Effect of nordihydroguaiaretic acid on spermatogenesis and fertility in rats

Abbas, Manal A.; Badran, Darwish H.; Disi, Ahmad M.

doi:10.1111/and.12916

Cited by 7 publications

(2 citation statements)

References 46 publications

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“…After 700 μ M oxalate treatment for 24 h, cells were fixed in 5% glutaraldehyde at 4°C [ 21 ]. The cells were post-fixed with 1% osmium tetroxide for 1 h and embedded in epoxy resin.…”

Section: Methodsmentioning

confidence: 99%

Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK‐52E Cells Attenuates Oxalate‐Induced Intracellular Oxidative Stress

Jiang

Gao

Gong

et al. 2018

Oxidative Medicine and Cellular Longevity

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Solute carrier family 26 member 6 (Slc26a6), which is mainly expressed in the intestines and kidneys, is a multifunctional anion transporter that is crucial in the transport of oxalate anions. This study is aimed at investigating the effect of Slc26a6 expression on oxalate-induced cell oxidation and crystal formation. Lentivirus transfection was used to upregulate or downregulate Slc26a6 expression in NRK cells. Cell viability and apoptosis, reactive oxygen species (ROS) and malondialdehyde (MDA) generation, and superoxide dismutase (SOD) activity were measured. Crystal adhesion and the cell ultrastructure were observed using light and transmission electron microscopy (TEM). Three groups of rats, normal control, lentivirus-vector, and lentivirus-small interfering RNA (lv-siRNA) groups, were used, and after lentivirus transfection, they were fed 1% ethylene glycol (EG) and 0.5% ammonium chloride (NH4Cl) for 2 weeks. Dihydroethidium (DHE), terminal deoxynucleotidyl transferase (TdT) deoxyuridine dUTP nick-end labeling (TUNEL), and von Kossa staining were performed, and nuclear factor κB (NFκB) and osteopontin (OPN) expression were measured. In the vitro study, compared to the control group, downregulated Slc26a6 NRK cells showed alleviation of the cell viability decrease, cell apoptosis rate, ROS generation, and SOD activity decrease after oxalate treatment. Crystal adhesion and vesicles were significantly less after oxalate exposure than in the untreated controls. Rats infected with lentivirus-siRNA exhibited attenuated SOD generation, cell apoptosis, and crystal formation in the kidneys. Increased phosphorylation of NFκB and OPN was involved in the pathological process. In conclusion, the results of the present study indicate that reducing the expression of Slc26a6 in the kidney may be a potential strategy for preventing stone formation.

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“…After 700 μ M oxalate treatment for 24 h, cells were fixed in 5% glutaraldehyde at 4°C [ 21 ]. The cells were post-fixed with 1% osmium tetroxide for 1 h and embedded in epoxy resin.…”

Section: Methodsmentioning

confidence: 99%

Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK‐52E Cells Attenuates Oxalate‐Induced Intracellular Oxidative Stress

Jiang

Gao

Gong

et al. 2018

Oxidative Medicine and Cellular Longevity

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show abstract

“…After 4 weeks of BB or vehicle administration, fertility test was implemented according to the previous study. 31 Briefly, each male rat was cohabitated with two untreated normal female rats for 1 week.…”

Section: Fertility Testmentioning

confidence: 99%

Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway

et al. 2020

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Background: Diabetes mellitus (DM) induces impairment of male reproductive system and is considered as a key factor that could partially provide an explanation for male infertility. Thus, understanding the mechanism underlying DM-induced infertility will aid in the identification of novel therapeutic stratagems. Objectives: To delineate the role of ROS/JAK2/NFκB pathway in DM-induced low reproductive function and impaired spermatogenesis. Additionally, to investigate the protective effect of monomeric Berberine (BB) that inhibits ROS/JAK2/NFκB pathway, in the pathogenesis of DM-induced infertility.Methods: 12-week-old male Sprague-Dawley rats were divided into four groups: control group, DM group, control plus BB group, and DM plus BB group. Streptozotocin was used to induce DM. After treating the rats with BB for 4 weeks, fertility tests were conducted to investigate the reproductive function, and testis weight along with sperm motility was assessed through microscope. Oxidative stress was evaluated by DHE staining. TUNEL staining was utilized to detect the state of apoptosis. Cell experiments were carried out to define the role of BB in vitro. Immunohistochemistry, immunofluorescence, and Western blotting were employed to measure the protein expression. Results:Our results indicate that the reproductive function of DM rats was low, accompanied by decreased testis weight and sperm motility in addition to the impairment of the seminiferous tubules. However, there was a significant improvement in the reproductive function parameters in the BB-treated DM rats. Subsequently, our data revealed that DM rats produce an increased level of ROS in the testis, which activates JAK2 further activating the NFκB pathway, leading to increased apoptosis and impaired cells in the testicles. However, BB could attenuate the ROS production and abrogate activation of JAK2/NFκB pathway, thus inhibiting the apoptosis in the testicular cells of DM rats.Conclusion: ROS/JAK2/NFκB pathway is involved in the DM-induced low reproductive function and impaired spermatogenesis. BB can play a protective role in

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Exploring nordihydroguaretic acid (NDGA) as a plausible neurotherapeutic in the experimental paradigm of autism spectrum disorders targeting nitric oxide pathway

2021

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Effect of nordihydroguaiaretic acid on spermatogenesis and fertility in rats

Cited by 7 publications

References 46 publications

Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK‐52E Cells Attenuates Oxalate‐Induced Intracellular Oxidative Stress

Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK‐52E Cells Attenuates Oxalate‐Induced Intracellular Oxidative Stress

Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway

Exploring nordihydroguaretic acid (NDGA) as a plausible neurotherapeutic in the experimental paradigm of autism spectrum disorders targeting nitric oxide pathway

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