Effect of Nandrolone decanoate induced-oxidative stress on rat testes, prostate, and seminal vesicle: Biochemical, morphometric and histopathological studies

Saddick, Salina

doi:10.1016/j.sjbs.2020.09.039

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…In the present study, ND decanoate injection at a dose of 2.5mg/kg led to insignificant decrease of body weight and testicular weight. This result agreed with Saddick [12] , who showed that chronic administration of ND significantly decreased the body weight in male rats, this significance may be due to the more prolonged duration. The present study revealed that (ND) injection at a dose of 2.5mg/kg led to an insignificant decrease in the serum level of testosterone, FSH and LH.…”

Section: Discussionsupporting

confidence: 91%

Effect of Anabolic Steroid “Nandrolone” on Testes of Adult Albino Rats: Immunohistochemical and Ultrastructural Study

IBRAHIM¹,

SHAHAT²,

Bondok

2022

The Medical Journal of Cairo University

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Background: The Anabolic-androgenic steroids (AAS) are synthetic compounds based on the testosterone hormone structure. They have been abused by athletes to increase the muscular mass. Aim of Study:This study was performed to investigate the biochemical and histological changes in testis of adult albino rat model using different doses of one of the injectable forms of the anabolic steroid nandrolone deconate (ND).Material and Methods: Forty adult albino rats were divided into four groups (ten rats per group), injected IM once a week for ten weeks as follow; group I (control) were injected with 0.1ml of peanut oil, group II, group III and group IV rats were treated respectively with ND in a dose 2.5, 5 and 10mg/kg body weight solved in 0.1ml peanut oil.Results: Results of this study showed that the body weight significantly decreased only in group IV compared to the control group, a significant decrease in the testis weight in group III and IV while insignificantly changes in group II compared to group I. The blood level of testosterone, LH and FSH hormones insignificantly decreased in group II and III but significantly reduced in group IV compared to group I. Histological findings revealed decrease in the number of Leydig cells in the interstitial tissue, change in the shape of the seminiferous tubules, irregularity of the basement membrane with some areas detached from the germ cells, The germ cells showed vacuolated cytoplasm and decrease in number with subsequent dilatation of intercellular spaces and finally decreased sperms appearance in the lumen of the seminiferous tubules. These changes were noticed in all groups treated with ND to a variable degree being more evidenced in group III and IV and to less degree in group II compared to the group I. Conclusion:The results of this study showed that fine structures of the testis were affected by ND.

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Section: Discussionsupporting

confidence: 91%

Effect of Anabolic Steroid “Nandrolone” on Testes of Adult Albino Rats: Immunohistochemical and Ultrastructural Study

IBRAHIM¹,

SHAHAT²,

Bondok

2022

The Medical Journal of Cairo University

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“…Other studies also examined the impact of ND on the equilibrium of oxidants and antioxidants. Saddick (2021) reported malondialdehyde concentrations increased. Also, Mohamed and Mohamed (2015) evidenced the occurance of that oxidative damage through sperm parameters, maily increasing lipid peroxidation and decreasing antioxidant enzyme activities .…”

Section: Iv-discussionmentioning

confidence: 94%

Ameliorative Effect of Taurine on Nandrolone Decanoate Induced Toxicity on Brain, Heart and Testis in Adult Male Albino Rats

Megahed,

Abdel Moawed,

Abdel Aal

et al. 2024

Zagazig Journal of Forensic Medicine

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Background:The most prescribed anabolic steroid in gym practice is nandrolone decanoate (ND). It is implicated in sport doping, and criminal circles where personal aggressiveness was evident. Despite its wide use among athletes, its uncontrolled utilization causes serious toxicities of body organs. Taurine is an amino acid that is used as a supplement for bodybuilders and has many cytoprotective properties. Aim: This study aimed to evaluate the role of taurine in attenuation of the toxic effects of ND on biochemical parameters, histopathological and immune-histochemistry changes of hippocampus, heart and testis in adult male albino rats. Materials and Methods: It is an in vivo experimental study where 40 rats were classified into four groups; Control group, Taurine-treated, ND-treated group, and ND+Taurine group. Testicular levels of MDA, SOD, and TNFα, in addition to serum level of CK-MB were measured. Gene expression of testicular BAX, BCL2, MMP-9, Caspase-3, Cyp11a1, and hippocampal Prodynorphin were assessed. Hematoxylin and eosin (H&E), Masson trichrome stainings, immunohistochemical analysis and histo-morphometric studies were also done. Results: Taurine coadministration with ND significantly decreased levels of testicular MDA, TNF-α, serum CK-MB, and mRNA expression of testicular BAX, MMP-9, Caspase-3 and hippocampal Prodynorphin, with significant increase of testicular SOD level, and mRNA expression of testicular Cyp11a1 and a non-significant increase of testicular BCL2. The histopathological findings revealed marked amelioration of testicular and hippocampal toxicity, and significant decrease in collagen fibers in heart. Also, immunohistochemical expression of Ki-67 was increased in testis with decreased expression of GFAP in hippocampus comparable to ND-treated group. Conclusion: Taurine reduced the toxic effects of ND primarily on both the testis and hippocampus, in addition to a partial improvement in the heart.

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“…Saddick et al, (2021) studied the effect of ND-induced oxidative stress on rats, and the results of their study regarding testes weight and MDA were similar to the present study with decreased testes weights and increased MDA concentration among the ND-induced group. 16 The antioxidant Glutathione (GSH) showed a significant decrease among the ND-induced rats compared with the healthy group. Further, there was an increase in the GSH level in the treatment group as compared to the ND group as depicted in Figure 2(c).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

Sivanandam,

Senthilkumar,

Dipti

et al. 2024

Ind. J. Pharm. Edu. Res

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Background: Nandrolone decanoate (ND) is a commonly used androgen-based steroid that causes adverse effects on illicit self-administered users to boost their physical endurance. Although, it has an anabolic effect on the skeletal muscles, plausible evidence supports that the gonadal toxicity of ND can cause damage to reproductive functions. Aim: The present study investigated the possible protective effects of Withania somnifera (WS) root extract (200 and 400 mg/kg bw) on ND-induced (10 mg/kg bw) male gonadal toxicity in rats. Materials and Methods:The present seminal research was not experimented in any of the earlier studies. ND was induced in rats by intramuscular injection bi-weekly for 30 days. The aqueous extract of WS root powder was delivered orally once daily for 30 days to the ND-treated group. Hematoxylin-and-eosin-marked sections of the testes were studied. Results: The testosterone levels significantly decreased while there was an increase in the malondialdehyde levels among the ND-induced group against the control group. The ND induced rats treated with varying doses of WS showed a significant reversal of the gonadal toxicity by improvement in the testosterone and antioxidant parameters. The sperm count and sperm motility rate were increased significantly after WS therapy. Furthermore, the testis morphology of the WS-treated group indicated preserved germinal cells, active spermatogenesis, and absence of atrophy, vacuolation, and necrosis. Conclusion: WS treatment in ND-induced male rats alleviated the toxic effects of the ND on the gonads, probably due to its anti-inflammatory and antioxidant properties.

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Effect of Nandrolone decanoate induced-oxidative stress on rat testes, prostate, and seminal vesicle: Biochemical, morphometric and histopathological studies

Cited by 7 publications

References 34 publications

Effect of Anabolic Steroid “Nandrolone” on Testes of Adult Albino Rats: Immunohistochemical and Ultrastructural Study

Effect of Anabolic Steroid “Nandrolone” on Testes of Adult Albino Rats: Immunohistochemical and Ultrastructural Study

Ameliorative Effect of Taurine on Nandrolone Decanoate Induced Toxicity on Brain, Heart and Testis in Adult Male Albino Rats

Therapeutic Potential of Withania somnifera on Nandrolone Decanoate Induced Gonadal Toxicity

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