Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells

Takita, T; Hayashi, Makoto; Takeichi, Osamu; Ogiso, Bunnai; Suzuki, Naoto; Otsuka, Kichibee; Ito, Kenji

doi:10.1111/j.1365-2591.2006.01097.x

Cited by 169 publications

(180 citation statements)

References 34 publications

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“…Although the effect of MTA on MC3T3-E1 cells was minimal, a significant difference between the groups was found on day 3. Our results were consistent with previously reports that MTA enables proliferation of various cell types [4][5][6][7][8] , and the excellent biocompatibility of MTA was confirmed.…”

Section: Discussionsupporting

confidence: 83%

“…The medium was collected on days 1, 2, and 3 after the placement of MTA. The concentration of calcium ions in the medium was assessed using the Calcium E-Test Kit (Wako Pure Chemical Industries, Osaka, Japan), as described previously 4) . Briefly, 1 mL Calcium E-Test reagent and 2 mL buffer were added to 50 µL of the collected medium, and the absorbance of the reaction products was measured at 610 nm using a microtiter plate reader (SpectraMax 190).…”

Section: Measurement Of Calcium Ions Released From Mtamentioning

confidence: 99%

“…Some in vitro studies have indicated that calcium ions are released continuously from MTA [10][11][12] . Takita et al reported that the release of ions from MTA provides the optimum amount of calcium for the proliferation and migration of dental pulp cells 4,13) . In another study involving undifferentiated mesenchymal cell cultures, calcium ions from MTA induced differentiation into osteoblast lineages 14) .…”

Section: Introductionmentioning

confidence: 99%

“…Thus, MTA has been widely used in endodontic treatments, including direct pulp capping, root-end filling, perforation repair, and apexification [1][2][3] . MTA bioactivity involves the ability to enhance cell proliferation, migration, and mineralization in various cell types [4][5][6][7][8][9] . MTA contains primarily calcium silicate, which is converted into calcium hydroxide upon contact with tissue fluid.…”

Section: Introductionmentioning

confidence: 99%

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Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Yasukawa

Hayashi

Tanabe

et al. 2017

Dental Materials Journal

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Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-E1 cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.

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Section: Discussionsupporting

confidence: 83%

Section: Measurement Of Calcium Ions Released From Mtamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Yasukawa

Hayashi

Tanabe

et al. 2017

Dental Materials Journal

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“…Furthermore, they have given little attention to the adverse effects of these materials on innate immune defense cells. 5,6 MTA has shown good performance as a repair material, by inducing bone, dentine, and cementum deposition. 7 Compared to Ca(OH) 2 cement, MTA has demonstrated a greater ability to maintain the integrity of pulp tissue by inducing the formation of a thicker dentin bridge, with a low inflammatory response.…”

Section: Introductionmentioning

confidence: 99%

Pulp capping materials exert an effect on the secretion of IL-1β and IL-8 by migrating human neutrophils

et al. 2011

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Pulp repair is a complex process whose mechanisms are not yet fully understood. The first immune cells to reach the damaged pulp are neutrophils that play an important role in releasing cytokines and in phagocytosis. The objective of this study was to analyze the effect of different pulp-capping materials on the secretion of interleukin-1 beta (IL-1β) and interleukin-8 (IL-8) by migrating human neutrophils. Neutrophils were obtained from the blood of three healthy donors. The experimental groups were calcium hydroxide [Ca(OH) 2 ], an adhesive system (Single Bond), and mineral trioxide aggregate (MTA). Untreated cells were used as control. Transwell chambers were used in performing the assays to mimic an in vivo situation of neutrophil chemotaxis. The pulpcapping materials were placed in the lower chamber and the human neutrophils, in the upper chamber. The cells were counted and the culture medium was assayed using ELISA kits for detecting and quantifying IL-1β and IL8. The data were compared by ANOVA followed by Tukey's test (p < 0.05). The secretion of IL-8 was significantly higher in all groups in comparison to the control group (p < 0.05). The adhesive system group showed higher IL-8 than the MTA group (p < 0.05). The secretion of IL-1β was significantly greater only in the MTA group (p < 0.001). It was concluded that only MTA is able to improve the secretion of IL-1β, and all materials tested increased IL-8 secretion. These results combined with all the other biological advantages of MTA indicate that it could be considered the material of choice for dental pulp capping.

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Materials for retrograde filling in root canal therapy

Jia

et al. 2016

Cochrane Database of Systematic Reviews

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Analysis 1.2. Comparison 1 MTA versus IRM, Outcome 2 Success rate-2-year outcome (participants as unit of analysis)............ Analysis 2.1. Comparison 2 MTA versus Super-EBA, Outcome 1 Success rate-1-year outcome (participants as unit of analysis)..... Analysis 3.1. Comparison 3 Super-EBA versus IRM, Outcome 1 Success rate-1-year outcome (teeth as unit of analysis)............. Analysis 4.1. Comparison 4 Dentine-bonded resin composite versus glass ionomer cement, Outcome 1 Success rate-1-year outcome PP analysis (participants as unit of analysis

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Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells

Abstract: In this ex-vivo study, the elution components such as calcium ions from MTA had higher proliferation ability of HDP cells than control and Dycal.

Cited by 169 publications

References 34 publications

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells

Pulp capping materials exert an effect on the secretion of IL-1β and IL-8 by migrating human neutrophils

Materials for retrograde filling in root canal therapy

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