CCK8 cell proliferation experimentPulmonary adenocarcinoma cells H1299 were obtained, and the cells were transfected with Mir-299-3p, Mir-299-3p inhibitor and NC respectively. After 6 h of transfection, the medium was replaced with 1640 complete medium. The CCK8 method was used to measure cell proliferation, and the absorbance of each well at 450 nm was measured with a microplate reader.
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