Systemic Redox Effects of Supplementation with Marine Collagen Peptides and Plant‐Derived Antioxidants: A Single‐Blind Case‐Control Clinical Study

“…Thus, with various wound healing models, it was repeatedly demonstrated that either complex plant extracts containing active secondary metabolites (triterpenes, polyphenols, alkaloids, etc.) [ 18 , 51 ] or a composition of collagen inducing polysaccharides like chitosan and antioxidants such as curcumin [ 52 ] or resveratrol [ 53 ] ameliorated wound healing increasing skin collagen deposition, while suppressing proinflammatory iNOS and myeloperoxidase, decreasing pathologically elevated levels of MDA and hydrogen peroxide, and improving enzymatic antioxidant defence. Recent studies showed that collagen peptides from fish skin remarkably promoted both wound healing and angiogenesis in different experimental settings [ 10 , 17 ].…”

Chitosan: An Update on Potential Biomedical and Pharmaceutical Applications

“…Cross-linked hydrogel films loaded with gentamicin sulfate displayed sustained drug release, in addition to effectively suppressing bacterial proliferation along with protecting the wound from infection [ 123 ]. Curcumin-encapsulated bioglass-chitosan, which is promising for wound healing applications, displayed higher 1,1-diphenyl-2-picrylhydrazyl and superoxide free radical quenching activities compared with unmodified curcumin, antibacterial activity against Staphylococcus aureus , and reduction in tumor necrosis factor- â production [ 124 ].…”