EFFECT OF MYCORRHIZAE AND ACTINOMYCETES ON GROWTH AND BIOPROTECTION OF Capsicum annuum L. AGAINST Phytophthora capsici

Abstract

To evaluate the inoculation effect of Arbuscular Mycorrhizal Fungi (AMF) and actinomycetes on promoting plant growth and diminishing wilt in pepper plant (Capsicum annuum L.), a pot experiment was conducted under greenhouse conditions using a factorial design on random blocks. It was evaluated the native Cerro del Metate mycorrhizal consortium, and ABV39 and ABV02 actinomycetes were evaluated against the CH11 stain of Phytophthora capsici. Responses of the variables evaluated were plant height, stem diameter, number of leaves, total fresh and dry biomass, leaf area and radical volume, number of AMF spores, mycorrhizal colonization, roots infected with P. capsici, and severity of wilting utilizing an ordinal qualitative scale. The results showed that mycorrhizal plants and inoculated with ABV39 demonstrated the highest values for plant height, stem diameter, and number of leaves with 26.12 cm, 5.29 mm, and 35.12 leaves, respectively. Co-inoculation of AMF and both actinomycetes significantly promoted (p≤0.001) total fresh and dry biomass and leaf area of the plants without P. capsici. The disease severity of the AMF-inoculated plants diminished against plants without mycorrhization. However, when they were also co-inoculated with ABV39 or ABV02, disease severity was significantly reduced (p≤0.05), up to level 2 of 5 levels on a severity scale. These results show a synergetic effect with co-inoculation of AMF and actinomycetes in vegetal growth promotion and bioprotection against wilting caused by P. capsici in pepper plants.

Hongos Micorrízicos Arbusculares en La Rizósfera De Plantas Nativas Y Su Efecto en El Crecimiento De Chile Poblano

“…The percentage of colonization by vesicles was relatively low and excels the mycorrhizal consortium of site 2 with 3.6 %, the lower colonization might be associated to the fact that the plants were evaluated when they were in an active stage of growth; thus, the development and the establishment of mycorrhizal symbiosis was in its early stages. Treatments showed significant statistical differences (Tukey, P ≤ 0.05) in the number of spores, being the consortia of the sites 4 (CS4) and 2 (CS2) the ones with the highest number of spores with 2590 and 2002 spores in 100 g of dry soil, the lowest value was found in site 5 (CS5) with 639 spores in 100 g of dry soil (Table 2); the spores quantified in this study are higher compared to other studies; for example Carballar-Hernández et al (2018) reported between 10 and 310 spores in Poblano pepper plants 90 days after inoculation, Reyes-Tena et al (2016) reported between 130 and 406 spores in the same culture but 56 days after transplantation, subsequently Reyes-Tena et al (2017) reported between 125 and 257 spores in Poblano pepper 86 days after transplantation.…”

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

“…Their relationship with biocontrol promoting actinomycetes has been gaining attention in the last years. Reyes-Tena et al (164) tested the synergistic effect of a mycorrhizal consortium with two strains of actinomycetes (ABV39 and ABV02) against Phytophthora capsici in pepper plants (Capsicum annuum L.). The results showed that the co-inoculation of the AMFs with the actinomycetes decreased the disease 3 points on a severity scale.…”

Degradation of soil arbuscular mycorrhizal fungal diversity and functionality accompanied by the aggravation of pepper Phytophthora blight in a facility shed in Southwest China

“…The protection of plants by AM fungi against a number of soil‐borne pathogens has been confirmed, and mechanisms such as nutritious/infection site competition, root anatomical/architectural alteration, and systemic resistance and mycorrhizosphere regulation have been ascribed (Hu et al, 2010; Wehner et al, 2010; Pozo et al, 2013; Li et al, 2021). The charming antagonistic effects of AM fungi on pepper Phytophthora blight through regulating plant immunity and/or rhizospheric environment have also been demonstrated (Alejo‐Iturvide et al, 2008; Hu et al, 2020; Ozgonen & Erkilic, 2007; Reyes‐Tena et al, 2017).…”